全文获取类型
收费全文 | 10329篇 |
免费 | 533篇 |
国内免费 | 4篇 |
出版年
2022年 | 53篇 |
2021年 | 111篇 |
2020年 | 55篇 |
2019年 | 83篇 |
2018年 | 115篇 |
2017年 | 107篇 |
2016年 | 152篇 |
2015年 | 241篇 |
2014年 | 284篇 |
2013年 | 612篇 |
2012年 | 512篇 |
2011年 | 456篇 |
2010年 | 299篇 |
2009年 | 298篇 |
2008年 | 482篇 |
2007年 | 445篇 |
2006年 | 459篇 |
2005年 | 413篇 |
2004年 | 421篇 |
2003年 | 447篇 |
2002年 | 394篇 |
2001年 | 414篇 |
2000年 | 407篇 |
1999年 | 339篇 |
1998年 | 143篇 |
1997年 | 108篇 |
1996年 | 85篇 |
1995年 | 84篇 |
1994年 | 77篇 |
1993年 | 88篇 |
1992年 | 243篇 |
1991年 | 248篇 |
1990年 | 197篇 |
1989年 | 180篇 |
1988年 | 185篇 |
1987年 | 158篇 |
1986年 | 148篇 |
1985年 | 146篇 |
1984年 | 121篇 |
1983年 | 108篇 |
1982年 | 92篇 |
1981年 | 58篇 |
1980年 | 59篇 |
1979年 | 91篇 |
1978年 | 84篇 |
1977年 | 61篇 |
1976年 | 57篇 |
1975年 | 49篇 |
1973年 | 58篇 |
1970年 | 49篇 |
排序方式: 共有10000条查询结果,搜索用时 46 毫秒
901.
Identification and characterization of TMEFF2, a novel survival factor for hippocampal and mesencephalic neurons 总被引:3,自引:0,他引:3
Horie M Mitsumoto Y Kyushiki H Kanemoto N Watanabe A Taniguchi Y Nishino N Okamoto T Kondo M Mori T Noguchi K Nakamura Y Takahashi Ei Tanigami A 《Genomics》2000,67(2):146-152
We have identified a novel mammalian gene, TMEFF2, that encodes a putative transmembrane protein containing two follistatin-like domains and one epidermal growth factor (EGF)-like domain. The TMEFF2 gene is predominantly expressed in the brain. In situ hybridization analysis revealed that TMEFF2 is widely expressed in the brain, including hippocampal cornu ammonis, dentate gyrus, and substantia nigra pars compacta. We evaluated the survival effect of TMEFF2 using primary cultured neurons from several regions of fetal rat brain following treatment with a recombinant TMEFF2 protein fragment consisting of the putative extracellular domain. TMEFF2 increased survival of neurons from the hippocampus and midbrain, but not from the cerebral cortex, indicating that the survival effects of TMEFF2 are specific to certain cell types. Recombinant TMEFF2 also promoted survival of mesencephalic dopaminergic neurons. Together, these findings suggest that TMEFF2 may be a novel survival factor for hippocampal and mesencephalic, but not for cortical, neurons. 相似文献
902.
The glucose isomerase from Streptomyces olivaceoviridis E-86 was purified by chromatographic procedures, showing one single protein band in the SDS-PAGE. The enzyme had high acid stability, and there was no loss in enzyme activity at pH 5.0 after incubation at 60 degrees C for 30 hr. The enzyme had sufficients activity at 60 degrees C, pH 5.5, (which is the reaction condition for a single-step process with a glucoamylase from A. niger), and at 58 degrees C, pH 6.0, (condition with a glucoamylase from R. niveus). By using this acid-stable glucose isomerase, a single-step process to produce high-fructose corn sweetener (HFCS) from liquefied starch was formed without any reductant or other reagents for enzyme stabilization. The HFCS produced was about fifty percent fructose and less than 1.5% unknown oligosaccharides. 相似文献
903.
