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681.
Joel Selkrig Megan Stanifer Andr Mateus Karin Mitosch Inigo BarrioHernandez Mandy Rettel Heeyoung Kim Carlos G P Voogdt Philipp Walch Carmon Kee Nils Kurzawa Frank Stein Clment Potel Anna Jarzab Bernhard Kuster Ralf Bartenschlager Steeve Boulant Pedro Beltrao Athanasios Typas Mikhail M Savitski 《Molecular systems biology》2021,17(2)
The severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is a global threat to human health and has compromised economic stability. In addition to the development of an effective vaccine, it is imperative to understand how SARS‐CoV‐2 hijacks host cellular machineries on a system‐wide scale so that potential host‐directed therapies can be developed. In situ proteome‐wide abundance and thermal stability measurements using thermal proteome profiling (TPP) can inform on global changes in protein activity. Here we adapted TPP to high biosafety conditions amenable to SARS‐CoV‐2 handling. We discovered pronounced temporal alterations in host protein thermostability during infection, which converged on cellular processes including cell cycle, microtubule and RNA splicing regulation. Pharmacological inhibition of host proteins displaying altered thermal stability or abundance during infection suppressed SARS‐CoV‐2 replication. Overall, this work serves as a framework for expanding TPP workflows to globally important human pathogens that require high biosafety containment and provides deeper resolution into the molecular changes induced by SARS‐CoV‐2 infection. 相似文献
682.
Costas Perdikaris Anna Ergolavou Evangelia Gouva Cosmas Nathanailides Athanasios Chantzaropoulos Ioannis Paschos 《Reviews in Fish Biology and Fisheries》2012,22(1):17-27
Ichthyofauna in the Hellenic freshwaters is quite diverse consisted of 161 species (including 28 non-natives), out of the
546 native species in Pan-European level. However, the status of many aquatic ecosystems is currently degraded as a result
of a variety of anthropogenic impacts. This has direct reflection on the conservation status of the indigenous and native
fish species whose populations are in many cases declining in favor of the invasive ones. Prussian carp (Carassius
gibelio) is probably the most dominant invasive species and currently is considered as naturalized in about 26 lake and river basins.
Its successful adaptation and proliferation is mainly attributed to its dual reproductive mode (allogynogenetic and gonochoristic),
the opportunistic-omnivorous feeding habits and the ability to withstand and flourish in adverse ecological conditions. Accordingly,
it exerts competitive, as well as destructive effects upon the indigenous fish species (sperm parasitism, antagonism for food,
spawning grounds and spawning substrates) and therefore, efforts are needed to sustain a relative balance of the populations.
This review aims to critically discuss the status of C.
gibelio in Greece, the intrinsic causes of establishment and the observed impacts on ecosystems. Finally, reasonable actions are
proposed related to exploitation of C.
gibelio stocks and a path model for the protection of native fish species and their ecosystems. 相似文献
683.
El-Bermawi Nagy Baxevanis Athanasios D. Abatzopoulos Theodore J. Van Stappen Gilbert Sorgeloos Patrick 《Hydrobiologia》2004,523(1-3):175-188
Hydrobiologia - Four Artemia populations from northern Egypt, a bisexual one from Wadi El-Natrun Lake, two coastal parthenogenetic ones from Borg El-Arab and El-Max saltworks and an inland... 相似文献
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685.
Yu-Shik Hwang Yunyi Kang Athanasios Mantalaris 《Biotechnology and Bioprocess Engineering》2007,12(1):15-21
Regeneration of skeletal tissues is among the most promising areas of biological repair, providing a broad spectrum of potential
clinical applications. In view of the ageing population and the worldwide shortage of donor tissue, tissue engineering is
expected to become a major contributor to modern medicine. Recently, embryonic stem cells (ESCs) have received extensive attention
due to their distinct biological properties, namely their unlimited self-renewal capacity and their pluripotency, which have
rendered them a potent cell source for various medical and tissue engineering applications. The application of embryonic stem
cells to skeletal tissue engineering requires inducing thein vitro differentiation of ESCs into the osteogenic and chondrogenic lineages. Although considerable progress has been made in directing
embryonic stem cell differentiation towards the osteogenic and chondrogenic lineages, there are still obstacles remaining
that need to be resolved before ESCs can be used as a suitable cell source in cell and tissue therapies. In particular, the
efficient differentiation of ESCsin vitro towards the desired lineage requires the development of well-defined and proficient protocols, which would reduce the likelihood
of spontaneous differentiation into divergent lineages and increase the available cell source for application to bone and
cartilage tissue engineering therapies. Herein, this review provides a critical examination of the various experimental strategies
that could be used to direct the differentiation of ESCs towards the skeletal tissuein vitro, especially the osteogenic and chondrogenic lineasges. 相似文献
686.
