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991.
Guillaume J. M. Laugier Gilles Le Moguédec Wang Su Ashraf Tayeh Laurent Soldati Bruno Serrate Arnaud Estoup Benoît Facon 《Biological invasions》2016,18(10):2871-2881
Understanding biological invasion is currently one of the main scientific challenges for ecologists. The introduction process is crucial for the success of an invasion, especially when it involves a demographic bottleneck. A small introduced population is expected to face a higher risk of extinction before the first stage of invasion is complete if inbreeding depression, caused by the expression of deleterious alleles, is important. Changes in mating regimes or in population size can induce the evolution of deleterious allele frequencies, either by selection or by drift, possibly resulting in the purging or the fixation of such alleles within the population. The harlequin ladybird Harmonia axyridis became invasive on several continents following a scenario including at least one event of demographic bottleneck. Although native populations suffered from severe inbreeding depression, it was greatly reduced in invasive ones suggesting that deleterious alleles were purged during the invasion process. In this study, we performed an experiment designed to manipulate the effective population size of H. axyridis across successive generations to mimic contrasting introduction events. We used the measurement of two fitness-related phenotypic traits in order to test (1) if inbreeding depression can evolve at the time-scale of an invasion; and (2) if the changes in inbreeding depression following a bottleneck in laboratory conditions are compatible with the purging of deleterious alleles observed in this species. We found that two generations of very low population size are enough to induce a substantial change in inbreeding depression. Although the genetic changes mostly consisted in fixation of deleterious alleles, purging did also occur, sometimes simultaneously with fixation. 相似文献
992.
Camille Detree Apolline Chabenat Fran?ois H. Lallier Nori Satoh Eiichi Shoguchi Arnaud Tanguy Jean Mary 《PloS one》2016,11(2)
The aim of this study was first to identify lysozymes paralogs in the deep sea mussel Bathymodiolus azoricus then to measure their relative expression or activity in different tissue or conditions. B. azoricus is a bivalve that lives close to hydrothermal chimney in the Mid-Atlantic Ridge (MAR). They harbour in specialized gill cells two types of endosymbiont (gram—bacteria): sulphide oxidizing bacteria (SOX) and methanotrophic bacteria (MOX). This association is thought to be ruled by specific mechanism or actors of regulation to deal with the presence of symbiont but these mechanisms are still poorly understood. Here, we focused on the implication of lysozyme, a bactericidal enzyme, in this endosymbiosis. The relative expression of Ba-lysozymes paralogs and the global anti-microbial activity, were measured in natural population (Lucky Strike -1700m, Mid-Atlantic Ridge), and in in situ experimental conditions. B. azoricus individuals were moved away from the hydrothermal fluid to induce a loss of symbiont. Then after 6 days some mussels were brought back to the mussel bed to induce a re-acquisition of symbiotic bacteria. Results show the presence of 6 paralogs in B. azoricus. In absence of symbionts, 3 paralogs are up-regulated while others are not differentially expressed. Moreover the global activity of lysozyme is increasing with the loss of symbiont. All together these results suggest that lysozyme may play a crucial role in symbiont regulation. 相似文献
993.
Arnaud Billet Yanlin Jia Timothy J. Jensen Yue-Xian Hou Xiu-Bao Chang John R. Riordan 《Channels (Austin, Tex.)》2016,10(3):247-251
The CFTR chloride channel is tightly regulated by phosphorylation at multiple serine residues. Recently it has been proposed that its activity is also regulated by tyrosine kinases, however the tyrosine phosphorylation sites remain to be identified. In this study we examined 2 candidate tyrosine residues near the boundary between the first nucleotide binding domain and the R domain, a region which is important for channel function but devoid of PKA consensus sequences. Mutating tyrosines at positions 625 and 627 dramatically reduced responses to Src or Pyk2 without altering the activation by PKA, suggesting they may contribute to CFTR regulation. 相似文献
994.
995.
996.