Nagafuchi S Totsuka M Hachimura S Goto M Takahashi T Yajima T Kuwata T Kaminogawa S 《Bioscience, biotechnology, and biochemistry》2000,64(7):1459-1465
We have investigated the effects of dietary nucleotides on intraepithelial lymphocytes (IEL) and intestinal epithelial cells (IEC) in weanling mice. The proportion of T-cell receptor (TCR) gammadelta+ IEL in BALB/c mice fed a diet supplemented with nucleotides (NT(+) diet) was significantly higher than that in mice fed the nucleotide-free diet, while the proportion of TCR alphabeta+ IEL in NT(+) diet-fed mice was significantly decreased. The change of the TCR alphabeta+/TCR gammadelta+ ratio was mainly observed in a CD8 alphaalpha+ subset of IEL. IEC from NT(+) diet-fed mice produced a higher level of IL-7, which is important in the development of TCR gammadelta+ IEL, than those from control diet-fed mice. The expression levels of IL-7 and IL-2 receptors on IEL were not different between the two dietary groups. Our findings suggest that the increased population of a TCR gammadelta+ IEL subset by feeding nucleotides may be caused by the increased production of IL-7 by IEC. 相似文献
904.
Assessment of decalcifying protocols for detection of specific RNA by non-radioactive in situ hybridization in calcified tissues 总被引:6,自引:0,他引:6
Shibata Y Fujita S Takahashi H Yamaguchi A Koji T 《Histochemistry and cell biology》2000,113(3):153-159
For the best performance of in situ analysis of specific RNA expression in calcified tissues, it is necessary to choose an
appropriate protocol to decalcify the tissues. We evaluated the usefulness of various acid-based decalcifying reagents with
reference to 28 S rRNA staining by in situ hybridization using a thymine-thymine dimerized oligonucleotide probe. The reagents
evaluated were 10% nitric acid, 10% HCl, 5% formic acid, 5% trichloroacetic acid, Morse’s solution, Plank-Rychlo’s solution,
and K-CX solution, all of which are commonly used to decalcify tissues, and their effects on retention of morphology and RNA
were compared with EDTA-based solutions. When normal mouse mandible was used as a model tissue, well-preserved morphology
of ameloblasts was obtained from sections decalcified with Morse’s solution, 10% HCl, Plank-Rychlo’s solution, and K-CX solution,
and best retention of 28 S rRNA was obtained with 5% formic acid and Morse’s solution. We recommend Morse’s solution to decalcify
tissues to be processed for the rapid analysis of specific RNA expression. Indeed, we detected specific mRNAs strongly in
sections treated with Morse’s solution, and quantitative analysis showed that the ratio of signal intensities of 28 S rRNA
and the specific mRNAs correlated with each other depending on decalcifying solutions.
Accepted: 3 January 2000 相似文献
905.
Thermochemical transformation of sulfur compounds in Japanese domestic Allium, Allium victorialis L 总被引:1,自引:0,他引:1
Nishimura H Takahashi T Wijaya CH Satoh A Ariga T 《BioFactors (Oxford, England)》2000,13(1-4):257-263
Sulfur compounds contributed to the health promotion in Allium species are produced via enzymic and thermal reactions. Potent antithrombotic agents which have been identified as allyl trisulfides, dithiins, and ajoene in garlic (A. sativum) and caucas (A. victorialis) are thermochemically transformed from allicin (allyl 2-propenethiosulfinate). The leaves and stems of Japanese domestic Allium plant, A. victorialis L. which is widely distributed in the northern part of Japan, under the name "Gyoja-ninniku" is a nutritious vegetable. The significant flavor compounds of caucas are methyl allyl disulfide (Chinese chive odor), diallyl disulfide (garlic-like odor), and dimethyl disulfide and methyl allyl trisulfide (pickles-like odor) among more than 85 peaks on the gas chromatogram. 2-Vinyl-4H-1,3-dithiin and 3,4-dihydro-3-vinyl-1,2-dithiin as platelet aggregation inhibitors were found eliminated in dichloromethane extract of caucas. The significant health promoting factors, allyl trisulfides and dithiins were relatively increased when caucas was cooked on a frying pan. 相似文献
906.
907.
