Further characterization and quantitative determination of 5-methoxy-N-methyltryptamine in Phalaris arundinacea

The 100 MHz PMR spectrum of 5-methoxy-N-methyltryptamine (5MMT) isolated from Phalaris arundinacea is elucidated, acid-induced fluorescence characteristics of 5-methoxy- and 5-hydroxytryptamine alkaloids on Avicel and silica gel TLC are described, and the concentration of 5MMT in P. arundinacea is determined by fluorometric TLC scanning.     

Bleaching of photosynthetic pigments inChlorella protothecoides grown in the presence of SANDOZ 9785 (4-chloro-5-dimethylamino-2-phenyl-3 (2H) pyridazinone)

The unicellular green algaChlorella protothecoides was grown in the presence of various concentrations (30–300 μM) of the herbicide SANDOZ 9785 (4-chloro-5-(dimethylamino)-2-phenyl-3 (2H) pyridazinone). When the cells were grown autotrophically in the presence of the herbicide, there was a decrease in chlorophylls and carotenoids estimated on culture volume basis compared to the control. The absorption spectra of carotenes and colourless precursors extracted from photosynthetic particles showed that there was a higher carotenoid precursors/carotene ratio in the treated cells. Similarly the carotenoid precursors/xanthophyll ratio was increased by SANDOZ 9785 treatment. Chlorophylla synthesis appeared to be inhibited more than chlorophyllb synthesis in SANDOZ 9785 treated cells resulting in a decrease in the chlorophylla/b ratio.     

Purification and Partial Characterization of Arginine Decarboxylase from Brassica campestris

Arginine decarboxylase (EC 4.1.1.19) has been purified and characterized from Brassica campestris cv B-9. The enzyme was purified 1120 fold and the recovery was 9%. The mol wt of the enzyme determined by gel filtration was 240 kD with identical subunits of 60 kD. The pH and temperature optima for the enzyme were 8.0 and 30°C respectively. The Km was 0.31mM. Polyamines inhibited the enzyme activity significantly. Immunodiffusion with ADC-specific antibodies showed cross reactivity against purified ADC from Brassica.     

Fast and simple anion-exchange chromatography for large-scale purification of self-complementary oligonucleotides.

A fast and simple anion-exchange chromatography method is described for large-scale purification of synthetic oligonucleotides. Using a single matrix and aqueous solvent system, the two-step chromatographic procedure can handle complex separation problems of self-complementary or G-rich sequences without the use of urea or formaldehyde. The work also demonstrates the complication encountered, possibly due to hairpin formation, in one of the oligomers.     

Microbial electron uptake in microbial electrosynthesis: a mini-review

Journal of Industrial Microbiology & Biotechnology - Microbial electron uptake (EU) is the biological capacity of microbes to accept electrons from electroconductive solid materials. EU has...