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101.
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Derek J Sloan Andrew Nicolson Alastair RO Miller Nick J Beeching Mike BJ Beadsworth 《Journal of medical case reports》2008,2(1):1-5
Introduction
Electrocardiogram (ECG) abnormalities in patients with blunt chest trauma are diverse and non-specific, but may be indicative of potentially life-threatening conditions.Case presentation
We report a rare case of pneumopericardium with extreme ECG abnormalities after blunt chest trauma in a 22-year-old male. The diagnosis was confirmed using computed tomography (CT) scanning. The case is discussed, together with its differential diagnosis and the aetiology of pneumopericardium and tension pneumopericardium.Conclusion
Pneumopericardium should be distinguished from other pathologies such as myocardial contusion and myocardial infarction because of the possible development of tension pneumopericardium. Early CT scanning is important in the evaluation of blunt chest trauma. 相似文献103.
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Structural proteomics of an archaeon 总被引:20,自引:0,他引:20
Christendat D Yee A Dharamsi A Kluger Y Savchenko A Cort JR Booth V Mackereth CD Saridakis V Ekiel I Kozlov G Maxwell KL Wu N McIntosh LP Gehring K Kennedy MA Davidson AR Pai EF Gerstein M Edwards AM Arrowsmith CH 《Nature structural biology》2000,7(10):903-909
A set of 424 nonmembrane proteins from Methanobacterium thermoautotrophicum were cloned, expressed and purified for structural studies. Of these, approximately 20% were found to be suitable candidates for X-ray crystallographic or NMR spectroscopic analysis without further optimization of conditions, providing an estimate of the number of the most accessible structural targets in the proteome. A retrospective analysis of the experimental behavior of these proteins suggested some simple relations between sequence and solubility, implying that data bases of protein properties will be useful in optimizing high throughput strategies. Of the first 10 structures determined, several provided clues to biochemical functions that were not detectable from sequence analysis, and in many cases these putative functions could be readily confirmed by biochemical methods. This demonstrates that structural proteomics is feasible and can play a central role in functional genomics. 相似文献
105.
Gaetano T. Montelione Cheryl Arrowsmith Mark E. Girvin Michael A. Kennedy John L. Markley Robert Powers James H. Prestegard Thomas Szyperski 《Journal of structural and functional genomics》2009,10(2):101-106
This Perspective, arising from a workshop held in July 2008 in Buffalo NY, provides an overview of the role NMR has played
in the United States Protein Structure Initiative (PSI), and a vision of how NMR will contribute to the forthcoming PSI-Biology
program. NMR has contributed in key ways to structure production by the PSI, and new methods have been developed which are
impacting the broader protein NMR community. 相似文献
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Weontae Lee Matthew Revington Neil A. Farrow Asao Nakamura Naoko Utsunomiya-Tate Yoko Miyake Masatsune Kainosho Cheryl H. Arrowsmith 《Journal of biomolecular NMR》1995,5(4):367-375
Summary [ul-13C/15N]-l-tryptophan was prepared biosynthetically and its dynamic properties and intermolecular interaction with a complex of Escherichia coli trp-repressor and a 20 base-pair operator DNA were studied by heteronuclear isotope-edited NMR experiments. The resonances of the free and bound corepressor (l-Trp) were unambiguously identified from gradient-enhanced 15N–1H HSQC, 13C–1H HSQC, 13C-and 15N-edited 2D NOESY spectra. The exchange off-rate of the corepressor between the bound and free states was determined to be 3.4±0.52 s–1 at 45°C, almost three orders of magnitude faster than the dissociation of the protein-DNA complex. Examination of the experimental NOE buildup curves indicates that it may be desirable to use longer mixing times than would normally be used for a large molecule, in order to detect weak intermolecular NOEs in the presence of exchange. Intermolecular NOEs from bound corepressor to trp-repressor and DNA were analyzed with respect to the mechanism of ligand exchange. This analysis suggests that, in order for the ligand to diffuse out of the complex, there must be significant movement or breathing of the protein and/or DNA.Abbreviations NOESY
nuclear Overhauser enhancement spectroscopy
- HSQC
heteronuclear single-quantum coherence
- PFG
pulsed field gradient
-
l-Trp
l-tryptophan 相似文献
110.