全文获取类型
收费全文 | 1836篇 |
免费 | 185篇 |
出版年
2021年 | 21篇 |
2020年 | 20篇 |
2019年 | 15篇 |
2018年 | 18篇 |
2017年 | 15篇 |
2016年 | 33篇 |
2015年 | 46篇 |
2014年 | 43篇 |
2013年 | 95篇 |
2012年 | 83篇 |
2011年 | 82篇 |
2010年 | 52篇 |
2009年 | 40篇 |
2008年 | 61篇 |
2007年 | 58篇 |
2006年 | 61篇 |
2005年 | 65篇 |
2004年 | 52篇 |
2003年 | 53篇 |
2002年 | 64篇 |
2001年 | 42篇 |
2000年 | 52篇 |
1999年 | 42篇 |
1998年 | 31篇 |
1997年 | 31篇 |
1996年 | 29篇 |
1995年 | 22篇 |
1994年 | 29篇 |
1993年 | 25篇 |
1992年 | 51篇 |
1991年 | 58篇 |
1990年 | 44篇 |
1989年 | 42篇 |
1988年 | 39篇 |
1987年 | 42篇 |
1986年 | 42篇 |
1985年 | 39篇 |
1984年 | 43篇 |
1983年 | 27篇 |
1982年 | 20篇 |
1980年 | 15篇 |
1979年 | 18篇 |
1978年 | 16篇 |
1977年 | 20篇 |
1976年 | 14篇 |
1975年 | 19篇 |
1974年 | 16篇 |
1973年 | 15篇 |
1972年 | 12篇 |
1971年 | 17篇 |
排序方式: 共有2021条查询结果,搜索用时 31 毫秒
941.
Jesse Roth Frances M. Ashcroft Claes B. Wollheim Timothy J. Kieffer Alan D. Cherrington Richard N. Bergman Roy Taylor Sonia M. Najjar Oluf Pedersen Helga Ellingsgaard Jens Juul Holst Michael A. Nauck Takashi Kadowaki Michael P. Czech Silvia Corvera Alan R. Saltiel Barbara E. Corkey Mark A. Atkinson 《Cell metabolism》2021,33(4):692-699
942.
Michael E. Kort Robert N. Atkinson James B. Thomas Irene Drizin Matthew S. Johnson Matthew A. Secrest Robert J. Gregg Marc J.C. Scanio Lei Shi Ahmed H. Hakeem Mark A. Matulenko Mark L. Chapman Michael J. Krambis Dong Liu Char-Chang Shieh XuFeng Zhang Gricelda Simler Joseph P. Mikusa Chengmin Zhong Shailen Joshi Brian E. Marron 《Bioorganic & medicinal chemistry letters》2010,20(22):6812-6815
A series of aryl-substituted nicotinamide derivatives with selective inhibitory activity against the Nav1.8 sodium channel is reported. Replacement of the furan nucleus and homologation of the anilide linker in subtype-selective blocker A-803467 (1) provided potent, selective derivatives with improved aqueous solubility and oral bioavailability. Representative compounds from this series displayed efficacy in rat models of inflammatory and neuropathic pain. 相似文献
943.
944.
Laura N. Anderson Laurent Briollais Helen C. Atkinson Julie A. Marsh Jingxiong Xu Kristin L. Connor Stephen G. Matthews Craig E. Pennell Stephen J. Lye 《PloS one》2014,9(4)
Background
The hypothalamic-pituitary-adrenal (HPA) axis regulates stress responses and HPA dysfunction has been associated with several chronic diseases. Low birthweight may be associated with HPA dysfunction in later life, yet human studies are inconclusive. The primary study aim was to identify genetic variants associated with HPA axis function. A secondary aim was to evaluate if these variants modify the association between birthweight and HPA axis function in adolescents.Methods
Morning fasted blood samples were collected from children of the Western Australia Pregnancy Cohort (Raine) at age 17 (n = 1077). Basal HPA axis function was assessed by total cortisol, corticosteroid binding globulin (CBG), and adrenocorticotropic hormone (ACTH). The associations between 124 tag single nucleotide polymorphisms (SNPs) within 16 HPA pathway candidate genes and each hormone were evaluated using multivariate linear regression and penalized linear regression analysis using the HyperLasso method.Results
The penalized regression analysis revealed one candidate gene SNP, rs11621961 in the CBG encoding gene (SERPINA6), significantly associated with total cortisol and CBG. No other candidate gene SNPs were significant after applying the penalty or adjusting for multiple comparisons; however, several SNPs approached significance. For example, rs907621 (p = 0.002) and rs3846326 (p = 0.003) in the mineralocorticoid receptor gene (NR3C2) were associated with ACTH and SERPINA6 SNPs rs941601 (p = 0.004) and rs11622665 (p = 0.008), were associated with CBG. To further investigate our findings for SERPINA6, rare and common SNPs in the gene were imputed from the 1,000 genomes data and 8 SNPs across the gene were significantly associated with CBG levels after adjustment for multiple comparisons. Birthweight was not associated with any HPA outcome, and none of the gene-birthweight interactions were significant after adjustment for multiple comparisons.Conclusions
Our study suggests that genetic variation in the SERPINA6 gene may be associated with altered CBG levels during adolescence. Replication of these findings is required. 相似文献945.
