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151.
J E Woods P G Arnold J K Masson G B Irons W S Payne 《Plastic and reconstructive surgery》1979,63(2):235-241
Aggressive resection, with individualized reconstruction by several methods, is of value in many patients with radiation necrosis and/or advanced breast cancer of the chest wall. Although this does not always significantly lengthen survival, it can improve the quality of life markedly in many instances. Remarkably large defects can be reconstructed with single-stage procedures. 相似文献
152.
Wilfred N. Arnold 《Current microbiology》1981,6(5):305-308
The kinetics of glutaraldehyde inactivation of a protoplasmic (-fructofuranosidase) and an extracytoplasmic (acid phosphatase) enzyme inSaccharomyces rouxii cells were studied at pH 5.5 and 30°C. The effects of glutaraldehyde concentration (0.5–3%), pH value, and temperature were surveyed by varying the fixation conditions. Cells from 1- to 10-day cultures retained 50–75% of their acid phosphatase activity and 15–24% of their -fructofuranosidase activity after 1-h exposures to 0.5% glutaraldehyde. The surviving -fructofuranosidase activity remained physically cryptic and was revealed only after further membrane perturbation with ethyl acetate. This crypticity barrier disappeared after overnight incubation of the treated cells at 4°C, with or without added glutaraldehyde, during which time the enzyme was resistant to further inactivation. The velocity ratio for raffinose versus sucrose, as substrate, decreased in treated cells, and changes inV
max andK
m
were indicative of frank destruction of some enzyme molecules as well as modification of survivors. A comparable set of changes was also generated by treating cell-free extract with glutaraldehyde. Glutaraldehyde (0.5%) killed all yeast cells at 30°C within 5 min; at 4°C survival rates were quite high—81% after 15 min and 65% after 1 h. The bearing of these examples of enzyme inactivation, permeability barrier abolition, and structural stabilization on the general problems of yeast cytochemistry is discussed. 相似文献
153.
154.
155.
Effects of sodium nitroprusside, nitroglycerin, and sodium azide on levels of cyclic nucleotides and mechanical activity of various tissues. 总被引:22,自引:0,他引:22
Three agents that activate guanylate cyclase, sodium nitroprusside, nitroglycerin and sodium axide, were examined for their effects on cyclic GMP and cyclic AMP accumulation and muscle motility with several tissues. All of these agents, except nitroglycerin with ventricle preparations, increased cyclic GMP levels and did not alter cyclic AMP in incubations of preparations of bovine tracheal smooth muscle, guinea pig tracheal chains, taenia cecum, atria and ventricle, and rat liver and cerebral cortex. Increases in cyclic GMP with these agents occurred with relaxation of smooth muscle preparations and without alteration in the contractility of atrial preparations. These observations support the hypothesis that cyclic GMP accumulation in smooth muscle may be related to relaxation rather than contraction as proposed previously. Relaxation with these agents is not associated with alterations in cyclic AMP levels. Increases in cyclic GMP levels in atrial preparations can also occur without changes in contractile force or rate of contraction. 相似文献
156.
U Tage-Jensen W Arnold O Dietrichson F Hardt U Hopf K H Meyer Zum Büschenfelde J O Nielsen 《BMJ (Clinical research ed.)》1977,1(6055):206-208
With an immunofluorescence technique using rabbit hepatocytes isolated by a non-enzymatic method an autoantibody directed against liver-cell-membrance was identified. Sera from 361 patients with various liver diseases and 274 patients with primary non-hepatic diseases-many associated with non-organ-specific auto-antibodies-were examined. The antibody (LMA) was found in 27 out of 72 patients with hepatitis-B-surgace antigen (HBsAg)-negative chronic active hepatitis and in 17 out of 28 patients with HBsAg-negative non-alcoholic cirrhosis. Only two patients had LMA and HBsAg, and both had chronic active hepatitis. One patient with extrhepatic disease was found to have LMA, and this patient had biochemical evidence of liver disease. Hence there is a close correlation between the presence of LMA and HBsAg-negative chronic inflammatory liver diseases and its detection may help in diagnosis. 相似文献
157.
