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91.
Guðbjörg Ólafsdóttir Vilhjálmur Svansson Sigurður Ingvarsson Eliane Marti Sigurbjörg Torsteinsdóttir 《Acta veterinaria Scandinavica》2008,50(1):44
One of the prerequisite for developing DNA vaccines for horses are vectors that are efficiently expressed in horse cells. 相似文献
92.
Shamshad A. Khan Priyanka Verma Suchitra Banerjee Arnab Chaterjee Sudeep Tandon Alok Kalra Abdul Khaliq Laiq ur Rahman 《Plant Growth Regulation》2017,81(3):365-376
The flowers of Pyrethrum (Chrysanthemum cinerariaefolium) are known to contain Pyrethrins that are naturally occurring potential insecticide. Hairy roots were induced from leaves of C. cinerariaefolium using Agrobacterium rhizogenes strain A4. The root clones were characterized in to four groups i.e. thick, unbranched (D2 and D5), thin, highly branched (D3), thick, branched (B2) and thick, highly branched (D1, D6). Six established hairy root clones showed the presence of pyrethrin and were selected for elicitation studies. Growth kinetics studies revealed highest growth index in hairy root clone D1 (592.0) followed by D6 and D3 on dry weight basis after 40 days of culture. The maximum pyrethrin content was found in the clone D3 (7.2 mg/g dw) which is comparable to the flowers obtained from the variety “Avadh”. Hairy root clone D2 (5.2 mg/g dw) and D6 (1.3 mg/g dw) contained pyrethrin but in less amount as compared to clone D3. The PCR analysis showed the presence of rol B and rol C genes in all the six hairy root clones while rol A was detected only in D2 clone. The methanolic extract of D3 clone showed antifungal activities against phytopathogenic fungal strains which were found maximum against Curvuleria andropogonis followed by Colletotrichum acutatum and Rhizoctonia solani. Hairy root clones D2, D3 and D6 were elicited with culture filtrate of endophytic fungus (Fusarium oxysporum) and bacteria (Bacillus subtilis). The culture filtrate (4.0?%v/v) of both the fungal and bacterial origin was found to be effective in enhancing the pyrethrin content in all the tested hairy root clones. Clone D3 showed maximum pyrethrin content on elicitation with F. oxysporum (9.7 mg/g dw) and B. subtilis (9.7 mg/g dw) culture filtrate, which is 32?% higher than the non elicited D3 hairy roots (7.2 mg/g dw). F. oxysporum also enhanced the hairy root growth resulting into the higher biomass yield of D3 (50?%) and D2 (76?%) in comparison to control non elicited hairy root clones of D3 and D2, respectively leading to higher pyrethrin yield. 相似文献
93.
Pooja Singh Sana Khan Susheel Kumar Laiq ur Rahman 《Plant Cell, Tissue and Organ Culture》2017,129(1):35-44
Rose-scented geranium is an important aromatic herb, have eminent for oil. The oil of geranium commercially utilized in the perfumery, cosmetic and aromatherapy industries all over the world. It is also helpful to cure many of the diseases, since it possess antibacterial, antifungal, antioxidant, anti-inflammatory and anticancer activities. However rose scented geranium suffer from several biotic and abiotic stresses, which reduced the yield of oil. So there is need to genetically improve the geranium using biotechnological approaches. The present study demonstrates the establishment of direct regeneration and Agrobacterium tumefaciens (LBA4404) mediated transformation protocol in Pelargonium graveolens (cv. CIM-BIO 171). Different media combinations such as benzyl amino purine (BAP), kinetin, naphthalene acetic acid (NAA), and adenine di-sulphate (ADS) were standardised to induce direct regeneration in P. graveolens. The maximum regeneration frequency i.e. 90.56?±?1.2% per explant was achieved from petiolar segments in medium containing 2.5 mg/l BAP, 0.1 mg/l NAA, 1 mg/l ADS. However, with the leaf explants only 45.94?±?2.91% frequency was achieved. In the present study, A. tumefaciens strain LBA4404 was used carrying binary vector pBI121 with the gusA as a reporter gene and neomycin phosphotransferase II (nptII) gene as a plant selectable marker. Parameters like bacterial optical density, infection time, acetosyringone concentration and kanamycin concentration were optimised to achieve maximum transformation frequency (69.5?±?2.3%).The putative transgenic shoots were subsequently rooted on half strength MS medium and successfully transferred to the greenhouse. The transgenic plants were characterised by gus histochemical assay, PCR analysis (nptII-786 bp and gus A- 1707 bp) and Southern hybridization tests using gusA gene probe. The regeneration as well as transformation protocol will no doubt provide the basis to decipher the insights of metabolic pathways in geranium. Also could be useful for genetic improvement, to make it more tolerant/resistant against biotic and abiotic stresses and ultimately fruitful for Indian farmers in agronomic traits like high biomass, oil content, yield and better quality. 相似文献
94.
