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551.
552.
Daichi Watanabe Ikuo Takahashi Naiyanate Jaroensanti-Tanaka Sho Miyazaki Kai Jiang Masaru Nakayasu Masato Wada Tadao Asami Masaharu Mizutani Kazuma Okada Masatoshi Nakajima 《The Plant journal : for cell and molecular biology》2021,105(4):1026-1034
Ectopic expression of the apple 2-oxoglutarate-dependent dioxygenase (DOX, 2ODD) gene, designated MdDOX-Co, is thought to cause the columnar shape of apple trees. However, the mechanism underlying the formation of such a unique tree shape remains unclear. To solve this problem, we demonstrated that Arabidopsis thaliana overexpressing MdDOX-Co contained reduced levels of biologically active gibberellin (GA) compared with wild type. In summary: (i) with biochemical approaches, the gene product MdDOX-Co was shown to metabolize active GA A4 (GA4) to GA58 (12-OH-GA4) in vitro. MdDOX-Co also metabolized its precursors GA12 and GA9 to GA111 (12-OH-GA12) and GA70 (12-OH-GA9), respectively; (ii) Of the three 12-OH-GAs, GA58 was still active physiologically, but not GA70 or GA111; (iii) Arabidopsis MdDOX-Co OE transformants converted exogenously applied deuterium-labeled (d2)-GA12 to d2-GA111 but not to d2-GA58, whereas transformants converted applied d2-GA9 to d2-GA58; (iv) GA111 is converted poorly to GA70 by GA 20-oxidases in vitro when GA12 is efficiently metabolized to GA9; (v) no GA58 was detected endogenously in MdDOX-Co OE transformants. Overall, we conclude that 12-hydroxylation of GA12 by MdDOX-Co prevents the biosynthesis of biologically active GAs in planta, resulting in columnar phenotypes. 相似文献
553.
T Kajino Y Saito O Asami Y Yamada M Hirai S Udata 《Journal of industrial microbiology & biotechnology》1997,19(4):227-231
The characteristic features of the Bacillus brevis system are very high productivity of heterologous proteins and very low extracellular protease activity. However, degradation
of some heterologous proteins, especially mammalian proteins, can be observed and resulted in a lowering of protein productivity.
By using a mutant expressing low levels of proteases and the addition of EDTA to the medium, intact human growth hormone
(hGH) was successfully produced with the B. brevis system. Signal peptide modification with higher basicity in the amino terminal region and higher hydrophobicity in the middle
region brought about a twelve-fold increase in hGH production. The hGH yield was further elevated to 240 mg L−1 by optimization of culture conditions. Thus, biologically active and mature hGH can be efficiently produced directly in
the medium with the B. brevis system.
Received 06 March 1997/ Accepted in revised form 03 July 1997 相似文献
554.
Asami Tadao; Robertson Masumi; Yamamoto Shin; Yoneyama Koichi; Takeuchi Yasutomo; Yoshida Shigeo 《Plant & cell physiology》1998,39(3):342-348
Biological activities of an abscisic acid (ABA) analog, RCA-7a[l-(3-carboxyl-5-methylphenyl)-l-hydroxy-2,6,6-trimethyl-4-oxo-2-cyclohexene],carrying a phenyl group in the side chain are reported. ( +)-RCA-7a was approximately 3-fold less active than ( + )-ABAin the inhibition of 相似文献
555.
Shiro Someya Kenjiro Koyama Nozomu Asami Tetsuo Kataoka Yuichi Yamamura 《Microbiology and immunology》1966,10(4):221-227
A comparative study of the tuberculin potency and reactivity to TAP and PPD-S was made in humans and guinea pigs. A comparison of reactions elicited by TAP and PPD-S was made in two groups of guinea pigs previously inoculated with killed bacilli or with living bacilli. The tuberculin reactions produced by TAP and PPD-S in human beings was studied in school children from rural districts. These children were divided randomly into three groups and each group was given a different amount of TAP and PPD-S. The results obtained are as follows: (1) While relative potency of TAP was relatively much weaker than PPD-S in guinea pigs sensitized with tubercle bacilli, in human beings the tuberculin potency of TAP was approximately 1/2 or 1/3 that of PPD-S. (2) There was a marked difference in the tuberculin reactivity to TAP and PPD-S between guinea pigs immunized with killed bacilli and guinea pigs immunized with living bacilli. 相似文献
556.
Gregorio Galvez-Valdivieso Michael J. Fryer Tracy Lawson Katie Slattery William Truman Nicholas Smirnoff Tadao Asami William J. Davies Alan M. Jones Neil R. Baker Philip M. Mullineaux 《The Plant cell》2009,21(7):2143-2162
Previously, it has been shown that Arabidopsis thaliana leaves exposed to high light accumulate hydrogen peroxide (H2O2) in bundle sheath cell (BSC) chloroplasts as part of a retrograde signaling network that induces ASCORBATE PEROXIDASE2 (APX2). Abscisic acid (ABA) signaling has been postulated to be involved in this network. To investigate the proposed role of ABA, a combination of physiological, pharmacological, bioinformatic, and molecular genetic approaches was used. ABA biosynthesis is initiated in vascular parenchyma and activates a signaling network in neighboring BSCs. This signaling network includes the Gα subunit of the heterotrimeric G protein complex, the OPEN STOMATA1 protein kinase, and extracellular H2O2, which together coordinate with a redox-retrograde signal from BSC chloroplasts to activate APX2 expression. High light–responsive genes expressed in other leaf tissues are subject to a coordination of chloroplast retrograde signaling and transcellular signaling activated by ABA synthesized in vascular cells. ABA is necessary for the successful adjustment of the leaf to repeated episodes of high light. This process involves maintenance of photochemical quenching, which is required for dissipation of excess excitation energy. 相似文献
557.
Li-Hua Peng Xiao Liang Rui-Heng Chang Jia-Yi Mu Hui-E Chen Asami Yoshida 《Biofouling》2020,36(7):753-765
AbstractLarval settlement and metamorphosis is essential for the development of marine invertebrates. Although polysaccharides are involved in larval settlement and metamorphosis of Mytilus coruscus, the molecular basis of polysaccharides underlying this progression remains largely unknown. Here, the roles of the polysaccharide biosynthesis-related gene 01912 of Pseudoalteromonas marina ECSMB14103 in the regulation of larval settlement and metamorphosis were examined by gene-knockout technique. Compared with biofilms (BFs) of the wild-type P. marina, Δ01912 BFs with a higher colanic acid (CA) content showed a higher inducing activity on larval settlement and metamorphosis. Deletion of the 01912 gene caused an increase in c-di-GMP levels, accompanied by a decrease in the motility, an increase in cell aggregation, and overproduction of CA. Thus, the bacterial polysaccharide biosynthesis-related gene 01912 may regulate mussel settlement by producing CA via the coordination of c-di-GMP. This work provides a deeper insight into the molecular mechanism of polysaccharides in modulating mussel settlement. 相似文献