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排序方式: 共有180条查询结果,搜索用时 31 毫秒
41.
A white rot fungus, identified as Trametes hirsuta based on morphological and phylogenetic analysis, was found to contain efficient cellulose degrading enzymes. The strain showed maximum endoglucanase (EG), cellobiohydrolase (CBH) and ß-glucosidase (BGL) activities of 55, 0.28 and 5.0 U/mg-protein, respectively. Rice straw was found to be a potentially good substrate for growth of T. hirsuta for cellulase production. Statistical experimental design was used to optimize hydrolysis parameters such as pH, temperature, and concentrations of substrates and enzymes to achieve the highest saccharification yield. Enzyme concentration was identified as the limiting factor for saccharification of rice straw. A maximum saccharification rate of 88% was obtained at an enzyme concentration of 37.5 FPU/g-substrate after optimization of the hydrolysis parameters. The results of a confirmation experiment under the optimum conditions agreed well with model predictions. T. hirsuta may be a good choice for the production of reducing sugars from cellulosic biomass. 相似文献
42.
Suk-Jin Ha Sang-Yong Kim Jin-Ho Seo Marimuthu Jeya Ye-Wang Zhang Thangadurai Ramu In-Won Kim Jung-Kul Lee 《Bioprocess and biosystems engineering》2009,32(5):697-700
Of various metal ions (Ca2+, Cr3+, Cu2+, Fe2+, Mg2+, Mn2+, Ni2+ and Zn2+) added to the culture medium of Agrobacterium tumefaciens at 1 mM, only Ca2+ increased Coenzyme Q10 (CoQ10) content in cells without the inhibition of cell growth. In a pH-stat fed-batch culture, supplementation with 40 mM of CaCO3 increased the specific CoQ10 content and oxidative stress by 22.4 and 48%, respectively. Also, the effect of Ca2+ on the increase of CoQ10 content was successfully verified in a pilot-scale (300 L) fermentor. In this study, the increased oxidative stress in A. tumefaciens culture by the supplementation of Ca2+ is hypothesized to stimulate the increase of specific CoQ10 content in order to protect the membrane against lipid peroxidation. Our results improve the understanding of Ca2+ effect on CoQ10 biosynthesis in A. tumefaciens and should contribute to better industrial production of CoQ10 by biological processes. 相似文献
43.
Marimuthu Jeya Ah-Reum Joo Kyoung-Mi Lee Won-Il Sim Deok-Kun Oh Yeong-Suk Kim In-Won Kim Jung-Kul Lee 《Applied microbiology and biotechnology》2010,85(4):1005-1014
A novel endo-β-1,4-glucanase (EG)-producing strain was isolated and identified as Penicillium pinophilum KMJ601 based on its morphology and internal transcribed spacer (ITS) rDNA gene sequence. When rice straw and corn steep powder
were used as carbon and nitrogen sources, respectively, the maximal EG activity of 5.0 U mg protein−1, one of the highest levels among EG-producing microorganisms, was observed. The optimum temperature and pH for EG production
were 28°C and 5.0, respectively. The increased production of EG by P. pinophilum in culture at 28°C was confirmed by two-dimensional electrophoresis followed by MS/MS sequencing of the partial peptide.
A partial EG gene (eng5) was amplified by degenerate polymerase chain reaction (PCR) based on the peptide sequence. A full-length eng5 was cloned by genome-walking PCR, and P. pinophilum EG was identified as a member of glycoside hydrolase family 5. The present results should contribute to improved industrial
production of EG by P. pinophilum KMJ601. 相似文献
44.
Thottethodi Subrahmanya Keshava Prasad Shivakumar Keerthikumar Raghothama Chaerkady Kumaran Kandasamy Santosh Renuse Arivusudar Marimuthu Abhilash Karavattu Venugopal Joji Kurian Thomas Harrys K. C. Jacob Renu Goel Harsh Pawar Nandini A. Sahasrabuddhe Venkatarangaiah Krishna Bipin G. Nair Marjan Gucek Robert N. Cole Raju Ravikumar H. C. Harsha Akhilesh Pandey 《Clinical proteomics》2010,6(4):163-173
45.
Liang F Webb P Marimuthu A Zhang S Gardner DG 《The Journal of biological chemistry》2003,278(17):15073-15083
46.