Gaëtan Maertens Oscar M. Saavedra Vito Vece Miguel A. Vilchis Reyes Sofiane Hocine Esat Öney Bertrand Goument Olivier Mirguet Arnaud Le Tiran Philippe Gloanec Stephen Hanessian 《Bioorganic & medicinal chemistry letters》2018,28(15):2627-2630
We have developed versatile methods toward the synthesis of a variety of piperidine/piperazine bridged isosteres of pridopidine. The compounds were assessed against the D2 receptor in agonist and antagonist modes and against the D4 receptor in agonist mode. hERG Binding and the ADME profiles were studied. 相似文献
997.
Arpita Dalal Ross N. Cuthbert Jaimie T.A. Dick Arnaud Sentis Ciaran Laverty Daniel Barrios-O'Neill Natali O. Perea Amanda Callaghan Susmita Gupta 《Ecological Entomology》2020,45(3):423-433
1. Interactions between multiple predators can modify prey risk and profoundly alter ecological community dynamics. Further, ontogenic prey size changes are known to mediate prey risk through refuge effects. Understandings of these biotic factors is important for robust quantifications of natural enemy effects on target species, yet their combined influence lacks investigation. 2. Functional responses were used to quantify the predatory impacts of Notonecta glauca (water boatman; Ng) and Gammarus pulex (river shrimp; Gp) towards four different larval instars of Culex pipiens in container-style habitats. Using conspecific pairs of predators, multiple predator effects (MPEs) of both predator species were examined across larval prey sizes, and prey preference tests were applied to examine prey selectivity across predator–prey body size ratios. 3. Both predators were able to feed on C. pipiens across their larval ontogeny; however, Ng consumed significantly more larvae than Gp. Functional responses of Ng were typically Type IIs, whereas Gp trended towards sigmoidal Type IIIs. Predation by pairs of Ng and Gp showed independent MPEs towards first-, third-, and fourth-instar stages (except predation by Gp at higher densities of fourth-instar) stages, whereas, for second-instar stages, Ng showed synergistic MPEs and Gp showed antagonistic MPEs. Both predators preferred late instar mosquitoes (Ng: fourth instar; Gp: third instar). These preferences reflected predator:prey weight–length ratios, showing that relative sizes of predators and prey are important factors in prey selectivity. 4. The results obtained in the present study demonstrate that MPEs, combined with intraspecific prey preferences, may modulate trophic interactions within ecosystems. Therefore, such effects should be increasingly considered to further the understanding of agent efficacies. 相似文献
998.
Else J Fjerdingstad Nicolas Schtickzelle Pauline Manhes Arnaud Gutierrez Jean Clobert 《BMC evolutionary biology》2007,7(1):133
Background
Considerable attention has focused on how selection on dispersal and other core life-history strategies (reproductive effort, survival ability, colonization capacity) may lead to so-called dispersal syndromes. Studies on genetic variation in these syndromes within species could importantly increase our understanding of their evolution, by revealing whether traits co-vary across genetic lineages in the manner predicted by theoretical models, and by stimulating further hypotheses for experimental testing. Yet such studies remain scarce. Here we studied the ciliated protist Tetrahymena thermophila, a particularly interesting organism due to cells being able to transform into morphs differing dramatically in swim-speed. We investigated dispersal, morphological responses, reproductive performance, and survival in ten different clonal strains. Then, we examined whether life history traits co-varied in the manner classically predicted for ruderal species, examined the investment of different strains into short- and putative long-distance dispersal, while considering also the likely impact of semi-sociality (cell aggregation, secretion of 'growth factors') on dispersal strategies. 相似文献999.
1000.
We have noticed important metabolic modifications during the first 15 hours of the sporulation process of yeast in contact with acetic acid. Carbohydrate reserves, dry weight and proteins increase. On the other hand, the activity of fumarase and isocitritase in cell homogenates are hardly altered. The results prove true the hypothesis according to which the metabolism of acetic acid is altered during sporulation.
Zusammenfassung Beträchtliche metabolische Veränderungen wurden bei Hefe, während der fünfzehn ersten Stunden der Sporenbildung unter Zugabe von Essigsäure, beobachtet. Der Kohlenhydratenvorrat, die Trockensubstanz und der Proteingehalt nehmen zu. Die Aktivität der Fumarase und Isocitritase wird kaum verändert. Dadurch wird die Annahme bestätigt, daß eine Abweichung des Metabolismus der Essigsäure während der Sporenbildung stattfindet.相似文献