全文获取类型
收费全文 | 637篇 |
免费 | 38篇 |
国内免费 | 2篇 |
出版年
2023年 | 10篇 |
2022年 | 11篇 |
2021年 | 17篇 |
2020年 | 10篇 |
2019年 | 10篇 |
2018年 | 14篇 |
2017年 | 8篇 |
2016年 | 23篇 |
2015年 | 28篇 |
2014年 | 26篇 |
2013年 | 42篇 |
2012年 | 48篇 |
2011年 | 74篇 |
2010年 | 46篇 |
2009年 | 31篇 |
2008年 | 50篇 |
2007年 | 53篇 |
2006年 | 31篇 |
2005年 | 36篇 |
2004年 | 33篇 |
2003年 | 14篇 |
2002年 | 21篇 |
2001年 | 4篇 |
2000年 | 2篇 |
1999年 | 1篇 |
1998年 | 3篇 |
1997年 | 3篇 |
1996年 | 4篇 |
1995年 | 6篇 |
1994年 | 2篇 |
1993年 | 4篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1990年 | 2篇 |
1988年 | 2篇 |
1981年 | 2篇 |
1979年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1963年 | 1篇 |
排序方式: 共有677条查询结果,搜索用时 888 毫秒
651.
Georgios Renieris Dionysia-Eirini Droggiti Konstantina Katrini Panagiotis Koufargyris Theologia Gkavogianni Eleni Karakike Nikolaos Antonakos Georgia Damoraki Athanasios Karageorgos Labros Sabracos Antonia Katsouda Elisa Jentho Sebastian Weis Rui Wang Michael Bauer Csaba Szabo Kalliopi Platoni Vasilios Kouloulias Andreas Papapetropoulos Evangelos J. Giamarellos-Bourboulis 《PLoS pathogens》2021,17(3)
Hydrogen sulfide (H2S) has recently been recognized as a novel gaseous transmitter with several anti-inflammatory properties. The role of host- derived H2S in infections by Pseudomonas aeruginosa was investigated in clinical and mouse models. H2S concentrations and survival was assessed in septic patients with lung infection. Animal experiments using a model of severe systemic multidrug-resistant P. aeruginosa infection were performed using mice with a constitutive knock-out of cystathionine-γ lyase (Cse) gene (Cse-/-) and wild-type mice with a physiological expression (Cse+/+). Experiments were repeated in mice after a) treatment with cyclophosphamide; b) bone marrow transplantation (BMT) from a Cse+/+ donor; c) treatment with H2S synthesis inhibitor aminooxyacetic acid (ΑΟΑΑ) or propargylglycine (PAG) and d) H2S donor sodium thiosulfate (STS) or GYY3147. Bacterial loads and myeloperoxidase activity were measured in tissue samples. The expression of quorum sensing genes (QS) was determined in vivo and in vitro. Cytokine concentration was measured in serum and incubated splenocytes. Patients survivors at day 28 had significantly higher serum H2S compared to non-survivors. A cut- off point of 5.3 μΜ discriminated survivors with sensitivity 92.3%. Mortality after 28 days was 30.9% and 93.7% in patients with H2S higher and less than 5.3 μΜ (p = 7 x 10−6). In mice expression of Cse and application of STS afforded protection against infection with multidrug-resistant P. aeruginosa. Cyclophosphamide pretreatment eliminated the survival benefit of Cse+/+ mice, whereas BMT increased the survival of Cse-/- mice. Cse-/- mice had increased pathogen loads compared to Cse+/+ mice. Phagocytic activity of leukocytes from Cse-/- mice was reduced but was restored after H2S supplementation. An H2S dependent down- regulation of quorum sensing genes of P.aeruginosa could be demonstrated in vivo and in vitro. Endogenous H2S is a potential independent parameter correlating with the outcome of P. aeruginosa. H2S provides resistance to infection by MDR bacterial pathogens. 相似文献
652.
653.
Organic Solar Cells: An Alternative Strategy to Adjust the Recombination Mechanism of Organic Photovoltaics by Implementing Ternary Compounds (Adv. Energy Mater. 24/2015)
下载免费PDF全文
![点击此处可从《Liver Transplantation》网站下载免费的PDF全文](/ch/ext_images/free.gif)
654.