Longevity and factors influencing photosynthesis in tea leaves 总被引:3,自引:0,他引:3
Quadratic relationship between the age of a tea leaf and the net photosynthetic rate (PN) has been found. A progressive increase in PN was recorded for four months. Then the PN slowly declined, yet even seven-month-old tea leaves sustained a low PN. In a tea shoot, the PN increased from the first leaf onwards. Besides the physiological maturity and proximity, photon flux density (PFD) played an important role in reducing the PN. The tea leaf PN was influenced by cultivation procedures which in turn disrupted the quantum of PFD transmitted through the canopy. 相似文献
47.
Marimuthu A Feng W Tagami T Nguyen H Jameson JL Fletterick RJ Baxter JD West BL 《Molecular endocrinology (Baltimore, Md.)》2002,16(2):271-286
Residues of the TR that are critical for binding the nuclear receptor corepressor (N-CoR) were identified by testing more than 100 separate mutations of the full-length human TRbeta that scan the surface of its ligand binding domain. The primary inferred interaction surface overlaps the surface described for binding of p160 coactivators, but differs by extending to a novel site underneath which helix 12 rests in the liganded TR, rather than including residues of helix 12. Nonconservative mutations of this surface diminished binding similarly to three isolated N-CoR receptor interaction domains (RIDs), but conservative mutations affected binding variably, consistent with a role for this surface in RID selectivity. The commonality of this surface in binding N-CoR was confirmed for the RXRs and ERs. Deletion of helix 12 increased N-CoR binding by the TR modestly, and by the RXR and ER to a much greater extent, indicating a competition between this helix and the corepressor that regulates the extent of corepressor binding by nuclear receptors. When helix 12 was deleted, N-CoR binding by the ER was stimulated by tamoxifen, and binding by the TR was stimulated by Triac, indicating that helix 12 is not the only feature that regulates corepressor binding. Two additional mutationsensitive surfaces were found alongside helix 1, near the previously described CoR box, and above helix 11, nearby but separate from residues that help link receptor in dimers. Based on effects of selected mutations on T(3) and coactivator binding, and on results of combined mutations of the three sites on corepressor binding, we propose that the second and third surfaces stabilize TR unliganded conformation(s) required for efficient N-CoR binding. In transfection assays mutations of all three surfaces impaired the corepressor-mediated functions of unliganded TR repression or activation. These detailed mapping results suggest approaches for selective modulation of corepressor interaction that include the shape of the molecular binding surface, the competitive occupancy by helix 12, pharmacological stimulation, and specific conformational stabilization. 相似文献
48.
Ravichantar Nithya Siti Aminah Ahmed Chee-Hock Hoe Subash C. B. Gopinath Marimuthu Citartan Suresh V. Chinni Li Pin Lee Timofey S. Rozhdestvensky Thean-Hock Tang 《PloS one》2015,10(3)
Salmonellosis, a communicable disease caused by members of the Salmonella species, transmitted to humans through contaminated food or water. It is of paramount importance, to generate accurate detection methods for discriminating the various Salmonella species that cause severe infection in humans, including S. Typhi and S. Paratyphi A. Here, we formulated a strategy of detection and differentiation of salmonellosis by a multiplex polymerase chain reaction assay using S. Typhi non-protein coding RNA (sRNA) genes. With the designed sequences that specifically detect sRNA genes from S. Typhi and S. Paratyphi A, a detection limit of up to 10 pg was achieved. Moreover, in a stool-seeding experiment with S. Typhi and S. Paratyphi A, we have attained a respective detection limit of 15 and 1.5 CFU/mL. The designed strategy using sRNA genes shown here is comparatively sensitive and specific, suitable for clinical diagnosis and disease surveillance, and sRNAs represent an excellent molecular target for infectious disease. 相似文献
49.
Sridhar Arun Manikandan Dinesh Babu Marimuthu Sathish Kumar Murugesan Manikandan Ramasamy Thirumurugan 《International journal of peptide research and therapeutics》2021,27(2):1429-1440
International Journal of Peptide Research and Therapeutics - The teleost fish skin mucus acts as an important physical and biological barrier that prevents fish from the surrounding environment.... 相似文献
50.