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61.
Maria Braoudaki Fotini Tzortzatou-Stathopoulou Athanasios K. Anagnostopoulos Chrisa Papathanassiou Konstantinos Vougas Kalliopi Karamolegou George Th. Tsangaris 《Amino acids》2011,40(3):943-951
The aim of this study was to investigate the progression of myelodysplastic syndrome (MDS) to acute myeloid leukemia (AML)
and to provide additional data regarding the proteomic analysis of AML. The protein profiles obtained were correlated to cytogenetic
and molecular analyses. Bone marrow (BM) and peripheral blood (PB) samples were obtained during MDS diagnosis, at MDS transformation
to AML, at de novo AML diagnosis and 3 months following treatment. As controls, non-leukemic pediatric patients were studied.
Cytogenetic and molecular analyses were carried out by G banding and polymerase chain reaction followed by sequencing, respectively.
Differential proteomic analysis was performed by two-dimensional gel electrophoresis and protein identification by matrix-assisted
laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. No significant correlations were noted between protein
patterns and cytogenetic or molecular analyses. Certain suppressor genes, metabolic enzymes, immunoglobulins and actin-binding
proteins were differentially expressed by BM or PB plasma and cell lysates compared to controls. The obtained data showed
that vitamin D and gelsolin played contradicting roles in contributing and restraining leukemogenesis, while MOES, EZRI and
AIFM1 could be considered as biomarkers for AML. 相似文献
62.
63.
Anagnostopoulos AK Dimas KS Papathanassiou C Braoudaki M Anastasiadou E Vougas K Karamolegou K Kontos H Prodromou N Tzortzatou-Stathopoulou F Tsangaris GT 《Journal of proteome research》2011,10(5):2555-2565
Childhood pilocytic astrocytoma is the most frequent brain tumor affecting children. Proteomics analysis is currently considered a powerful tool for global evaluation of protein expression and has been widely applied in the field of cancer research. In the present study, a series of proteomics, genomics, and bioinformatics approaches were employed to identify, classify and characterize the proteome content of low-grade brain tumors as it appears in early childhood. Through bioinformatics database construction, protein profiles generated from pathological tissue samples were compared against profiles of normal brain tissues. Additionally, experiments of comparative genomic hybridization arrays were employed to monitor for genetic aberrations and sustain the interpretation and evaluation of the proteomic data. The current study confirms the dominance of MAPK pathway for the childhood pilocytic astrocytoma occurrence and novel findings regarding the ERK-2 expression are reported. 相似文献
64.
Chartoumpekis DV Habeos IG Ziros PG Psyrogiannis AI Kyriazopoulou VE Papavassiliou AG 《Molecular medicine (Cambridge, Mass.)》2011,17(7-8):736-740
Fibroblast growth factor-21 (FGF21) is a pleiotropic protein involved in glucose, lipid metabolism and energy homeostasis, with main tissues of expression being the liver and adipose tissue. Brown adipose tissue (BAT) is responsible for cold-induced thermogenesis in rodents. The role of FGF21 in BAT biology has not been investigated. In the present study, wild-type C57BL/6J mice as well as a brown adipocyte cell line were used to explore the potential role of cold exposure and β3-adrenergic stimulation in the expression of FGF21 in BAT. Our results demonstrate that short-term exposure to cold, as well as β3-adrenergic stimulation, causes a significant induction of FGF21 mRNA levels in BAT, without a concomitant increase in FGF21 plasma levels. This finding opens new routes for the potential use of pharmaceuticals that could induce FGF21 and, hence, activate BAT thermogenesis. 相似文献
65.
Charalampos Dokos Zoi-Dorothea Pana Athanasios Tragiannidis 《Current fungal infection reports》2011,5(1):18-22
The members of Malassezia species, which may cause skin disorders in healthy individuals, are associated with both cutaneous and systemic disorders
in immunocompromised patients. These disorders include folliculitis, seborrheic dermatitis, catheter-related fungemia, and
deeply invasive infections. This review examines not only the risk factors and pathogenesis of this rare fungal infection
but also the clinical manifestations, diagnosis, and treatment of Malassezia infections in immunocompromised patients. Reports from several series of immunocompromised patients have shown low mortality
rates with appropriate treatment. 相似文献
66.
