全文获取类型
收费全文 | 586篇 |
免费 | 20篇 |
出版年
2022年 | 5篇 |
2021年 | 1篇 |
2020年 | 3篇 |
2019年 | 2篇 |
2018年 | 5篇 |
2017年 | 3篇 |
2016年 | 6篇 |
2015年 | 12篇 |
2014年 | 17篇 |
2013年 | 19篇 |
2012年 | 32篇 |
2011年 | 37篇 |
2010年 | 22篇 |
2009年 | 23篇 |
2008年 | 34篇 |
2007年 | 39篇 |
2006年 | 36篇 |
2005年 | 37篇 |
2004年 | 52篇 |
2003年 | 29篇 |
2002年 | 24篇 |
2001年 | 18篇 |
2000年 | 21篇 |
1999年 | 11篇 |
1998年 | 11篇 |
1997年 | 6篇 |
1996年 | 5篇 |
1995年 | 1篇 |
1994年 | 3篇 |
1993年 | 3篇 |
1992年 | 9篇 |
1991年 | 2篇 |
1990年 | 10篇 |
1989年 | 9篇 |
1988年 | 5篇 |
1987年 | 6篇 |
1986年 | 7篇 |
1985年 | 7篇 |
1984年 | 7篇 |
1983年 | 3篇 |
1982年 | 5篇 |
1981年 | 2篇 |
1980年 | 4篇 |
1979年 | 3篇 |
1978年 | 1篇 |
1976年 | 3篇 |
1975年 | 2篇 |
1972年 | 1篇 |
1970年 | 1篇 |
1968年 | 1篇 |
排序方式: 共有606条查询结果,搜索用时 31 毫秒
581.
Fujii A Ohsugi Y Yamamoto Y Nakamura T Sugiura T Tauchi M 《Journal of physiological anthropology》2007,26(3):381-385
In order to find out the most suitable and accurate pointing methods to study the sound localizability of persons with visual impairment, we compared the accuracy of three different pointing methods for indicating the direction of sound sources in a semi-anechoic dark room. Six subjects with visual impairment (two totally blind and four with low vision) participated in this experiment. The three pointing methods employed were (1) directing the face, (2) directing the body trunk on a revolving chair and (3) indicating a tactile cue placed horizontally in front of the subject. Seven sound emitters were arranged in a semicircle 2.0 m from the subject, 0 degrees to +/-80 degrees of the subject's midline, at a height of 1.2 m. The accuracy of the pointing methods was evaluated by measuring the deviation between the angle of the target sound source and that of the subject's response. The result was that all methods indicated that as the angle of the sound source increased from midline, the accuracy decreased. The deviations recorded toward the left and the right of midline were symmetrical. In the whole frontal area (-80 degrees to +80 degrees from midline), both the tactile cue and the body trunk methods were more accurate than the face-pointing method. There was no significant difference in the center (-40 degrees to +40 degrees from midline). In the periphery (-80 degrees and +80 degrees ), the tactile cue pointing method was the most accurate of all and the body trunk method was the next best. These results suggest that the most suitable pointing methods to study the sound localizability of the frontal azimuth for subjects who are visually impaired are the tactile cue and the body trunk methods because of their higher accuracy in the periphery. 相似文献
582.
A simple system was constructed and used in the experimental elucidation of the fate of a mutant emerging in a population. ThreeEscherichia coli strains having the same genetic background except for their glutamine synthetase gene were used as model competitors. The difference in the enzyme gene were introduced by random mutation. Competition between these bacterial strains was carried out and observed in a continuous liquid culture. In most cases, the competitors stably coexist either in a steady state or in an oscillating state. In addition, the competition between the strains was found to be a deterministic process and not a stochastic one. These results showed that an emerging mutant in a population, be it a closely related one to the original members, can attain a state of stable coexistence even in a homogeneous environment. The ability of each of the emerging mutants to maintain its stable coexistence with the original population gives rise to the accumulation of various mutants in a population. Therefore, evolution starts from gradual accumulation of various mutants in the population, which in turn leads to the diversification of the population. As our experimental system is a minimum model for the various competitions in the natural ecosystem, the observed competitive coexistence is proposed to be a general phenomenon in nature. 相似文献
583.
Ishii H. Roaki Hara Chinatsu Kashiwagi Keita Okabe Momoko Noguchi Yuiko Hirobe Muneto 《Landscape and Ecological Engineering》2022,18(1):1-10
Landscape and Ecological Engineering - Preventing invasion of exotic species into urban forests is important for sustaining native species diversity. In Japan, Ligustrum lucidum, an invasive tree,... 相似文献
584.