Robert B. Zurier Jeffrey Doty Arnold Goldenberg 《Prostaglandins & other lipid mediators》1977,13(1):25-31
The cyclic AMP response to prostaglandin E1 (PGE1) was studied in peripheral blood (PB) and synovial fluid (SF) mononuclear cells from patients with rheumatoid arthritis (RA). The PGE1 induced accumulation of cyclic AMP was consistently (7 of 8 patients) less in cell suspensions derived from SF than in suspensions of equivalent numbers of mononuclear cells obtained simultaneously from PB. The high PB/SF cyclic AMP ratio was seen most clearly at the lowest concentration (10−6M) of PGE1 tested. There was no correlation between the patients' therapy and cyclic AMP response to PGE1. The high PB/SF cyclic AMP ratio was not accounted for by the presence of platelets in PB cell suspensions. 相似文献
158.
A high glucose concentration (6%) interfered with production of -lactam antibiotics byCephalosporium acremonium. Production rate of the pathway intermediate, penicillin N, by resting cells harvested from a high glucose fermentation, peaked and declined early in the fermentation. When cells were grown in the standard medium (2.7% glucose + 3.6% sucrose), penicillin N productivity was prolonged, showing two peaks, the first during trophophase and the second afterwards. The decline in productivity was not prevented by addition of the amino acid precursors of -lactam antibiotics. The addition of glucose to resting cells drastically decreased formation of the end product, cephalosporin C, but had only a moderate effect on penicillin N production. Glucose markedly repressed the ring-expansion enzyme (deacetoxy-cephalosporin C synthetase) but had a lesser effect on the tripeptide cyclization enzyme (isopenicillin N synthetase). We conclude that the major effect of a low (2%) or a high (6%) concentration of a rapidly used carbon source (e.g., glucose, glycerol, maltose) onC. acremonium fermentations is repression of the metabolically unstable ring-expansion enzyme and hence of formation of cephalosporins. On the other hand, the lesser degree of repression of the cyclization enzyme and itsin vivo stability allow penicillin N to accumulate normally or even at increased rates except at high carbon source concentrations. 相似文献
159.
Saccharification of Complex Cellulosic Substrates by the Cellulase System from Clostridium thermocellum 总被引:37,自引:15,他引:22 下载免费PDF全文
Eric A. Johnson Mitsuji Sakajoh Geoffrey Halliwell Ashwin Madia Arnold L. Demain 《Applied microbiology》1982,43(5):1125-1132
True cellulase activity has been demonstrated in cell-free preparations from the thermophilic anaerobe Clostridium thermocellum. Such activity depends upon the presence of Ca2+ and a thiol-reducing agent of which dithiothreitol is the most promising. Under these conditions, native (cotton) and derived forms of cellulose (Avicel and filter paper) were all extensively solubilized at rates comparable with cellulase from Trichoderma reesei. Maximum activity of the Clostridium cellulase was displayed at 70°C and at pH 5.7 and 6.1 on Avicel and carboxymethylcellulose, respectively. In the absence of substrate at temperatures up to 70°C, carboxymethylcellulase was much more unstable than the Avicel-hydrolyzing activity. 相似文献
160.
John H. Collins Bliss Forbush Lois K. Lane Eleanor Ling Arnold Schwartz Anita Zot 《生物化学与生物物理学报:生物膜》1982,686(1):7-12
Highly purified lamb kidney (Na++K+)-ATPase was photoaffinity labeled with the tritiated 2-nitro-5-azidobenzoyl derivative of ouabain (NAB-ouabain). The labeled (Na++K+)-ATPase was mixed with unlabeled carrier enzyme. Two proteolipid (γ1 and γ2) fractions were then isolated by chromatography on columns of Sepharose CL-6B and Sephadex LH-60. The two fractions were interchangeable when rechromatographed on the LH-60 column, suggesting that γ1 is an aggregated form of γ2. The total yield was 0.8–1.5 mol of γ component per mol of catalytic subunit recovered. This indicates that the γ component is present in stoichiometric amounts in the (Na++K+)-ATPase. The proteolipids that were labeled with NAB-ouabain copurified with the unlabeled proteolipids. 相似文献