Yeast Smt3 is a ubiquitin-like protein similar to the mammalian SUMO-1. Cdc3, a septin component, is known to be modified by Smt3. The level of this modification was affected by Smt3-specific protease mutation ulp1-ts or overexpression of ULP1. By two-hybrid screening, we isolated 5 UIP (Ulp1 interacting protein) genes. UIP1 was identical to NUP42 encoding a component of the nuclear pore complex (NPC). Gle1, another NPC-associating component, also interacted with Ulp1 in the two-hybrid system and co-immunoprecipitation experiment. Thus Ulp1 associates with nucleoporins and may interact with septin rings in the telophase. 相似文献
908.
Metabolic pathways for cytotoxic end product formation from glutamate- and aspartate-containing peptides by Porphyromonas gingivalis
下载免费PDF全文
![点击此处可从《Journal of bacteriology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Metabolic pathways involved in the formation of cytotoxic end products by Porphyromonas gingivalis were studied. The washed cells of P. gingivalis ATCC 33277 utilized peptides but not single amino acids. Since glutamate and aspartate moieties in the peptides were consumed most intensively, a dipeptide of glutamate or aspartate was then tested as a metabolic substrate of P. gingivalis. P. gingivalis cells metabolized glutamylglutamate to butyrate, propionate, acetate, and ammonia, and they metabolized aspartylaspartate to butyrate, succinate, acetate, and ammonia. Based on the detection of metabolic enzymes in the cell extracts and stoichiometric calculations (carbon recovery and oxidation/reduction ratio) during dipeptide degradation, the following metabolic pathways were proposed. Incorporated glutamylglutamate and aspartylaspartate are hydrolyzed to glutamate and aspartate, respectively, by dipeptidase. Glutamate is deaminated and oxidized to succinyl-coenzyme A (CoA) by glutamate dehydrogenase and 2-oxoglutarate oxidoreductase. Aspartate is deaminated into fumarate by aspartate ammonia-lyase and then reduced to succinyl-CoA by fumarate reductase and acyl-CoA:acetate CoA-transferase or oxidized to acetyl-CoA by a sequential reaction of fumarase, malate dehydrogenase, oxaloacetate decarboxylase, and pyruvate oxidoreductase. The succinyl-CoA is reduced to butyryl-CoA by a series of enzymes, including succinate-semialdehyde dehydrogenase, 4-hydroxybutyrate dehydrogenase, and butyryl-CoA oxidoreductase. A part of succinyl-CoA could be converted to propionyl-CoA through the reactions initiated by methylmalonyl-CoA mutase. The butyryl- and propionyl-CoAs thus formed could then be converted into acetyl-CoA by acyl-CoA:acetate CoA-transferase with the formation of corresponding cytotoxic end products, butyrate and propionate. The formed acetyl-CoA could then be metabolized further to acetate. 相似文献
909.
910.
Fujino T Kojima M Beppu M Kikugawa K Yasuda H Takahashi K 《Journal of biochemistry》2000,127(6):1087-1093
Amino acid sequences in H(2)O(2)-oxidized bovine serum albumin (BSA) that are susceptible to proteolytic cleavage by oxidized protein hydrolase (OPH) were investigated. When oxidized BSA was treated with OPH, low-molecular-weight fragments (54, 46, 24, 22, 20, and 8 kDa) were produced as analyzed by SDS-PAGE. N-Terminal amino acid sequence analysis of these fragments indicated that oxidized BSA was cleaved by OPH at three major sites, Leu218-Ser219, Tyr410-Thr411, and Phe506-Thr507, at an early stage of the proteolytic degradation. In the three-dimensional structure of BSA deduced by computer modeling, these cleavage sites were found to be located slightly inside the BSA molecule, in positions not easily accessible by OPH. The influence of oxidation on the tertiary structure of BSA was then investigated by hypothetically replacing all the four methionine and two tryptophan residues with their oxidized forms, methionine sulfoxide and N'-formyl-kynurenine, respectively. The three-dimensional structure of the hypothetically oxidized BSA indicated that all the three cleavage sites in the protein could become more exposed to the solvent than in unoxidized BSA. These results suggest that, upon oxidation of BSA, the amino acid sequences that are potentially cleavable by OPH but present inside the molecule become exposed on the surface and susceptible to proteolysis by OPH. This is the first report demonstrating the cleavage sites of oxidized protein by oxidized protein-selective protease, suggesting the possible mechanism of oxidized protein-selective degradation by the enzyme. 相似文献