It is widely accepted that a healthy diet is an important factor in preventing chronic diseases, and in improving energy balance
and weight management. Studies have shown strong inverse correlations between tomato consumption and the risk of certain types
of cancer, cardiovascular diseases and age-related macular degeneration. Because tomato is the second-most important vegetable
in the world after potato, this horticultural crop constitutes an excellent source of health-promoting compounds due to the
balanced mixture of minerals and antioxidants including vitamins C and E, lycopene, β-carotene, lutein and flavonoids such
as quercetin. Improvement in phytonutrients in tomatoes can be achieved by cultivar selection, environmental factors, agronomic
practices, stage of ripeness at harvest, and appropriate handling and conditioning all the way from the field to the consumer.
The purpose of this paper is to review the recent literature of the main factors that can improve the nutritional quality
of tomato and consequently their beneficial role in human diet. The importance of genotype selection and the optimization
of environmental conditions (light, temperature, humidity, atmospheric CO2 and air pollutants) for high nutritional value is outlined first, followed by the optimization of agricultural practices
(soil properties, water quality, mineral nutrition, salinity, grafting, pruning, growing systems, growth promoters, maturity,
and mechanical and pest injuries). The review concludes by identifying several prospects for future research such as modelling
and genetic engineering of the nutritional value of tomato. 相似文献
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689.
Amyl nitrite was administered on two occasions to a patient with muscular subaortic stenosis, to ascertain if selective peripheral vasodilatation would affect the degree of obstruction to left ventricular outflow in this condition. On each occasion there was a marked increase in the systolic pressure gradient across the left ventricular outflow tract prior to the onset of reflex tachycardia. Following the second amyl nitrite inhalation, the systolic cross-sectional area of the left ventricular outflow tract decreased to 1.0 sq. cm., from the control value of 2.6 sq. cm. It is believed that the increased degree of muscular subaortic stenosis, following peripheral vasodilatation, could be secondary to a decrease in the systolic size of the whole left ventricle, or to a selective decrease in the physical size of the left ventricular outflow tract. 相似文献
690.
Maria Lazarina Stefanos P. Sgardelis Thomas Tscheulin Jelle Devalez Vangelis Mizerakis Athanasios S. Kallimanis Spyridon Papakonstantinou Thanassis Kyriazis Theodora Petanidou 《Biodiversity and Conservation》2017,26(1):115-131
Fire affects diversity and structure of flower-visitor communities. We explored the effect of fire history on the diversity patterns and structure of the flower-visitor guilds (bees, beetles, flies, sawflies, and wasps), across a post-fire regeneration sequence of pine forests in Rhodes Island, Greece. Fire history was defined by fire frequency and post-fire age of sites. We did not find significant differences in species richness and abundance per guild between fire-history regimes except for the abundance of flies, sawflies and the entire flower-visitor community. Fire strongly affected the community structure of bees, beetles, and sawflies. Some of the most abundant and common species responded significantly to fire in terms of abundance; yet, we observed no significant variation in the abundance of the common species of bees, the most prominent pollinator group, implying that provision of pollination services may not be considerably affected by fire. Long-unburnt sites displayed higher heterogeneity in species composition compared to recently-burnt sites (either burnt once or twice). This pattern deviated significantly from null expectations when analyzing the abundance-based metric of β-diversity and was obscure in the case of the presence/absence-based metric, indicating that fire affected mainly the spatial distribution of individuals, i.e. the main change is not due to species turnover but to changes in relative abundance. Furthermore, the species composition in recently-burnt sites (burnt once in 2008) were more similar than in twice-burnt sites (including areas burnt once between 1984 and 1992, and in 2008), indicating that fire frequency affected post-fire heterogeneity of species composition. 相似文献