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11.
Turhan A Tsuda A Konerding MA Lin M Miele L Lee G Mentzer SJ 《In vitro cellular & developmental biology. Animal》2008,44(10):426-433
A central feature of intussusceptive angiogenesis is the development of an intravascular pillar that bridges the opposing
sides of the microvessel lumen. In this report, we created polydimethyl siloxane (PDMS) microchannels with geometric proportions
based on corrosion casts of the colon microcirculation. The structure of the PDMS microchannels was a bifurcated channel with
an intraluminal pillar in the geometric center of the bifurcation. The effect of the intraluminal pillar on particle flow
paths was investigated using an in vitro perfusion system. The microchannels were perfused with fluorescent particles, and
the particle movements were recorded using fluorescence videomicroscopy. We found that the presence of an intravascular pillar
significantly decreased particle velocity in the bifurcation system (p < 0.05). In addition, the pillar altered the trajectory of particles in the center line of the flow stream. The particle
trajectory resulted in prolonged pillar contact as well as increased residence time within the bifurcation system (p < 0.001). Our results suggest that the intravascular pillar not only provides a mechanism of increasing resistance to blood
flow but may also participate in spatial redistribution of cells within the flow stream.
Supported in part by NIH Grants HL47078, HL75426, HL054885, HL070542 and HLO74022. 相似文献
12.
Effects of brassinosteroids on barley root growth,antioxidant system and cell division 总被引:1,自引:0,他引:1
Gonul Kartal Aslihan Temel Ercan Arican Nermin Gozukirmizi 《Plant Growth Regulation》2009,58(3):261-267
Homobrassinolide (HBR), which is one of the most biologically active forms of Brassinosteroids (BRs), was used to examine
the potential effects of hormone on root germination, antioxidant system enzymes and cell division of barley (Hordeum vulgare L.). Seeds were germinated between filter papers in 0.1, 0.5 and 1.0 μM HBR-supplemented distilled water for 48 h at dark
with their controls. HBR application increased especially the primary root growth significantly with increasing concentrations
when compared with the control materials and reached two fold increase in 1.0 μM HBR treated material. Treated and untreated
control group roots were fixed in 1:3 aceto-alcohol and aceto-orcein preparations were made. Roots treated with HBR showed
more mitotic activity, mitotic abnormalities and significant enlargements at the root tips when compared with control material.
HBR application decreased total soluble protein content, superoxide dismutase (EC 1.15.1.1), catalase (EC 1.11.1.6) and peroxidase
(EC 1.11.1.11) activities significantly at 1.0 μM HBR concentration. Data presented here is one of the first detailed analyses
of HBR effect on barley root development. 相似文献
13.
Familial Mediterranean Fever (FMF) is an autosomal recessive autoinflammatory disorder with the responsible gene of MEFV which
primarily affects Jewish, Armenian, Turkish and Arab populations. The FMF gene (MEFV) has recently been cloned to chromosome
16p, which encodes pyrin. In the present study, we enrolled 2,067 unrelated patients with the suspicion of FMF in Middle Anatolia
between the years 2006–2009 and identified the 12 MEFV mutations. DNA was amplified by PCR and subjected to reverse hybridization
for the detection of MEFV gene mutations. Among the 2,067 patients, 866 (41.9%) were males and 1,201 (58.1%) were females.
The mutations were homozygous in 176 (16.85%) patients, compound heterozygous in 314 (30.1%) patients, heterozygous in 546
(52.25%) patients and the other forms of mutations were found in 8 patients (0.76%). No mutation was detected in 1,023 (49.5%)
patients. The most frequent mutations were M694V, M680I (G/C), E148Q and V726A. We could not find any significant differences
between the two common mutations according to the gender. The high incidence of MEFV gene mutations in the Turkish population
indicated that newborn screening may be discussed in the future. Because of the ethnic origin of Anatolia, larger serial analyses
are necessary to investigate the rate and coexistence of these mutations. 相似文献
14.
Tanner NA Tolun G Loparo JJ Jergic S Griffith JD Dixon NE van Oijen AM 《The EMBO journal》2011,30(9):1830-1840
During DNA replication, repetitive synthesis of discrete Okazaki fragments requires mechanisms that guarantee DNA polymerase, clamp, and primase proteins are present for every cycle. In Escherichia coli, this process proceeds through transfer of the lagging-strand polymerase from the β sliding clamp left at a completed Okazaki fragment to a clamp assembled on a new RNA primer. These lagging-strand clamps are thought to be bound by the replisome from solution and loaded a new for every fragment. Here, we discuss a surprising, alternative lagging-strand synthesis mechanism: efficient replication in the absence of any clamps other than those assembled with the replisome. Using single-molecule experiments, we show that replication complexes pre-assembled on DNA support synthesis of multiple Okazaki fragments in the absence of excess β clamps. The processivity of these replisomes, but not the number of synthesized Okazaki fragments, is dependent on the frequency of RNA-primer synthesis. These results broaden our understanding of lagging-strand synthesis and emphasize the stability of the replisome to continue synthesis without new clamps. 相似文献
15.
