全文获取类型
收费全文 | 230篇 |
免费 | 15篇 |
国内免费 | 2篇 |
出版年
2024年 | 1篇 |
2023年 | 2篇 |
2022年 | 4篇 |
2021年 | 7篇 |
2020年 | 4篇 |
2019年 | 1篇 |
2018年 | 9篇 |
2017年 | 1篇 |
2016年 | 9篇 |
2015年 | 11篇 |
2014年 | 9篇 |
2013年 | 14篇 |
2012年 | 14篇 |
2011年 | 20篇 |
2010年 | 9篇 |
2009年 | 17篇 |
2008年 | 20篇 |
2007年 | 10篇 |
2006年 | 6篇 |
2005年 | 14篇 |
2004年 | 8篇 |
2003年 | 13篇 |
2002年 | 5篇 |
2001年 | 2篇 |
2000年 | 1篇 |
1999年 | 7篇 |
1998年 | 2篇 |
1997年 | 3篇 |
1996年 | 1篇 |
1995年 | 2篇 |
1993年 | 1篇 |
1990年 | 2篇 |
1989年 | 2篇 |
1988年 | 2篇 |
1987年 | 4篇 |
1985年 | 2篇 |
1982年 | 1篇 |
1979年 | 1篇 |
1976年 | 1篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1972年 | 1篇 |
1967年 | 1篇 |
1958年 | 1篇 |
排序方式: 共有247条查询结果,搜索用时 78 毫秒
101.
Dorsaf Hmidi Chedly Abdelly Habib-ur-Rehman Athar Muhammad Ashraf Dorsaf Messedi 《Physiology and Molecular Biology of Plants》2018,24(6):1017-1033
The short time response to salt stress was studied in Cakile maritima. Plants were exposed to different salt concentrations (0, 100, 200 and 400 mM NaCl) and harvested after 4, 24, 72 and 168 h of treatment. Before harvesting plants, tissue hydration, osmotic potential, inorganic and organic solute contents, and ornithine-δ-aminotransferase activity were measured. Plants of C. maritima maintained turgor and tissue hydration at low osmotic potential mainly at 400 mM NaCl. The results showed that, in leaves and stems, Na+ content increased significantly after the first 4 h of treatment. However, in roots, the increase of Na+ content remained relatively unchanged with increasing salt. The K+ content decreased sharply at 200 and 400 mM NaCl with treatment duration. This decrease was more pronounced in roots. The content of proline and amino acids increased with increasing salinity and treatment duration. These results indicated that the accumulation of inorganic and organic compounds was a central adaptive mechanism by which C. maritima maintained intracellular ionic balance under saline conditions. However, their percentage contribution to total osmotic adjustment varies from organ to organ; for example, Na+ accumulation mainly contributes in osmotic adjustment of stem tissue (60%). Proline contribution to osmotic adjustment reached 36% in roots. In all organs, proline as well as δ-OAT activity increased with salt concentration and treatment duration. Under normal growth conditions, δ-OAT is mainly involved in the mobilization of nitrogen required for plant growth. However, the highly significant positive correlation between proline and δ-OAT activity under salt-stress conditions suggests that ornithine pathway contributed to proline synthesis. 相似文献
102.
103.
104.
105.
Edward J Ciaccio Christina A Tennyson Govind Bhagat Suzanne K Lewis Peter HR Green 《Biomedical engineering online》2010,9(1):44
Background
Quantitative disease markers were developed to assess videocapsule images acquired from celiac disease patients with villous atrophy, and from control patients. 相似文献106.
107.
108.
“Chromosome conformation capture” (3C) is a powerful method to detect physical interaction between any two genomic loci. 3C involves formaldehyde crosslinking to stabilize transient interactions, followed by restriction digestion, ligation and locus-specific PCR. Accordingly, 3C reveals complex three-dimensional interactions between distal genetic elements within intact cells at high resolution. Here, we describe a modified 3C protocol designed for detection of transient chromatin interactions in the yeast Saccharomyces cerevisiae. Using this protocol, we are able to detect juxtaposition of promoter and terminator regions of genes with ORFs as short as 1 kb in length. We anticipate that this method will be generally applicable to detect dynamic, short-range chromatin interactions and will facilitate the characterization of gene loops and their functional consequences. 相似文献
109.
Human β-mannosidase (MANB) was purified to homogeneity directly from lysosomes by using mannosamine conjugated magnetic (Fe3O4) nanoparticles, DE-52 cellulose, and sephadex G-200 chromatography. Fe3O4 nanoparticles were synthesized and utilized ammonia to attach the amino group on the nanoparticles. The particles were covalently
attached with D-mannosamine by cross linker glutaraldehyde and confirmed by FTIR spectroscopy. In FTIR analysis, the peaks
appeared at 2,356.6 cm−1 for −N = CH linkage and at 3,378.4 cm−1, 3,664.9 cm−1 for −OH groups confirmed the conjugation of D-mannosamine with Fe3O4 nanoparticles. Results showed a single band of 97 kDa of purified MANB in SDS-PAGE. The isoelectric point was 4.5 and the
Km and Vmax values were 2.51 mM and 0.315 μM/min/mg, respectively. The purification fold was 329 with 68% yield. The optimal activity
was at pH 5.0 and 75% activity was stable in 20% glycerol at 4°C. The enzyme activity was inhibited by Ni2+, Zn2+, Cd2+, Cu2+, Mo2+, Ag+1, iodoacetate, SDS, DMF, DMSO, ethanol, and acetone; slightly reduced by Pb2+, Co2+, EDTA, DTT, and β-mercaptoethanol. The activity was not affected by Mg2+, Mn2+, Sn2+, Ca2+, Fe3+, PMSF, Triton X-100, D-mannosamine, D-mannose, D-mannitol, D-glucose, and D-fructose. The homogeneity of MANB enzyme was
further confirmed by 2D-PAGE and immunoblot. This is the first novel report of conjugation of D-mannosamine with Fe3O4 nanoparticles for purification of human MANB enzyme. 相似文献
110.
Sphingomyelin (SM) and ceramide-phosphoethanolamines (cer-PEs) are related lipids present in mammals and insects, respectively. Owing to the critical roles that cer-PEs play in eukaryotic cellular function, there is a need to develop methods that provide accurate quantitation of these compounds. Results obtained in this study demonstrate that Drosophila contains cer-PEs with unsaturated sphingoid base cores as well as low levels of cer-PEs that possess saturated sphingoid base cores. Specifically, the method developed in this study enabled the quantitation of picogram amounts of cer-PE containing both unsaturated d14:1Δ4 and d16:1Δ4 and saturated d14:0 sphingoid base cores. Using this method, cer-PE compounds with both saturated and unsaturated sphingoid base cores were initially identified by neutral loss scanning, followed by quantitation using selected reaction monitoring (SRM) scans. The SRM scans measured a product ion originating from the sphingoid base backbone, rather than from the head group, increasing the specificity and sensitivity of the quantitation measurement. 相似文献