全文获取类型
收费全文 | 591篇 |
免费 | 45篇 |
出版年
2022年 | 5篇 |
2021年 | 10篇 |
2020年 | 5篇 |
2019年 | 9篇 |
2018年 | 12篇 |
2017年 | 20篇 |
2016年 | 13篇 |
2015年 | 17篇 |
2014年 | 24篇 |
2013年 | 28篇 |
2012年 | 39篇 |
2011年 | 35篇 |
2010年 | 19篇 |
2009年 | 18篇 |
2008年 | 30篇 |
2007年 | 30篇 |
2006年 | 37篇 |
2005年 | 28篇 |
2004年 | 36篇 |
2003年 | 22篇 |
2002年 | 26篇 |
2001年 | 12篇 |
2000年 | 15篇 |
1999年 | 8篇 |
1998年 | 4篇 |
1997年 | 5篇 |
1996年 | 5篇 |
1995年 | 3篇 |
1992年 | 7篇 |
1991年 | 5篇 |
1990年 | 4篇 |
1988年 | 4篇 |
1987年 | 5篇 |
1986年 | 5篇 |
1985年 | 8篇 |
1984年 | 7篇 |
1982年 | 3篇 |
1981年 | 3篇 |
1980年 | 9篇 |
1979年 | 4篇 |
1978年 | 6篇 |
1977年 | 5篇 |
1976年 | 3篇 |
1975年 | 4篇 |
1973年 | 9篇 |
1972年 | 3篇 |
1970年 | 3篇 |
1969年 | 3篇 |
1968年 | 5篇 |
1967年 | 4篇 |
排序方式: 共有636条查询结果,搜索用时 15 毫秒
631.
632.
Li-Hua Peng Xiao Liang Rui-Heng Chang Jia-Yi Mu Hui-E Chen Asami Yoshida 《Biofouling》2020,36(7):753-765
AbstractLarval settlement and metamorphosis is essential for the development of marine invertebrates. Although polysaccharides are involved in larval settlement and metamorphosis of Mytilus coruscus, the molecular basis of polysaccharides underlying this progression remains largely unknown. Here, the roles of the polysaccharide biosynthesis-related gene 01912 of Pseudoalteromonas marina ECSMB14103 in the regulation of larval settlement and metamorphosis were examined by gene-knockout technique. Compared with biofilms (BFs) of the wild-type P. marina, Δ01912 BFs with a higher colanic acid (CA) content showed a higher inducing activity on larval settlement and metamorphosis. Deletion of the 01912 gene caused an increase in c-di-GMP levels, accompanied by a decrease in the motility, an increase in cell aggregation, and overproduction of CA. Thus, the bacterial polysaccharide biosynthesis-related gene 01912 may regulate mussel settlement by producing CA via the coordination of c-di-GMP. This work provides a deeper insight into the molecular mechanism of polysaccharides in modulating mussel settlement. 相似文献
633.
634.
Studies on pyrocatechase. I. Purification and spectral properties 总被引:16,自引:0,他引:16
Y Kojima H Fujisawa A Nakazawa T Nakazawa F Kanetsuna H Taniuchi M Nozaki O Hayaishi 《The Journal of biological chemistry》1967,242(14):3270-3278
635.
636.
Amide-hydrogen exchange of three anti-yeast iso-1-cytochrome-c IgG monoclonal antibodies and the Fab, prepared from one of them, were studied by infrared spectrophotometry in the presence and absence of the deuterated immunogen and evolutionarily related species (the deuterated immunogen contained a population of a dimer. Each subunit of the dimer appeared to bind to the antibodies in a manner similar to the monomer). The number of hydrogens of the antibodies whose exchange was suppressed on binding to the immunogen was found to exceed that estimated for the residues shielded by the immunogen. Analysis of the data suggests that such suppression of hydrogen exchange occurs mainly for the Fab domains, but not for the Fc. One of the antibodies showed two distinct classes of amide-hydrogens. Class-1 hydrogens (approx. 36/site) exchange faster than class 2 (approx. 37/site). The exchange of class-1 hydrogens was suppressed by binding to the immunogen, but not to the evolutionarily related species. The exchange of class-2 hydrogens was suppressed by binding to the evolutionarily related species, as well as to the immunogen. Thus, the suppression of exchange of class-1 hydrogens appears to occur by some kind of conformational stabilization, the mechanism of which differentiates between the deuterated immunogen and the evolutionarily related species. Evidence suggests that the trans-interactions of the Fab domains may modulate the hydrogen exchange. If it is assumed that the antigen-binding strengthens the trans-interactions in such a way that the exchange of the slower exchanging hydrogens is suppressed, this could explain the suppression of exchange of class-2 hydrogens. 相似文献