16.
Trace elements are known to have a key role in myocardial metabolism. The accumulation (cobalt, arsenic, copper) or deficiency
(selenium, zinc) of trace elements may be responsible for idiopathic dilated cardiomyopathy. We investigated the trace element
concentrations (Cu, Zn, Mg) in sera from patients with dilated cardiomyopathy by atomic absorption spectrophotometry. We observed
that patients with dilated cardiomyopathies have higher copper and lower zinc concentrations in serum than healthy controls.
The magnesium concentrations of patients did not differ significantly from that of control subjects. 相似文献
17.
Localization of an Ataxia-Telangiectasia Gene to an −500-kb Interval on Chromosome 11q23.1: Linkage Analysis of 176 Families by an International Consortium 下载免费PDF全文
Ethan Lange Anna-Lise Borresen Xiaoguang Chen Luciana Chessa Sujata Chiplunkar Patrick Concannon Sugandha Dandekar Steven Gerken Kenneth Lange Teresa Liang Carmel McConville Jeff Polakow Oscar Porras Galit Rotman Ozden Sanal Sepideh Sheikhavandi Yosef Shiloh Eric Sobel Malcolm Taylor Milhan Telatar Sharon Teraoka Aslihan Tolun Nitin Udar Nancy Uhrhammer Lina Vanagaite Zhijun Wang Beth Wapelhorst Jocyndra Wright Huan-Ming Yang Lan Yang Yael Ziv Richard A. Gatti 《American journal of human genetics》1995,57(1):112-119
We describe a 20-point linkage analysis map of chromosome 11q22-23 that is based on genotyping 249 families (59 CEPH and 190 A-T). Monte Carlo linkage analyses of 176 ataxia-telangiectasia (A-T) families localizes the major A-T locus to the region between S1819(A4) and S1818(A2). When seven nonlinking families were excluded from subsequent analyses, a 2-lod support interval of ~500 kb was identified between S1819(A4) and S1294. No recombinants were observed between A-T and markers S384, B7, S535, or S1294. Only 17 of the international consortium families have been assigned to complementation groups. The available evidence favors either a cluster of A-T genes on chromosome 11 or intragenic defects in a single gene. 相似文献
18.
19.
M S Ahmed L W Randall B Sibai C Dass G Fridland D M Desiderio E Tolun 《Life sciences》1987,40(21):2067-2076
The human placental villus tissue contains opioid receptors and peptides. The opioid peptides extracted from the villus tissue were fractionated using reverse-phase high performance liquid chromatography and a radio-receptor assay. The presence of dynorphin 1-8 was corroborated by mass spectrometric production of (M + H) ion in the fast atom bombardment mode. This octa-peptide could be the natural ligand of the kappa opioid receptors present in the human placental villus tissue. 相似文献
20.
Localization of an ataxia-telangiectasia locus to a 3-cM interval on chromosome 11q23: Linkage analysis of 111 families by an international consortium 总被引:7,自引:6,他引:1 下载免费PDF全文
T. Foroud S. Wei Y. Ziv E. Sobel E. Lange A. Chao T. Goradia Y. Huo A. Tolun L. Chessa P. Charmley O. Sanal N. Salman C. Julier P. Concannon C. McConville A. M. R. Taylor Y. Shiloh K. Lange R. A. Gatti 《American journal of human genetics》1991,49(6):1263-1279
Linkage of at least two complementation groups of ataxia-telangiectasia (AT) to the chromosomal region 11q23 is now well established. We provide here an 18-point map of the surrounding genomic region, derived from linkage analysis of 40 CEPH families. On the basis of this map, 111 AT families from Turkey, Israel, England, Italy, and the United States were analyzed, localizing the AT gene(s) to an 8-cM sex-averaged interval between the markers STMY and D11S132/NCAM. A new Monte Carlo method for computing approximate location scores estimates this location as being at least 10(8) times more likely than the next most likely interval, with a support interval midway between STMY and D11S132 that is either 5.2 cM (sex-averaged and conservatively based on 3 lod scores from the maximum-location score) or 2.8 cM (male specific, based on a 2.72:1 interval-specific female-to-male distance ratio. 相似文献