946.
Transposase activity that mediates the mobility of class II transposable elements, is most commonly initiated by the assembly of higher order synaptic complexes, called transpososomes. The formation of these complexes, that contain the transposable element's DNA as well as two or more molecules of the transposase, is dependent on interactions between transposase molecules. Using the yeast Two-Hybrid system, we were able to identify three regions mediating multimerization of the Hermes transposase, an element used for germline transformation of insects belonging to the hAT family of transposable elements. One region facilitating protein binding of Hermes transposase molecules was found within the first 252 amino acids of the transposase. The second region was located at the C-terminus of the transposase, and was found to be specific for Hermes transposase multimerization. Amino acids 551-569 were not only required for multimerization but were also necessary for transposition of the element. The third region was located between amino acids 253 and 380 and was found to eliminate the non-specific protein binding ability of the N-terminal protein interaction region but was required for the specific protein binding ability of the C-terminal region of the transposase. Five point mutations affecting the structural integrity of the C-terminal multimerization region abolished or significantly reduced transpositional activity. The same region had been previously identified to mediate dimerization in Activator (Ac), another hAT element, indicating that hAT transposase multimerization is likely to be a prerequisite for mobility of their elements. 相似文献
947.
The cyst nematode Heterodera cajani is one of the major endemic diseases of pigeonpea, an important legume for food security and protein nutrition in India. It occurs in several pulse crops grown over a range of Indian agro climatic conditions but the extent of its intraspecific variation is inadequately defined. In view of this, 11 populations of Heterodera cajani were analyzed using morphometrics and the results correlated with those obtained from an AFLP approach using 24 primer pair combinations that amplified a total of 1278 AFLP markers. The cluster solution from this binary data indicated similarities for five populations that differed from those suggested by morphometrics. The differences obtained could not be related to geographic distance between populations. The data suggests that recent and long distance dispersal has occurred whose causes need to be defined to restrict further field introductions. Four AFLP primer pairs clustered the populations similarly to that generated using all 24 primer pairs. This simplified approach may provide a rapid basis for discriminating populations for their future management and help to check further distribution in agricultural trade. It may also have potential to determine differences in populations that relate to host range or virulence to resistance genes. 相似文献
948.
Philippa CR Strong Stewart J Hinchliffe Hannah Patrick Steve Atkinson Olivia L Champion Brendan W Wren 《BMC microbiology》2011,11(1):85
Background
In order to identify new virulence determinants in Y. pseudotuberculosis a comparison between its genome and that of Yersinia pestis was undertaken. This reveals dozens of pseudogenes in Y. pestis, which are still putatively functional in Y. pseudotuberculosis and may be important in the enteric lifestyle. One such gene, YPTB1572 in the Y. pseudotuberculosis IP32953 genome sequence, encodes a protein with similarity to invasin, a classic adhesion/invasion protein, and to intimin, the attaching and effacing protein from enteropathogenic (EPEC) and enterohaemorraghic (EHEC) Escherichia coli. 相似文献949.
Kok-Gan Chan Steve Atkinson Kalai Mathee Choon-Kook Sam Siri Ram Chhabra Miguel Cámara Chong-Lek Koh Paul Williams 《BMC microbiology》2011,11(1):51
Background
Cell-to-cell communication (quorum sensing (QS)) co-ordinates bacterial behaviour at a population level. Consequently the behaviour of a natural multi-species community is likely to depend at least in part on co-existing QS and quorum quenching (QQ) activities. Here we sought to discover novel N -acylhomoserine lactone (AHL)-dependent QS and QQ strains by investigating a bacterial community associated with the rhizosphere of ginger (Zingiber officinale) growing in the Malaysian rainforest. 相似文献950.
Salmon JE Heuser C Triebwasser M Liszewski MK Kavanagh D Roumenina L Branch DW Goodship T Fremeaux-Bacchi V Atkinson JP 《PLoS medicine》2011,8(3):e1001013