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Paul Prajita Patel Paritosh Verma Suresh K. Mishra Pragyan Sahu Bikash R. Panda Pritam Kumar Kushwaha Gajraj Singh Senapati Shantibhusan Misra Namrata Suar Mrutyunjay 《Cell biology and toxicology》2022,38(1):111-127
Cell Biology and Toxicology - The key to bacterial virulence relies on an exquisite balance of signals between microbe and hosts. Bacterial toxin–antitoxin (TA) system is known to play a... 相似文献
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Diversity of some insect fauna in industrial and non-industrial areas of West Bengal, India 总被引:1,自引:0,他引:1
The present work is designed to study diversity of five insect orders (viz., Hemiptera, Orthoptera, aculeate Hymenoptera,
Lepidoptera and Coleoptera) in the industrial region of Haldia (India) and in non-industrial area of the same district and
to evaluate the impact of industrialization on the biodiversity of those insect orders. The objective also extended to find
out the possibility of existence of bioindicator, if any. Eight study sites were selected from the East part of Midnapur district
covering 40 km aerial distance. Out of eight different study sites, five were distributed in and around Haldia industrial
complex and three in industry-free area. During this study, a total of 120 species under 98 genera in 37 families of insects
were collected. Binary data of 5 orders revealed that the species richness of Hemiptera, Orthoptera and Lepidoptera is higher
in non-industrial zone in comparison to that of industrial zone. Aculeate Hymenoptera shows no particular trend whereas Coleoptera
shows higher species richness in industrial areas. Results of multivariate analyses are compared with the species richness
data for all the eight study sites. It is concluded that even in an apparently homogeneous ecological condition species richness
may drastically change with the influence of industries. Total insect fauna decline by at least 23.33% is noticed in industrial
areas. It is found that some species of lepidopteran, hemipteran and orthopteran insects are susceptible to industrial pollution
and some of the members of these orders may be considered as a bioindicator group. 相似文献
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Baglole CJ Bushinsky SM Garcia TM Kode A Rahman I Sime PJ Phipps RP 《American journal of physiology. Lung cellular and molecular physiology》2006,291(1):L19-L29
Cigarette smoke is the principal cause of emphysema. Recent attention has focused on the loss of alveolar fibroblasts in the development of emphysema. Fibroblasts may become damaged by oxidative stress and undergo apoptosis as a result of cigarette smoke exposure. Not all smokers develop lung diseases associated with tobacco smoke, a fact that may reflect individual variation among human fibroblast strains. We hypothesize that fibroblasts from different human beings vary in their ability to undergo apoptosis after cigarette smoke exposure. This could account for emphysematous changes that occur in the lungs of some but not all smokers. Primary human lung fibroblast strains were exposed to cigarette smoke extract (CSE) and assessed for viability, morphological changes, and mitochondrial transmembrane potential as indicators of apoptosis. We also examined the generation of intracellular reactive oxygen species (ROS), 4-hydroxy-2-nonenal, and changes in glutathione (GSH) and glutathione disulfide (GSSG) levels. Each human lung fibroblast strain exhibited a differential sensitivity to CSE as judged by changes in mitochondrial membrane potential, viability, ROS generation, and glutathione production. Interestingly, the thiol antioxidants N-acetyl-L-cysteine and GSH eliminated CSE-induced changes in fibroblast morphology such as membrane blebbing, nuclear condensation, and cell size and prevented alterations in mitochondrial membrane potential and the generation of ROS. These findings support the concept that oxidative stress and apoptosis are responsible for fibroblast death associated with exposure to tobacco smoke. Variations in the sensitivity of fibroblasts to cigarette smoke may account for the fact that only some smokers develop emphysema. 相似文献
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Chemical and enzymatic incorporation of N2-(p-n-butylphenyl)-2''-deoxyguanosine into an oligodeoxyribonucleotide. 下载免费PDF全文
An 18mer oligodeoxyribonucleotide containing a N2-(p-n-butylphenyl)-2'-deoxyguanosine (BuPdG) residue at the 3' end has been synthesized by both chemical and enzymatic methods. Chemical synthesis involved attachment of 5'-DMT-BuPdG as the 3'-H-phosphonate to uridine-controlled pore glass (CPG), followed by extension via H-phosphonate chemistry. After oxidation of the backbone, deprotection of bases, and removal from CPG, the uridine residue was removed by periodate cleavage and beta-elimination. The resulting oligomer 3'-phosphate was digested with alkaline phosphatase to give the free BuPdG-18mer. E.coli DNA polymerase I (Klenow) incorporated BuPdGTP at the 3' end of the corresponding 17mer primer annealed to a complementary 29mer template, and the properties of this product were identical to those of chemically synthesized BuPdG-18mer. E.coli DNA polymerase I (Klenow) was unable to extend the BuPdG-18mer, and the 3' to 5' exonuclease activity of the enzyme was unable to remove the modified nucleotide. 相似文献
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Bavro VN Pietras Z Furnham N Pérez-Cano L Fernández-Recio J Pei XY Misra R Luisi B 《Molecular cell》2008,30(1):114-121
Drugs and certain proteins are transported across the membranes of Gram-negative bacteria by energy-activated pumps. The outer membrane component of these pumps is a channel that opens from a sealed resting state during the transport process. We describe two crystal structures of the Escherichia coli outer membrane protein TolC in its partially open state. Opening is accompanied by the exposure of three shallow intraprotomer grooves in the TolC trimer, where our mutagenesis data identify a contact point with the periplasmic component of a drug efflux pump, AcrA. We suggest that the assembly of multidrug efflux pumps is accompanied by induced fit of TolC driven mainly by accommodation of the periplasmic component. 相似文献