全文获取类型
收费全文 | 322篇 |
免费 | 25篇 |
专业分类
347篇 |
出版年
2023年 | 1篇 |
2022年 | 1篇 |
2021年 | 2篇 |
2020年 | 1篇 |
2019年 | 1篇 |
2018年 | 3篇 |
2017年 | 1篇 |
2016年 | 4篇 |
2015年 | 11篇 |
2014年 | 8篇 |
2013年 | 14篇 |
2012年 | 22篇 |
2011年 | 20篇 |
2010年 | 13篇 |
2009年 | 8篇 |
2008年 | 15篇 |
2007年 | 23篇 |
2006年 | 14篇 |
2005年 | 21篇 |
2004年 | 22篇 |
2003年 | 13篇 |
2002年 | 12篇 |
2001年 | 15篇 |
2000年 | 22篇 |
1999年 | 15篇 |
1998年 | 2篇 |
1997年 | 3篇 |
1996年 | 3篇 |
1993年 | 1篇 |
1992年 | 8篇 |
1991年 | 4篇 |
1990年 | 8篇 |
1989年 | 11篇 |
1988年 | 10篇 |
1987年 | 4篇 |
1986年 | 1篇 |
1985年 | 4篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1982年 | 2篇 |
排序方式: 共有347条查询结果,搜索用时 15 毫秒
41.
42.
Imamura A Yoshikawa T Komori T Ando M Ando H Wakao M Suda Y Ishida H Kiso M 《Glycoconjugate journal》2008,25(3):269-278
A series of ganglioside GM1-, GM2-, and GM3-type probes, in which the ceramide portion is replaced with a glucose residue,
were systematically synthesized based on a convergent synthetic method. 相似文献
43.
Nishikawa M Yamamoto T Kojima N Kikuo K Fujii T Sakai Y 《Biotechnology and bioengineering》2008,99(6):1472-1481
The highly oxygen-permeable material, poly-dimethylsiloxane (PDMS), has the potential to be applied to cell culture microdevices, but cell detachment from PDMS has been a major problem. In this study, we demonstrate that a combination of collagen covalently immobilized PDMS and an adequate oxygen supply enables the establishment of a stable, attached spheroid (hemispheroid) culture of rat hepatocytes. The bottom PDMS surfaces were first treated with oxygen plasma, then coupled with aminosilane followed by a photoreactive crosslinker, and they were finally reacted with a collagen solution. X-ray photoelectron spectroscopy (XPS) and contact angle measurements showed that the covalent immobilization of collagen on the surface occurred only where the crosslinker had been introduced. On the collagen-conjugated PDMS surface, rat hepatocytes organized themselves into hemispheroids and maintained the viability and a remarkably high albumin production at least for 2 weeks of culture. In contrast, hepatocytes on the other types of PDMS surfaces formed suspended spheroids that had low albumin production. In addition, we showed that blocking the oxygen supply through the bottom PDMS surface inhibited the formation of hemispheroids and the augmentation of hepatocellular function. These results show that appropriate surface modification of PDMS is a promising approach towards the development of liver tissue microdevices. 相似文献
44.
Matsunaga I Komori T Ochi A Mori N Sugita M 《Biochemical and biophysical research communications》2008,377(1):165-169
Glycopeptidolipids (GPLs) comprise a major surface glycolipid of Mycobacterium avium complex (MAC), and their unique oligosaccharide extensions are known to define MAC serotypes. Beside the mature form of “serotype-specific” GPLs (ssGPLs), those that share the backbone structure but lack the oligosaccharide extensions exist as abundantly in all MAC serotypes, but the presumption was that antibody responses might not be directed to these “serotype-nonspecific” GPLs (nsGPLs) due to the lack of the sugar chain epitope. Here, we show that IgG responses to nsGPLs indeed occur in MAC-infected guinea pigs. The pool of anti-nsGPL antibodies was distinct from that of anti-ssGPL antibodies in terms of requirements for the oligosaccharide and acetylation for their target recognition. Because nsGPLs are shared in virtually all MAC strains, but totally absent in Mycobacterium tuberculosis, this study suggests that detecting serum anti-nsGPL antibodies can potentially be useful for differential diagnosis of MAC infection and tuberculosis. 相似文献
45.
I Masaki Y Yonemitsu K Komori H Ueno Y Nakashima K Nakagawa M Fukumura A Kato M K Hasan Y Nagai K Sugimachi M Hasegawa K Sueishi 《FASEB journal》2001,15(7):1294-1296
46.
