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81.
Summary A new general method has been developed for the specific histochemical identification ofO-acyl sugars in any epithelial glycoprotein. These sugars include hexose, 6-deoxyhexose andN-acetylhexosamine with an ester substituenent(s) located on a potentialvicinal diol(s). In the procedure reported [the periodic acid-borohydride reduction-saponification-selective periodate oxidation-borohydride reduction-periodic acid-Schiff (PA-Bh-KOH-PA-Bh*-Bh-PAS) method] the initial PA-Bh treatment rendersvicinal diols located on either sialic acid or neutral sugars PAS unreactive. In the subsequent steps ester substituents are removed from bothO-acyl sugars andO-acyl sialic acids by saponification (KOH), sialic acidvicinal diols are selectively removed by the PA*-Bh sequence andO-acyl sugars are stained with the PAS technique. This method has the advantage that the results are obtained with a single section and the results are either positive or negative. Consequently, it is superior to the three indirect methods investigated because it does not require an observer to compare the intensity or the shade of the staining obtained with serial sections.Using the PA-Bh-KOH-PA*-Bh-PAS method we have demonstrated, for the first time, thatO-acyl sugars occur in the epithelial goblet cell glycoproteins of adult human colon. The effect of the presence ofO-acyl sugars on the interpretation of a number of other methods for the histochemical investigation of glycoproteins is discussed. It is recommended that the results obtained with the PA-Bh-KOH-PA*-Bh-PAS method be evaluated before histochemical procedures for the investigation of neutral sugars andO-acyl sialic acids are selected. 相似文献
82.
Ohne Zusammenfassung 相似文献
83.
The comparative performance of vegetative propagules (VP) grafted on ‘M. 9’ rootstock and own-rooted (OR) seedlings of the
same genotypes was investigated for apple [Malus x domestica (Borkh.)] fruit quality traits using offspring from 22 control-pollinated families. Fifteen fruit quality traits including
fruit size and shape, skin colour, russet and sensory eating characteristics were evaluated for individual seedlings. Estimates
of genetic variance–covariance matrices (G) were obtained and compared between the two treatments (OR versus VP) using Mantel’s test and regression approaches. Empirical
estimates of correlations, at the individual-tree and family-mean levels, between the two treatments were compared with those
obtained from deterministic simulations. Estimates of narrow-sense heritability (h
2) were higher in the VP population than in the OR population for 11 of the 15 traits. The estimated G matrices were significantly different between the two treatments. Simulation study showed that with an increase in the total
genetic variance, the correspondence between the two treatments at the family-mean level improved dramatically for different
family sizes. As the ratio of dominance/additive variance increased from 0 to 1, the family-mean correlations between the
two treatments decreased for all family sizes. The average estimated correlations between the two treatments at the family-mean
level were higher than at the individual-tree level (0.78 and 0.42, respectively). These observed correlations were very similar
to our theoretical expectations. In the light of these results, caution is required when comparing apple seedlings tested
on their own roots with those tested on ‘M. 9’ rootstock, as potential cultivar breeding parents. 相似文献
84.
Andrea Behrenswerth Nicole Volz Jakob Toräng Sonja Hinz Stefan Bräse Christa E. Müller 《Bioorganic & medicinal chemistry》2009,17(7):2842-2851
In the present study we synthesized 36 coumarin and 2H-chromene derivatives applying a recently developed umpoled domino reaction using substituted salicylaldehyde and α,β-unsaturated aldehyde derivatives as starting compounds. In radioligand binding studies 5-substituted 3-benzylcoumarin derivatives showed affinity to cannabinoid CB1 and CB2 receptors and were identified as new lead structures. In further GTPγS binding studies selected compounds were shown to be antagonists or inverse agonists. 相似文献
85.
The biological effects of tumor necrosis factor (TNF) include the enhancement of fibroblast proliferation, the secretion of collagenase and prostaglandin E2 (PGE2) by fibroblasts, and the resorption of bone and cartilage, suggesting a role for this cytokine in arthritic conditions. To investigate this, we measured the levels of TNF in synovial fluids and evaluated its secretion by synovial fluid mononuclear cells and tissues from patients with rheumatoid arthritis, osteoarthritis, and seronegative arthritis and normals. TNF was found to be secreted in all arthritic conditions but not in normals. The levels of TNF were highest in synovial fluid and correlated with interferon-gamma (IFN-gamma) levels but not PGE2. The production of TNF was stable in a single joint for 3 to 6 months. Using immunohistochemical staining, TNF was localized to mononuclear cells in the lining layer, sublining, and perivascular areas of synovial tissue. The secretion of TNF by rheumatoid synovial fluid mononuclear cells was inhibited by PGE2, while IFN-gamma enhanced its production in those cells which were spontaneously secreting TNF. Our data suggest that TNF may play a role in various arthritic diseases. 相似文献
86.
Crystal structure of Escherichia coli CheY refined at 1.7-A resolution 总被引:19,自引:0,他引:19
The three-dimensional structure of wild-type CheY from Escherichia coli has been refined by stereochemically restrained least squares minimization to a crystallographic R-factor of 15.1% at 1.7-A resolution. The structure contains 1165 atoms, including all atoms of the protein, 147 water molecules, and three sulfate ions. The final model has root mean square deviations of 0.018 and 0.049 A from idealized bond lengths and angle distances, respectively. Seven amino acid side chains have been modeled in dual conformations. CheY folds as a compact (beta/alpha)5 globular protein, with the phosphorylation region contained in a cavity on one face of the molecule. This active site area is bordered by the carboxyl termini of the three central beta-strands, by alpha 1, and by the loop connecting beta 5 to alpha 5. The Lys-109 side chain of this loop extends into the active site by virtue of its cis peptide bond conformation preceding Pro-110. The epsilon-amino group of Lys-109 is in close bonding contact with the carboxyl group of Asp-57, the residue that is phosphorylated in the activation process of CheY. The details of the hydrogen bonding network in the phosphorylation region indicate that structural rearrangements must accompany the phosphorylation of Asp-57. 相似文献
87.
88.
Synaptinemal complexes in Schizophyllum commune. 总被引:1,自引:0,他引:1
89.
Multiple Repeats of a Promoter Segment Causes Transcription Factor Autoregulation in Red Apples 总被引:2,自引:0,他引:2
90.