Y Nakao R Mukai T Kabashima Y Ohshima H Hamaguchi H Kashiwagi N Okada 《Biochemical and biophysical research communications》1982,109(4):1332-1338
We have isolated a nobel antibody from a patient with autoimmune disease which reacts with the ribonucleoprotein complex containing 7.5S RNA. The 7.5S RNA consists of two species having slightly different electrophoretic mobilities. Fingerprinting analysis of these two species demonstrates that nucleotide sequences of the RNAs are very similar to each other. Nucleotide composition of the 7.5S RNA is found to be; A/U/G/C=20/18/32/30, indicating that the ratio of GC content of the RNA(62%) is relatively high. The RNA contains a pseudouridylic acid residue as a modified nucleotide. Immunofluorescence pattern stained with the antibody suggests that the ribonucleoprotein complex containing 7.5S RNA is located both in the nucleolus and the cytoplasm. 相似文献
585.
W Kajiyama S Kashiwagi J Hayashi H Nomura H Ikematsu A Noguchi K Nakashima K Okochi 《Microbiology and immunology》1990,34(3):259-267
From 1983 to 1986, 1,813 children in nursery schools in Ishigaki Island and 1,228 children under 15 years old in the rural area in the Yaeyama District of Okinawa, Japan, were tested for anti-HTLV-I; 18 children (1.0%) in Ishigaki Island and 39 children (3.2%) in the rural area were positive. In order to survey when anti-HTLV-I developed in these children, their older serum samples were investigated retrospectively for 1 to 5 years. Two cases of seroconversion from anti-HTLV-I negative to positive were found between the age of 2 and 4, and there were no cases of seroconversion over 4 years old. Among the children who suffered maternal transmission of HTLV-I and developed anti-HTLV-I before the age of 15, about 80% of the children were considered to have anti-HTLV-I before the age of 2, and the remainder developed anti-HTLV-I before the age of 4. 相似文献
586.
S Kashiwagi H Ikematsu J Hayashi W Kajiyama H Nomura A Noguchi S Tani M Goto 《Microbiology and immunology》1988,32(9):917-923
A total of 2,283 serum samples were collected from healthy subjects in three islands of the Yaeyama district of Okinawa, Japan. These sera were tested for the presence of hepatitis B surface antigen (HBsAg), for antibody to hepatitis B core antigen (anti-HBc), and for antibody to adult T-cell leukemia-associated antigen (anti-ATLA). Correlation between hepatitis B virus infection and adult T-cell leukemia virus (ATLV) infection was determined by using the prevalence rates for three virus markers. Overall prevalence of HBsAg, anti-HBc and anti-ATLA was 6.5%, 57.4%, and 17.9%, respectively. Age-specific prevalence of anti-HBc and anti-ATLA increased with age, but that of HBsAg did not. Sex-specific prevalence of HBsAg was significantly higher in males than in females, but that of anti-ATLA was significantly higher in females than in males. Statistical analysis revealed that prevalence of anti-ATLA was significantly higher in HBsAg-positive persons and HBsAg-negative/anti-HBc-positive persons than in those negative for HBsAg and anti-HBc. These data suggest that hepatitis B virus-infected persons have a significantly higher chance of adult T-cell leukemia virus infection than those without hepatitis B virus infection in the area studied. 相似文献
587.
588.
589.
590.
Sawaki Tada Mayumi Matsushita-Morita Satoshi Suzuki Ken-Ichi Kusumoto & Yutaka Kashiwagi 《FEMS microbiology letters》2009,298(2):157-165
Ceramide is an important molecule not only structurally but also regulationally as a modulator of various cellular events. Ceramidase (CDase) are classified into three different types (acid, alkaline, and neutral CDases). Neutral CDase could play an important role in the regulation of ceramide levels in the extracellular space. In this study, we describe the characterization of a neutral CDase orthologue from the filamentous fungus Aspergillus oryzae . The gene encoding the neutral CDase orthologue was cloned and overexpressed in A . oryzae . The purified recombinant enzyme was optimally active at pH 4.0–4.5 and 40 °C. The apparent K m and V max values of the enzyme for C12-NBD-ceramide were 3.32 μM and 0.085 μmol min−1 mg−1 , respectively. 相似文献