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61.
Mashat M Chrystyn H Clark BJ Assi KH 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2008,869(1-2):59-66
A reversed-phase liquid chromatography method involving pre-column derivatisation with fluorescein isothiocyanate (FITC, isomer I) for determination of tobramycin in urine samples after inhalation has been developed. FITC reacts with the primary amino groups of tobramycin and other aminoglycosides under mild conditions to form a highly fluorescent and stable derivative. The chromatographic separation was carried out on a Phenomenex Luna C(18) column at ambient temperature using a constant flow rate of 1 ml/min and mobile phase of acetonitrile-methanol-glacial acetic acid-water (420:60:5:515, v/v/v/v). The tobramycin-FITC derivative was monitored by fluorescent detection at an excitation wavelength 490 nm and emission wavelength 518 nm. The linearity of response for tobramycin was demonstrated at 11 different concentrations of tobramycin extracted from spiked urine, ranging from 0.25 to 20 microg/ml. Tobramycin and neomycin were extracted from spiked urine by a solid phase extraction clean-up procedure on a carboxypropyl-bonded phase (CBA) weak cation-exchange cartridge, and the relative recovery was >99% (n=5). The limit of detection (LOD) and limit of quantitation (LOQ) in urine were 70 and 250 ng/ml, respectively. The method had an accuracy of <0.2%, and intra-day and inter-day precision (in term of %coefficient of variation) were <4.89% and 8.25%, respectively. This assay was used for urinary pharmacokinetic studies to identify the relative lung deposition of tobramycin post-inhalation of tobramycin inhaled solution 300 mg/5 ml (TOBI) by different nebuliser systems. 相似文献
62.
Denise Cazzato Emma Assi Claudia Moscheni Silvia Brunelli Clara De Palma Davide Cervia Cristiana Perrotta Emilio Clementi 《Experimental cell research》2014
The muscle-specific variant of neuronal nitric oxide (NO) synthase (NOS-I), is developmentally regulated in mouse suggesting a role of NO during myogenesis. In chick embryo, a good model of development, we found that the expression of NOS-I is up-regulated, but only in the early phase of development. Through a pharmacological intervention in ovo we found that NO signalling plays a relevant role during embryonic development. The inhibition of NOS-I decreased the growth of embryo, in particular of muscle tissue, while the restoring of physiological NO levels, via administration of a NO donor, reversed this effect. We found a selective action of NO, produced by NOS-I, on regulatory factors involved in myogenic differentiation in the early phase of chick embryo development: inhibition of NO generation leads to a decreased expression of the Myocyte enhancer factor 2a (Mef2a), Mef2c, Myogenin and Myosin, which was reversed by the administration of a NO donor. NO had no effects on Myf5 and MyoD, the myogenic regulatory factors necessary for myogenic determination. The action of NO on the myogenic regulatory factors was mediated via generation of cyclic GMP (cGMP) and activation of the cGMP-dependent protein kinase G (PKG). Finally we found in myoblasts in vitro that the activation of Mef2c was the key event mediating the NO-induced modulation of myogenesis. 相似文献
63.
Perrotta C Bizzozero L Cazzato D Morlacchi S Assi E Simbari F Zhang Y Gulbins E Bassi MT Rosa P Clementi E 《The Journal of biological chemistry》2010,285(51):40240-40251
Acid sphingomyelinase (A-SMase) is an important enzyme in sphingolipid metabolism and plays key roles in apoptosis, immunity, development, and cancer. In addition, it mediates cytotoxicity of cisplatin and some other chemotherapeutic drugs. The mechanism of A-SMase activation is still undefined. We now demonstrate that, upon CD95 stimulation, A-SMase is activated through translocation from intracellular compartments to the plasma membrane in an exocytic pathway requiring the t-SNARE protein syntaxin 4. Indeed, down-regulation of syntaxin 4 inhibits A-SMase translocation and activation induced by CD95 stimulation. This leads to inhibition of the CD95-triggered signaling events, including caspase 3 and 9 activation and apoptosis, activation of the survival pathway involving the protein kinase Akt, and important changes in cell cycle and proliferation. The molecular interaction between A-SMase and syntaxin 4 was not known and clarifies the mechanism of A-SMase activation. The novel actions of syntaxin 4 in sphingolipid metabolism and exocytosis we describe here define signaling mechanisms of broad relevance in cell pathophysiology. 相似文献
64.
Assi Weber 《Plant and Soil》1986,96(2):205-213
Summary The distribution of spore positive (Sp+) and spore negative (Sp−) nodules on the two native alder species (A. incana andA. glutinosa) in Finland was investigated. Nodules were collected throughout the country from different ecosystems (forests, swamps, lake-
sea- and riversides, old pastures and fields as well as from alder plantations).
OnA. incana Sp+ nodules predominated, whereas onA. glutinosa the vast majority of the nodules were of the Sp− type. Sp+ nodules onA. glutinosa were found only at sites where the two alder species grew close together. This distribution pattern indicates an association
of nodule type with alder species, the reasons for which are discussed. Indications of saprophytic growth in the Sp− strain were also found. 相似文献
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66.
P. Gourlay A. Decors M. Moinet O. Lambert B. Lawson F. Beaudeau S. Assié 《European Journal of Wildlife Research》2014,60(6):865-873
Multiple schemes for wildlife disease surveillance have been in operation in France for decades and data on wild bird carcasses presented to the national SAGIR network have been recorded since the 1980s. Over the same period, wildlife rescue centres (WRCs) have admitted thousands of birds each year. However, the reasons for casualty submission have been poorly explored to date. To assess the potential capacity of WRCs to monitor infectious and non-infectious diseases of wild birds in addition to SAGIR, we used Fringillidae and Passeridae data from January 2004 to April 2013 from SAGIR and the WRC of Nantes (CVFSE/Oniris) which is in operation in North-West France. Firstly, the Centre Vétérinaire de la Faune Sauvage et des Ecosystèmes des Pays de la Loire (CVFSE) contributed more than 30 % of all the birds submitted and was complementary to the SAGIR network in terms of species, age of the birds collected, location and date found. Secondly, the CVFSE was able to detect the emergent finch trichomonosis, in addition to the SAGIR network. Some causes of passerine submission were detected by one or other of the two schemes leading to their complementarity in overviewing Fringillidae and Passeridae infectious and non-infectious diseases in France. In order to improve the efficiency of its wild bird disease monitoring and to participate in an effective national and/or European surveillance network, the CVFSE, as for other WRCs, must enhance its diagnostic capabilities, in particular post-mortem examinations and laboratory testing. 相似文献
67.
The clayed filling of an holocene paleolagoon, from ?25 to ?11 m and dating from 8630 B.P. to 4300 B.P., is rich in fossil leaves of trees of swamp forest. It also contains some molluscan shells of a brackish environment. An attempt of palaeoecological reconstitution is made. 相似文献