Controlled clinical trials are widely considered to be the vehicle to treatment discovery in cancer that leads to significant improvements in health outcomes including an increase in life expectancy. We have previously shown that the pattern of therapeutic discovery in randomized controlled trials (RCTs) can be described by a power law distribution. However, the mechanism generating this pattern is unknown. Here, we propose an explanation in terms of the social relations between researchers in RCTs. We use social network analysis to study the impact of interactions between RCTs on treatment success. Our dataset consists of 280 phase III RCTs conducted by the NCI from 1955 to 2006. The RCT networks are formed through trial interactions formed i) at random, ii) based on common characteristics, or iii) based on treatment success. We analyze treatment success in terms of survival hazard ratio as a function of the network structures. Our results show that the discovery process displays power law if there are preferential interactions between trials that may stem from researchers' tendency to interact selectively with established and successful peers. Furthermore, the RCT networks are "small worlds": trials are connected through a small number of ties, yet there is much clustering among subsets of trials. We also find that treatment success (improved survival) is proportional to the network centrality measures of closeness and betweenness. Negative correlation exists between survival and the extent to which trials operate within a limited scope of information. Finally, the trials testing curative treatments in solid tumors showed the highest centrality and the most influential group was the ECOG. We conclude that the chances of discovering life-saving treatments are directly related to the richness of social interactions between researchers inherent in a preferential interaction model. 相似文献
67.
Athanasios Tragiannidis Zoe Dorothea Pana Theodotis Papageorgiou Emmanuel Hatzipantelis Maria Hatzistilianou Fani Athanassiadou 《Journal of medical case reports》2011,5(1):407
Introduction
Transient myeloproliferative disorder is a hematologic abnormality characterized by an uncontrolled proliferation of myeloblasts in peripheral blood and bone marrow that primarily affects newborns and babies with Down syndrome. Tumor lysis syndrome is rarely associated with transient myeloproliferative disorder.Case presentation
Transient myeloproliferative disorder was diagnosed in a seven-day-old baby girl with Down syndrome, who was referred to our department due to hyperleukocytosis. Our patient developed tumor lysis syndrome, successfully treated with rasburicase, as a complication of transient myeloproliferative disorder resulting from rapid degradation of myeloid blasts after initiation of effective chemotherapy.Conclusions
Tumor lysis syndrome is rarely reported as a complication of transient myeloproliferative disorder. To the best of our knowledge, this is the first case of a newborn with Down syndrome and transient myeloproliferative disorder treated with rasburicase for developing tumor lysis syndrome.68.
69.
Athanasios V. Mousiolis Panagoula Kollia Chara Skentou Ioannis E. Messinis 《Central European Journal of Biology》2011,6(6):918-924
Background
Data of syncytin 1 and 2 env gene expression in human placenta and participation in the syncytialisation phenomena has been reported. However, there are not many studies on simultaneous changes in expression of both syncytins in culture. We sought evidence on the relative expression of syncytins and syncytin 1 receptors in trophoblast cell culture treated with a differentiation inducing factor (forskolin). 相似文献70.
Karadas F Pappas AC Surai PF Speake BK 《Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology》2005,141(2):244-251
Carotenoids in the diet of the laying hen are incorporated into the egg yolk and subsequently into the liver and other tissues of the chicken embryo. Since these pigments are known to provide a range of health benefits to a variety of animals, it is of interest to know whether the effects of maternally derived carotenoids are strictly limited to the embryonic period or if they persist in the progeny after hatching. The aim of this study is to compare the effectiveness of pre-hatch (from the hen's diet) with that of post-hatch (from the progeny's diet) supplementation with carotenoids on the carotenoid status of the chick during the first 4 weeks of post-hatch life. Hens were fed a control diet or a diet supplemented with a carotenoid-rich extract of alfalfa. Eggs from the supplemented hens contained up to 22 times more carotenoids than the controls. The concentration of carotenoids in the livers of chicks hatching from the enriched eggs was initially 29 times greater than in the control chicks. Hepatic carotenoid concentrations in chicks from enriched eggs maintained post-hatch on the control diet were sustained at higher values compared with chicks from control eggs that were fed post-hatch on the carotenoid-supplemented diet, for at least the first 7 days. However, by 14 days, the latter group had overtaken the former in terms of liver carotenoid levels. Thus, under these conditions, maternal effects predominate for at least the first week after hatching, whereas from 2 weeks onwards, the progeny's diet becomes the main determinant of its carotenoid status. Since the antioxidant and immunostimulatory roles of carotenoids are likely to be especially important during the immediate post-hatch period, maternal dietary intake of carotenoids may have important ramifications for the viability of the offspring. 相似文献