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991.
992.
Ann Margaret Grøndahl Ellen Margrete Skancke Cecilie Marie Mejdell Johan Høgset Jansen 《Acta veterinaria Scandinavica》2007,49(1):16
Over a period of two years, growth rate and health were measured for dairy calves allowed to suckle their mothers up to 6–8
weeks of age. Thirty-one calves were weighted weekly, and the mean daily growth rate was 1.2 ± 0.03 kg from birth up to 13
weeks of age. Illness in calves and young stock was not observed. In the cows, the mean incidences of ketosis, displaced abomasum,
puerperal paresis, mastitis, teat injury and retained placenta were 0, 0, 8, 22, 1 and 1%, respectively, during a 6-year period.
The mean daily gain of 56 growing bulls was 1.4 kg when slaughtered at 15 months of age, which is higher than the mean daily
gain of 0.95 kg in the population. Probiotics, hormones and vaccines were not used, and antibiotics were only used for treating
illness. The present study indicates many advantages and few problems when dairy calves are penned together with the cows
and allowed natural feeding up to 6–8 weeks of age. This production system was easy to manage, preferred by the farmer, and
may satisfy the public concern regarding the practice of immediate separation of cow and calf in commercial milk production. 相似文献
993.
Mycoplasma alkalescens is an arginine-metabolizing mycoplasma, which has been found in association with mastitis and arthritis in cattle. Routine
bacteriological examination of 17 bronchoalveolar lavage samples from calves with pneumonia in a single herd in Denmark, identified
M. alkalescens in eight samples. The organism was found as a sole bacterilogical findings in five of the samples as well as in combination
with Mannheimia haemolytica, Haemophilus somni and Salmonella Dublin. This is the first report of isolation of M. alkalescens in Denmark. 相似文献
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999.
Beginning in the early 1990s, the balsam fir sawfly (Neodiprion abietis) became a significant defoliating insect of precommercially thinned balsam fir (Abies balsamea (L.) Mill.) stands in western Newfoundland, Canada. In 1997, a nucleopolyhedrovirus (NeabNPV) was isolated from the balsam
fir sawfly and, as no control measures were then available, NeabNPV was developed for the biological control of balsam fir
sawfly. In order to register NeabNPV for operational use under the Canadian Pest Control Products Act, research was carried
out in a number of areas including NeabNPV field efficacy, non-target organism toxicology, balsam fir sawfly ecology and impact
on balsam fir trees, and NeabNPV genome sequencing and analysis. As part of the field efficacy trials, approximately 22 500
hectares of balsam fir sawfly-infested forest were aerially treated with NeabNPV between 2000 and 2005. NeabNPV was found
to be safe, efficacious, and economical for the suppression of balsam fir sawfly outbreak populations. Conditional registration
for the NeabNPV-based product, Abietiv™, was received from the Pest Management Regulatory Agency (Health Canada) in April
2006. In July 2006, Abietiv was applied by spray airplanes to 15 000 ha of balsam fir sawfly-infested forest in western Newfoundland
in an operational control program.
相似文献
1000.
To achieve higher level expression of Interferon α2b (IFN-α2b) in methylotrophic yeast (Pichia pastoris), a cDNA fragment coding for the mature IFN-α2b was designed and synthesized based on the synonymous codon bias of P. pastoris and optimized G+C content. The synthetic IFN-α2b was inserted into the secreted expression vector pPICZαA, and then integrated
into P. pastoris GS115 genome by electroporation. Multi-copy integrants in the Mut+ recombinant P. pastoris strain were screened by high concentrations of Zeocin. 120 hours culturing allowed expression of the IFN-α2b transformant
up to 810 mg/L as detected by SDS-PAGE and quantitative methods. In addition, Western blot analysis showed that the recombinant
proteins had immunogenicity. The significant antiviral activity of the recombinant IFN-α2b protein was verified by WISH/ VSV
system, which was 3.3×105 IU/mL.
Foundation items: The National ‘973’ Basic Research Program (2002CB111302); The National Natural Science Foundation of China
(30370807) 相似文献