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151.
This paper presents the results of an investigation on the distribution of210Po in Mutharasanallur pond ecosystem. It has been demonstrated that210Po is non-uniformly distributed within the ecosystem. The results of the study show a dissolved210Po concentration in pond water of 1 4mBq 1−1. The sediment samplso recorded a210Po activity of 59 9 Bq kg−1. The aquatic organisms showed differential accumulation of the radionuclide with enhanced bioaccumulation in soft tissues
and muscle. The210Po activity in the biota fell within the range of 1·2–53 3 Bq kg−1 (wet wt). The bivalve mussel,Lamellidens marginalis was identified to accumulate higher concentration of210Poin soft tissues, suggesting that these organisms could serve as a bio-monitor of210Po radionuclide in a freshwater system. The concentration factors of210Po for the biotic components ranged from ∼102–∼104. Analyses of the results indicate that prawn and fish represent an important source of supply of210Po to humans via dietary intake. Results of210Po activity in the abiotic and biotic components of the pond ecosystem were higher when compared with those of Cauvery river
system, the primary water source of the pond. 相似文献
152.
Bacillus subtilis, isolated from tannery waste, produced an alkaline protease at optimal activity when grown in a casein/gelatine medium in a stirred tank fermenter at 37°C with the dissolved oxygen tension at 40% air saturation. Optimum protease activity (223 U ml-1) was at pH 8.5 and was stable for 1 h up to 45°C but at 60°C lost 80% activity. Use of the crude protease as a bating agent for producing high quality leather is indicated. Tensile strength, bursting strength, tear strength and elongation at break of prepared leather were increased with increasing amounts of protease used for bating.A. Hameed and M.A. Natt are with the Department of Biological Sciences, Quaid-i-Azam University, Islamabad. Pakistan. C.S. Evans is with the School of Biological and Health Sciences, University of Westminster, London. UK. 相似文献
153.
Umul Kulthum Ahmed N. Claire Maller Asif J. Iqbal Lamyaa Al-Riyami William Harnett John G. Raynes 《The Journal of biological chemistry》2016,291(22):11939-11953
Parasitic nematodes manufacture various carbohydrate-linked phosphorylcholine (PCh)-containing molecules, including ES-62, a protein with an N-linked glycan terminally substituted with PCh. The PCh component is biologically important because it is required for immunomodulatory effects. We showed that most ES-62 was bound to a single protein, C-reactive protein (CRP), in normal human serum, displaying a calcium-dependent, high-avidity interaction and ability to form large complexes. Unexpectedly, CRP binding to ES-62 failed to efficiently activate complement as far as the C3 convertase stage in comparison with PCh-BSA and PCh-containing Streptococcus pneumoniae cell wall polysaccharide. C1q capture assays demonstrated an ES-62-CRP-C1q interaction in serum. The three ligands all activated C1 and generated C4b to similar extents. However, a C2a active site was not generated following ES-62 binding to CRP, demonstrating that C2 cleavage was far less efficient for ES-62-containing complexes. We proposed that failure of C2 cleavage was due to the flexible nature of carbohydrate-bound PCh and that reduced proximity of the C1 complex was the reason that C2 was poorly cleaved. This was confirmed using synthetic analogues that were similar to ES-62 only in respect of having a flexible PCh. Furthermore, ES-62 was shown to deplete early complement components, such as the rate-limiting C4, following CRP interaction and thereby inhibit classical pathway activation. Thus, flexible PCh-glycan represents a novel mechanism for subversion of complement activation. These data illustrate the importance of the rate-limiting C4/C2 stage of complement activation and reveal a new addition to the repertoire of ES-62 immunomodulatory mechanisms with possible therapeutic applications. 相似文献
154.
Seeds with efficient antioxidant defence system show higher germination under stress conditions; however, such information is limited for the halophyte seeds. We therefore studied lipid peroxidation and antioxidant responses of a leaf-succulent halophyte Salsola drummondii during seed germination under different salinity levels (0, 200 and 800 mM NaCl), temperature (10/20, 20/30 and 25/35°C) and light regimes. Seeds absorbed water and germinated in less than 1 h in non-saline control while increases in salinity decreased the rate of water uptake as well as seed germination. Non-optimal temperatures (10/20 and 25/35°C) and complete dark condition reduced seed germination in comparison to those seeds germinated under optimal temperature (20/30°C) and 12-h photoperiod, respectively. Generally, higher lipid peroxidation and antioxidant enzyme activities were observed in seeds at non-optimal temperature and in those seeds germinated in dark. Decrease in reduced ascorbic acid content was found in highest salinity and temperature treatments, while reduced glutathione content did not change significantly with changes in salinity, temperature and light regimes. These results indicate variation in temperature and light but not salinity enhances antioxidant enzyme activities in germinating seeds of Salsola drummondii. 相似文献
155.
