Prostaglandin I2 stimulation of granulosa cell cyclic AMP production

The ability of prostaglandin I₂ (PGI₂) to stimulate cyclic AMP production by granulosa cells, isolated from intact immature rats, has been demonstrated in vitro. The minimal effective dose was 15 ng/ml, which was comparable to the minimal effective dose for PGE₂. However, a concentration of 15 μg/ml PGI₂ was required to stimulate cyclic AMP production maximally, compared to a concentration of 1 μg/ml PGE₂, which produced the maximum response. It therefore appears that PGI₂ is not more effective than PGE₂ in stimulating cyclic AMP production in granulosa cells, and is possibly less effective. Submaximal concentrations of PGI₂ appeared to be able to modify the stimulation of cyclic AMP production by follicle- stimulating hormone (FSH), but whether or not PGI₂ plays any role in follicular function remains to be established.     

Metabolic role of free mycolic acids in Mycobacterium tuberculosis.

Small amounts of free mycolic acids and trehalose dimycolate that are rapidly formed by Mycobacterium tuberculosis H37Ra are probably derived from mycolyl acetyl trehalose and transferred to the cell wall. However, the transfer of mycolic acids from mycolyl acetyl trehalose to the cell wall still appears to be the more prominent route.     

Hepatic microsomal epoxide hydrase. Chemical evidence for a single polypeptide chain.

Highly purified hepatic microsomal epoxide hydrase, which had been purified in the presence of proteolytic enzyme inhibitors, was subjected to carboxypeptidase Y digestion, automated Edman degradation, and carbohydrate analysis. Carboxypeptidase Y digestion resulted in the near stoichiometric release of leucine, the COOH-terminal amino acid. Automated Edman degradation permitted the identification of the first 20 amino acid residues of epoxide hydrase. Methionine was identified as the NH2-terminal residue. The NH2-terminal region of epoxide hydrase is similar in hydrophobicity to the NH2-terminal precursor segments of several secretory proteins and the NH2-terminal regions of several microsomal cytochromes P-450. Carbohydrate analyses of the enzyme revealed the presence of 0.5 to 1.0 mol of mannose/50,000 g of protein. These results provide evidence for the presence of a single polypeptide chain in our purified enzyme preparations and suggest that there may be only one enzymic form of epoxide hydrase in microsomes from phenobarbital-treated rats.     

Distribution of Cytokinin-active Ribonucleosides in Wheat Germ tRNA Species

The distribution of cytokinin activity in wheat (Triticum aestivum) germ tRNA fractionated by BD-cellulose and RPC-5 chromatography has been examined. As in other organisms, the cytokinin moieties in wheat germ tRNA appear to be restricted to tRNA species that would be expected to respond to codons beginning with U. Only a few of the wheat germ tRNA species in this coding group actually contain cytokinin modifications. Cytokinin activity was associated with isoaccepting tRNA^Ser species and with a minor tRNA^Leu species from wheat germ. All other wheat germ tRNA species corresponding to codons beginning with U were devoid of cytokinin activity in the tobacco callus bioassay.     

Down and Turner syndromes in a female infant with 47,X,del(X)(p11),+21

Summary This report describes a female infant with a 47,X,del(X)(p11),+21 karyotype who has clinical features of both Down and Turner syndromes. The majority of her clinical features are suggestive of Down syndrome.     

Differential cytokinin structure-activity relationships in phaseolus

The activities of eight cytokinins in promoting callus growth were tested in two Phaseolus genotypes, P. vulgaris L. var. Great Northern, and P. lunatus L. var. Kingston. The structural feature which contributes to the major genotypic difference in cytokinin structure-activity relationships is the presence or absence of a double bond at the 2,3-position of the isoprenoid N⁶ side chain. In Kingston, trans-zeatin was 3-fold more active than dihydrozeatin and 30-fold more active than cis-zeatin. The activities of N⁶-(Δ²-isopentenyl)adenine and N⁶-isopentyladenine were nearly the same. In Great Northern, however, dihydrozeatin was at least 30-fold more active than both trans-zeatin and cis-zeatin, and N⁶-isopentyladenine was 100-fold more active than N⁶-(Δ²-isopentenyl)adenine. The results suggest the possibility of employing cytokinin structure-activity relationships in distinguishing genotypic differences in cytokinin function and metabolism.     

Increases in nuclear volume and cell size in meristematic cells arrested by 5-aminouracil

Summary Lateral roots ofVicia faba were treated with a solution of 5-aminouracil (3.93 × 10^–3 M). They were either treated for 6 hours and allowed to recover for up to 10 hours, or were treated continuously for up to 24 hours. Mitotic index decreased as the duration of treatment increased,e.g., it was < 0.5 after 6 hours treatment and 4 hours recovery and 0.23 after 12 hours continuous treatment. During this period of low mitotic activity nuclei and cells increased in size: mean nuclear volume, for example, was 1505±651 m³ 8 hours after the end of a 6 hours treatment. In roots treated continuously, nuclear volume increased from 559±204 m³ at 0 hour to 1272±636 m³ at 12 hours. In the first 3 hours it was the larger nuclei that grew,i.e., nuclei that would have proceeded into mitosis if they had not been blocked by 5-AU. But between 3 and 12 hours of continuous exposure to 5-AU all nuclei increased in volume. Cells, on the other hand, showed no response during the first 6 hours of treatment; their areas did not increase till 6–12 hours had elapsed. It appears that in cells blocked by 5-AU growth continues for about 12 hours. Initially, nuclei grow disproportionately large, suggesting that synthesis of nuclear components is favoured at the expense of cytoplasmic constituents, at least during the first 6 hours of treatment; there is an internal imbalance between nuclear and cell growth and a temporary change in the nuclear cytoplasmic ratio. When cells recover from the 5-AU block and enter mitosis their prophase nuclei are also much larger than those of untreated cells. The response to 5-AU is discussed in terms of internal restrictions on cell growth, due to the presence of cell walls, and the heterogeneity in nuclear volumes.     

Inhibition of light-induced pH increase and O2 evolution of marine microalgae by water-soluble components of crude and refined oils.

Light-induced alkalinization of the extracellular medium was found to be a common feature of the primary photosynthetic process of several marine microalgae. The light-induce PH increase of suspensions of whole cells was immediately and severely inhibited by a single dose of water-soluble components from crude and fuel oils. Differential effects on the rates of microalgal photosynthetic O2 evolution and cell suspension pH increase suggest different toxicity mechanisms of the water-soluble components of no. 2 fuel oil as compared with Southern Louisiana and Jay Crude oils. These short-term studies on the nature of sublethal petroleum toxicity to microalgae indicate that the primary effect may be through direct action on the energy-yielding electron transport systems.