Nenadić Marija Soković Marina Glamočlija Jasmina Ćirić Ana Perić-Mataruga Vesna Ilijin Larisa Tešević Vele Todosijević Marina Vujisić Ljubodrag Vesović Nikola Ćurčić Srećko 《Applied microbiology and biotechnology》2017,101(3):977-985
Applied Microbiology and Biotechnology - Recently, various insect secretions have been tested as possible antimicrobial agents. In beetles, these secretions are essentially products of various... 相似文献
95.
96.
Żur Iwona Dubas Ewa Krzewska Monika Kopeć Przemysław Nowicka Anna Surówka Ewa Gawrońska Katarzyna Gołębiowska Gabriela Juzoń Katarzyna Malaga Sabina 《Plant Cell, Tissue and Organ Culture》2021,145(2):347-366
Plant Cell, Tissue and Organ Culture (PCTOC) - The effectiveness of microspore embryogenesis (ME) is determined by a complex network of internal and environmental factors. In the present study on... 相似文献
97.
Ragnheiður Fossdal Magnus Böðarsson Páll Ásmundsson Jóhann Ragnarsson Dorien Peters Martijn H. Breuning Ólafur Jensson 《Human genetics》1993,91(6):609-613
We have mainly used 3 highly polymorphic DNA markers, 3HVR (D16S85), 16AC2.5 (D16S291) and SM7 (D16S283), flanking the PKD1 region on chromosome 16p13.3 to establish linkage status in seven Icelandic families with autosomal dominant polycystic kidney disease (ADPKD). In four families, the disease locus is in the PKD1 region, and three families are unlinked to chromosome 16p13.3. In one of the unlinked families, the disease locus is excluded from a part of the long arm of chromosome 2, and we support a theory of more than 2 loci being responsible for ADPKD. Our data confirm the location of the locus YNH24 (D2S44) to chromosome 2q13-q24. 相似文献
98.
Eva Stratilová Danica Mislovičová Marta Kačuráková Eva Machová Nadežda Kolarová Oskar Markovič Hans Jörnvall 《The protein journal》1998,17(2):173-179
Comparisons of known primary structures of polygalacturonases show that extent and localization of potential N-glycosylation sites differ. Some sites are similar in position and adjacent to strictly conserved residues at the potential active site. The presence of N-acetylglucosamine and mannose in the molecules of two homogeneous, major Aspergillus sp. polygalacturonase forms was confirmed by IR spectroscopy. The purification method, based on interaction of the carbohydrate part with concanavalin A immobilized on chlorotriazine bead cellulose, was optimized. Deglycosylation with N-glycosidase F under denaturating and nondenaturating conditions led to molecular mass decreases followed by complete inactivation of the polygalacturonase enzyme activity. These results show the importance of glycosylation in these protein forms, while the comparative patterns establish both variability and some similarities in overall glycosylation architectures. 相似文献
99.
100.