Rukhsana Gul Dae-Ryoung Park Asif Iqbal Shawl Soo-Yeul Im Tae-Sik Nam Sun-Hwa Lee Jae-Ki Ko Kyu Yoon Jang Donghee Kim Uh-Hyun Kim 《PloS one》2016,11(3)
Ca2+ signaling plays a fundamental role in cardiac hypertrophic remodeling, but the underlying mechanisms remain poorly understood. We investigated the role of Ca2+-mobilizing second messengers, NAADP and cADPR, in the cardiac hypertrophy induced by β-adrenergic stimulation by isoproterenol. Isoproterenol induced an initial Ca2+ transients followed by sustained Ca2+ rises. Inhibition of the cADPR pathway with 8-Br-cADPR abolished only the sustained Ca2+ increase, whereas inhibition of the NAADP pathway with bafilomycin-A1 abolished both rapid and sustained phases of the isoproterenol-mediated signal, indicating that the Ca2+ signal is mediated by a sequential action of NAADP and cADPR. The sequential production of NAADP and cADPR was confirmed biochemically. The isoproterenol-mediated Ca2+ increase and cADPR production, but not NAADP production, were markedly reduced in cardiomyocytes obtained from CD38 knockout mice. CD38 knockout mice were rescued from chronic isoproterenol infusion-induced myocardial hypertrophy, interstitial fibrosis, and decrease in fractional shortening and ejection fraction. Thus, our findings indicate that β-adrenergic stimulation contributes to the development of maladaptive cardiac hypertrophy via Ca2+ signaling mediated by NAADP-synthesizing enzyme and CD38 that produce NAADP and cADPR, respectively. 相似文献
156.
Jian Chen Gottumukkala S. Raju Wilma Jogunoori Vipin Menon Avijit Majumdar Jiun-Sheng Chen Young Jin Gi Yun Seong Jeong Liem Phan Mitchell Belkin Shoujun Gu Suchin Kundra Nipun A. Mistry Jianping Zhang Xiaoping Su Shulin Li Sue-Hwa Lin Milind Javle John S. McMurray Thomas F. Rahlfs Bibhuti Mishra Jon White Asif Rashid Nicole Beauchemin Brian R. Weston Mehnaz A. Shafi John R. Stroehlein Marta Davila Rehan Akbani John N. Weinstein Xifeng Wu Lopa Mishra 《PloS one》2016,11(4)
157.
Intra and interstrain crosses were made between unselected and inbred adults of the Balloki and Peshawar, Pakistan, laboratory colonies of C. tritaeniorhynchus and life tables were constructed for their progeny. No overall heterosis effects were detected. Inbreeding severely reduced fertility and fecundity and lowered the innate capacity for increase. Interstrain crosses among inbred adults restored some vigor, but all life table estimates relating to fecundity were significantly less than crosses among the unselected adults. Thus, outcrosses among laboratory-adapted colonies did not appear to impart marked heterosis and would probably not enhance the vigor of males used for genetic control.
Zusammenfassung Intra- und Interstammkreuzungen wurden zwischen unselektionierten und ingezüchteten Insekten der Balloki- und Peshawar-Laboratoriumskolonien von C. tritaeniorhynchus durchgeführt und es wurden Life Tables für ihre Nachkommenschaft erstellt. Dabei konnten keine allgemeinen Heterosis-effekte festgestellt werden. Inzucht verminderte die Fertilität und Fekundität stark und reduzierte die genetische Vermehrungsfähigkeit. Interstammkreuzungen zwischen Inzuchtinsekten brachte etwas Vigor zurück, aber alle Life Table-Schätzungen im Hinblick auf Fekundität waren gesichert tiefer als bei Kreuzungen zwischen nicht selektionierten Insekten. Demnach scheinen Kreuzungen zwischen verschiedenen dem Laboratorium angepassten Kolonien keine ausgeprägte Heterosis zu bringen. Sie würden wahrscheinlich die Qualität von Männchen und Weibchen, die für die genetische Bekämpfung verwendet werden, nicht verbessern.相似文献
159.
160.
Mariana Costa Braga Schmidt Katia L.P. Morais Maíra Estanislau Soares de Almeida Asif Iqbal Mauricio Barbugiani Goldfeder 《Cell Adhesion & Migration》2020,14(1):129-138
ABSTRACT
In a tumor microenvironment, endothelial cell migration and angiogenesis allow cancer to spread to other organs causing metastasis. Indeed, a number of molecules that are involved in cytoskeleton re-organization and intracellular signaling have been investigated for their effects on tumor cell growth and metastasis. Alongside that, Amblyomin-X, a recombinant Kunitz-type protein, has been shown to reduce metastasis and tumor growth in in vivo experiments. In the present report, we provide a mechanistic insight to these antitumor effects, this is, Amblyomin-X modulates Rho-GTPases and uPAR signaling, and reduces the release of MMPs, leading to disruption of the actin cytoskeleton and decreased cell migration of tumor cell lines. Altogether, our data support a role for Amblyomin-X as a novel potential antitumor drug. 相似文献