T Matsuno T Maoka M Katsuyama T Hirono Y Ikuno M Shimizu T Komori 《Comparative biochemistry and physiology. B, Comparative biochemistry》1986,85(1):77-80
New luteins, lutein F [(3R,3'R,6'S)-beta,epsilon-carotene-3, 3'-diol] and lutein G [(3S,3'R,6'S)-beta,epsilon-carotene-3,3'-diol] have been isolated from marine fishes. 相似文献
47.
Ryuichi Higuchi Satomi Kubota Tetsuya Komori Toshio Kawasaki V.B. Pandey J.P. Singh A.H. Shah 《Phytochemistry》1984,23(11):2597-2600
Three new triterpenoid saponins were isolated from the barks of Zizyphus joazeiro and characterized as jujubogenin 3-O-α-l-arabinofuranosyl-(1 → 2)-[β-d-glucopyranosyl(1 → 3)]-α-l-arabinopyranoside, its 4?-O-sulphate and 3″,4?-di-O-sulphate, respectively. FAB-MS was useful in providing information on the molecular weight of the complex oligoglycoside sulphates. 相似文献
48.
Midori Kitayama Kiyohito Mizutani Masahiro Maruoka Kenji Mandai Shotaro Sakakibara Yuki Ueda Takahide Komori Yohei Shimono Yoshimi Takai 《The Journal of biological chemistry》2016,291(11):5817-5831
Mammary gland development is induced by the actions of various hormones to form a structure consisting of collecting ducts and milk-secreting alveoli, which comprise two types of epithelial cells known as luminal and basal cells. These cells adhere to each other by cell adhesion apparatuses whose roles in hormone-dependent mammary gland development remain largely unknown. Here we identified a novel cell adhesion apparatus at the boundary between the luminal and basal cells in addition to desmosomes. This apparatus was formed by the trans-interaction between the cell adhesion molecules nectin-4 and nectin-1, which were expressed in the luminal and basal cells, respectively. Nectin-4 of this apparatus further cis-interacted with the prolactin receptor in the luminal cells to enhance the prolactin-induced prolactin receptor signaling for alveolar development with lactogenic differentiation. Thus, a novel nectin-mediated cell adhesion apparatus regulates the prolactin receptor signaling for mammary gland development. 相似文献
49.
K Ohta M Kitada T Hashizume M Komori H Ohi T Kamataki 《Biochimica et biophysica acta》1989,996(1-2):142-145
Cytochrome P-450, designated as P-450-MK2, was purified to an electrophoretic homogeneity from polychlorinated biphenyl (PCB)-treated female crab-eating monkeys. P-450-MK2 catalyzed nifedipine and nilvadipine oxidations, at a rate comparable to human P-450-HM1. The N-terminal amino acid sequence of P-450-MK2 was highly homologous to those of P-450-HM1 and NF 25. The antibodies to P-450-HM1 recognized P-450-MK2 and effectively inhibited the activity of testosterone 6 beta-hydroxylase in monkey liver microsomes. These results suggest that a form of cytochrome P-450 corresponding to human P-450-HM1 or P-450NF which belongs to the P450 III gene family is also present in liver microsomes of crab-eating monkeys. 相似文献
50.
Isolation and Characterization of an Enterobacter cloacae Strain That Reduces Hexavalent Chromium under Anaerobic Conditions 总被引:6,自引:6,他引:6 下载免费PDF全文
Pi-Chao Wang Tsukasa Mori Kohya Komori Masanori Sasatsu Kiyoshi Toda Hisao Ohtake 《Applied microbiology》1989,55(7):1665-1669
An Enterobacter cloacae strain (HO1) capable of reducing hexavalent chromium (chromate) was isolated from activated sludge. This bacterium was resistant to chromate under both aerobic and anaerobic conditions. Only the anaerobic culture of the E. cloacae isolate showed chromate reduction. In the anaerobic culture, yellow turned white with chromate and the turbidity increased as the reduction proceeded, suggesting that insoluble chromium hydroxide was formed. E. cloacae is likely to utilize toxic chromate as an electron acceptor anaerobically because (i) the anaerobic growth of E. cloacae HO1 accompanied the decrease of toxic chromate in culture medium, (ii) the chromate-reducing activity was rapidly inhibited by oxygen, and (iii) the reduction occurred more rapidly in glycerol- or acetate-grown cells than in glucose-grown cells. The chromate reduction in E. cloacae HO1 was observed at pH 6.0 to 8.5 (optimum pH, 7.0) and at 10 to 40°C (optimum, 30°C). 相似文献