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排序方式: 共有212条查询结果,搜索用时 8 毫秒
191.
Sai Kishore Ravi Yaoxin Zhang Yanan Wang Dilip Krishna Nandakumar Wanxin Sun Michael R. Jones Swee Ching Tan 《Liver Transplantation》2019,9(35)
Semiartificial photosynthetic systems have opened up new avenues for harvesting solar energy using natural photosynthetic materials in combination with synthetic components. This work reports a new, semiartificial system for solar energy conversion that synergistically combines photoreactions in a purple bacterial photosynthetic membrane with those in three types of transition metal–semiconductor Schottky junctions. A transparent film of a common transition metal interfaced with an n‐doped silicon semiconductor exhibits an in‐plane potential gradient when a light‐penetration variance is established on its surface by optical shading of photoabsorbing photosynthetic membranes. The in‐plane potential gradients (0.08–0.3 V) enable a directional charge transport between the synthetic and natural photoelectric systems, which is further enhanced in a device setting by a biocompatible thixotropic gel electrolyte that permeates the membrane multilayer, facilitating a strong and steady photoelectric current as high as 1.3 mA cm?2, the highest achieved so far with any anoxygenic photosynthetic system. 相似文献
192.
Programmed RNA breakage is an emerging theme underlying cellular responses to stress, virus infection and defense against foreign species. In many cases, site-specific cleavage of the target RNA generates 2′,3′ cyclic phosphate and 5′-OH ends. For the damage to be repaired, both broken ends must be healed before they can be sealed by a ligase. Healing entails hydrolysis of the 2′,3′ cyclic phosphate to form a 3′-OH and phosphorylation of the 5′-OH to form a 5′-PO4. Here, we demonstrate that a polynucleotide kinase-phosphatase enzyme from Clostridium thermocellum (CthPnkp) can catalyze both of the end-healing steps of tRNA splicing in vitro. The route of tRNA repair by CthPnkp can be reprogrammed by a mutation in the 3′ end-healing domain (H189D) that yields a 2′-PO4 product instead of a 2′-OH. Whereas tRNA ends healed by wild-type CthPnkp are readily sealed by T4 RNA ligase 1, the H189D enzyme generates ends that are spliced by yeast tRNA ligase. Our findings suggest that RNA repair enzymes can evolve their specificities to suit a particular pathway. 相似文献
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Efficacy of lipase from Aspergillus niger as an additive in detergent formulations: a statistical approach 总被引:1,自引:0,他引:1
Saisubramanian N Edwinoliver NG Nandakumar N Kamini NR Puvanakrishnan R 《Journal of industrial microbiology & biotechnology》2006,33(8):669-676
The efficacy of lipase from Aspergillus niger MTCC 2594 as an additive in laundry detergent formulations was assessed using response surface methodology (RSM). A five-level four-factorial central composite design was chosen to explain the washing protocol with four critical factors, viz. detergent concentration, lipase concentration, buffer pH and washing temperature. The model suggested that all the factors chosen had a significant impact on oil removal and the optimal conditions for the removal of olive oil from cotton fabric were 1.0% detergent, 75 U of lipase, buffer pH of 9.5 and washing temperature of 25°C. Under optimal conditions, the removal of olive oil from cotton fabric was 33 and 17.1% at 25 and 49°C, respectively, in the presence of lipase over treatment with detergent alone. Hence, lipase from A. niger could be effectively used as an additive in detergent formulation for the removal of triglyceride soil both in cold and warm wash conditions. 相似文献
195.
Nair PA Nandakumar J Smith P Odell M Lima CD Shuman S 《Nature structural & molecular biology》2007,14(8):770-778
Chlorella virus DNA ligase, the smallest eukaryotic ligase known, has pluripotent biological activity and an intrinsic nick-sensing function, despite having none of the accessory domains found in cellular ligases. A 2.3-A crystal structure of the Chlorella virus ligase-AMP intermediate bound to duplex DNA containing a 3'-OH-5'-PO4 nick reveals a new mode of DNA envelopment, in which a short surface loop emanating from the OB domain forms a beta-hairpin 'latch' that inserts into the DNA major groove flanking the nick. A network of interactions with the 3'-OH and 5'-PO4 termini in the active site illuminates the DNA adenylylation mechanism and the crucial roles of AMP in nick sensing and catalysis. Addition of a divalent cation triggered nick sealing in crystallo, establishing that the nick complex is a bona fide intermediate in the DNA repair pathway. 相似文献
196.
The filamentous nature and robust cell walls of many fungi render traditional measurements of active biomass (e.g., turbidity, dry cell weight) ineffective for most fungal bioprocesses. To overcome this challenge, an assay for quantification of overall metabolic activity is developed using 2,3-bis(2-methoxy-4-nitro-5-sulfophenly)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide (XTT), which in the presence of active mitochondria is converted to a water-soluble formazan derivative that absorbs light in the visible spectrum (430-490 nm). Tests on the model fungus Aspergillus nidulans show that in actively growing cultures XTT absorbance is linearly related to dry cell weight below 0.2 g/kg broth. Validation through growth rate testing shows the developed XTT assay is able to accurately quantify reductions in culture metabolism during damaging physical treatment (heat, high shear, microwaving). Experiments in batch culture demonstrate that the developed XTT assay is capable of reporting on metabolic activity where dry cell weight is not. The developed assay is inexpensive, relatively rapid, and easy to conduct, making it ideally suited for assessment of fungal processes in the biotechnology industry. 相似文献
197.
Hiroshi Hisano Rangaraj Nandakumar Zeng-Yu Wang 《In vitro cellular & developmental biology. Plant》2009,45(3):306-313
The energy in cellulosic biomass largely resides in plant cell walls. Cellulosic biomass is more difficult than starch to
break down into sugars because of the presence of lignin and the complex structure of cell walls. Transgenic down-regulation
of major lignin genes led to reduced lignin content, increased dry matter degradability, and improved accessibility of cellulases
for cellulose degradation. This review provides background information on lignin biosynthesis and focuses on genetic manipulation
of lignin genes in important monocot species as well as the dicot potential biofuel crop alfalfa. Reduction of lignin in biofuel
crops by genetic engineering is likely one of the most effective ways of reducing costs associated with pretreatment and hydrolysis
of cellulosic feedstocks, although some potential fitness issues should also be addressed. 相似文献
198.
Athira Athira Daniel Dondorp Jerneja Rudolf Olivia Peytral Marios Chatzigeorgiou 《PLoS biology》2022,20(8)
Vertebrate nervous systems can generate a remarkable diversity of behaviors. However, our understanding of how behaviors may have evolved in the chordate lineage is limited by the lack of neuroethological studies leveraging our closest invertebrate relatives. Here, we combine high-throughput video acquisition with pharmacological perturbations of bioamine signaling to systematically reveal the global structure of the motor behavioral repertoire in the Ciona intestinalis larvae. Most of Ciona’s postural variance can be captured by 6 basic shapes, which we term “eigencionas.” Motif analysis of postural time series revealed numerous stereotyped behavioral maneuvers including “startle-like” and “beat-and-glide.” Employing computational modeling of swimming dynamics and spatiotemporal embedding of postural features revealed that behavioral differences are generated at the levels of motor modules and the transitions between, which may in part be modulated by bioamines. Finally, we show that flexible motor module usage gives rise to diverse behaviors in response to different light stimuli.Vertebrate nervous systems can generate a remarkable diversity of behaviors, but how did these evolve in the chordate lineage? A study of the protochordate Ciona intestinalis reveals novel insights into how a simple chordate brain uses neuromodulators to control its behavioral repertoire. 相似文献
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