655.
656.
George Nikitas Christos Maniotis George Manolis Athanasios G. Manolis 《Indian pacing and electrophysiology journal》2013,13(3):131-133
We present an image of pseudo-polymorphic ventricular tachycardia recording on a 12-lead surface ECG Holter. Although at first glance the appearance of the recording resembled polymorphic ventricular tachycardia, careful investigation revealed normal electrocardiographic findings. 相似文献
657.
Molecular Diagnosis & Therapy - Ion channels have major regulatory functions in living cells. Apart from their role in ion transport, they are responsible for cellular electrogenesis and... 相似文献
658.
Joel Selkrig Megan Stanifer Andr Mateus Karin Mitosch Inigo BarrioHernandez Mandy Rettel Heeyoung Kim Carlos G P Voogdt Philipp Walch Carmon Kee Nils Kurzawa Frank Stein Clment Potel Anna Jarzab Bernhard Kuster Ralf Bartenschlager Steeve Boulant Pedro Beltrao Athanasios Typas Mikhail M Savitski 《Molecular systems biology》2021,17(2)
The severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is a global threat to human health and has compromised economic stability. In addition to the development of an effective vaccine, it is imperative to understand how SARS‐CoV‐2 hijacks host cellular machineries on a system‐wide scale so that potential host‐directed therapies can be developed. In situ proteome‐wide abundance and thermal stability measurements using thermal proteome profiling (TPP) can inform on global changes in protein activity. Here we adapted TPP to high biosafety conditions amenable to SARS‐CoV‐2 handling. We discovered pronounced temporal alterations in host protein thermostability during infection, which converged on cellular processes including cell cycle, microtubule and RNA splicing regulation. Pharmacological inhibition of host proteins displaying altered thermal stability or abundance during infection suppressed SARS‐CoV‐2 replication. Overall, this work serves as a framework for expanding TPP workflows to globally important human pathogens that require high biosafety containment and provides deeper resolution into the molecular changes induced by SARS‐CoV‐2 infection. 相似文献
659.
Costas Perdikaris Anna Ergolavou Evangelia Gouva Cosmas Nathanailides Athanasios Chantzaropoulos Ioannis Paschos 《Reviews in Fish Biology and Fisheries》2012,22(1):17-27
Ichthyofauna in the Hellenic freshwaters is quite diverse consisted of 161 species (including 28 non-natives), out of the
546 native species in Pan-European level. However, the status of many aquatic ecosystems is currently degraded as a result
of a variety of anthropogenic impacts. This has direct reflection on the conservation status of the indigenous and native
fish species whose populations are in many cases declining in favor of the invasive ones. Prussian carp (Carassius
gibelio) is probably the most dominant invasive species and currently is considered as naturalized in about 26 lake and river basins.
Its successful adaptation and proliferation is mainly attributed to its dual reproductive mode (allogynogenetic and gonochoristic),
the opportunistic-omnivorous feeding habits and the ability to withstand and flourish in adverse ecological conditions. Accordingly,
it exerts competitive, as well as destructive effects upon the indigenous fish species (sperm parasitism, antagonism for food,
spawning grounds and spawning substrates) and therefore, efforts are needed to sustain a relative balance of the populations.
This review aims to critically discuss the status of C.
gibelio in Greece, the intrinsic causes of establishment and the observed impacts on ecosystems. Finally, reasonable actions are
proposed related to exploitation of C.
gibelio stocks and a path model for the protection of native fish species and their ecosystems. 相似文献
660.
El-Bermawi Nagy Baxevanis Athanasios D. Abatzopoulos Theodore J. Van Stappen Gilbert Sorgeloos Patrick 《Hydrobiologia》2004,523(1-3):175-188
Hydrobiologia - Four Artemia populations from northern Egypt, a bisexual one from Wadi El-Natrun Lake, two coastal parthenogenetic ones from Borg El-Arab and El-Max saltworks and an inland... 相似文献