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91.
Mismatch of morphological and molecular identifications in native and invasive subspecies of Codium fragile (Bryopsidophyceae,Chlorophyta) 下载免费PDF全文
Several subspecies are defined within Codium fragile, including the invasive C. fragile ssp. fragile, first reported in New Zealand in 1973. An endemic subspecies, C. fragile ssp. novae‐zelandiae, is also found throughout New Zealand. The two subspecies exhibit morphological and molecular variation, although these have never been evaluated together. We compared variation between subspecies at locations in Auckland, identifying subspecies using rps3‐rpl16 DNA sequence data, and assessing gross morphological differences, anatomical utricle characters and morphometrics. The taxonomic utility of the morphometric data sets was assessed by linear discriminant analysis. Utricle characters and measurements varied within individual thalli and between different preservation methods. The phenotypes of both subspecies were highly variable and influenced by environment. Accurate subspecies delimitation using morphological data was not possible; the discriminant analyses performed no better than chance for all combinations of the morphological data. Specimens from New Zealand, Canada, Australia and Ireland were sequenced using both the rps3‐rpl16 and tufA plastid markers. The tufA elongation factor was shown to be a good candidate for differentiating subspecies of C. fragile. This marker is twice the length of the rps3‐rpl16 spacer, shows greater variation between ssp. fragile and novae‐zelandiae, and is less prone to sequencing error. A simple restriction enzyme digest of the tufA amplicon can distinguish ssp. fragile and ssp. novae‐zelandiae. Our study expands the known range of the ssp. fragile in New Zealand, including the first record of this subspecies from the west coast of Auckland, and points to a need to re‐evaluate morphological and molecular criteria for subspecies currently defined within C. fragile. 相似文献
92.
Spatial and temporal modelling of parasite transmission and risk assessment require relevant spatial information at appropriate spatial and temporal scales. There is now a large literature that demonstrates the utility of satellite remote sensing and spatial modelling within geographical information systems (GIS) and firmly establishes these technologies as the key tools for spatial epidemiology. This review outlines the strength of satellite remotely sensed data for spatial mapping of landscape characteristics in relation to disease reservoirs, host distributions and human disease. It is suggested that current satellite technology can fulfill the spatial mapping needs of disease transmission and risk modelling, but that temporal resolution, which is a function of the satellite data acquisition characteristics, may be a limitating factor for applications requiring information about landscape or ecosystem dynamics. The potential of the Modis sensor for spatial epidemiology is illustrated with reference to mapping spatial and temporal vegetation dynamics and small mammal parasite hosts on the Tibetan plateau. Future research directions and priorities for landscape epidemiology are considered. 相似文献
93.
Rhodobacter sphaeroides: complexity in chemotactic signalling 总被引:1,自引:0,他引:1
Most bacteria have much more complex chemosensory systems than those of the extensively studied Escherichia coli. Rhodobacter sphaeroides, for example, has multiple homologues of the E. coli chemosensory proteins. The roles of these homologues have been extensively investigated using a combination of deletion, subcellular localization and phosphorylation assays. These studies have shown that the homologues have specific roles in the sensory pathway, and they differ in their cellular localization and interactions with other components of the pathway. The presence of multiple chemosensory pathways might enable bacteria to tune their tactic responses to different environmental conditions. 相似文献
94.
Rachel Stubbington Adam M. Greenwood Paul J. Wood Patrick D. Armitage John Gunn Anne L. Robertson 《Hydrobiologia》2009,630(1):299-312
The headwaters of karst rivers experience considerable hydrological variability, including spates and streambed drying. Extreme
summer flooding on the River Lathkill (Derbyshire, UK) provided the opportunity to examine the invertebrate community response
to unseasonal spate flows, flow recession and, at temporary sites, streambed drying. Invertebrates were sampled at sites with
differing flow permanence regimes during and after the spates. Following streambed drying at temporary sites, dewatered surface
sediments were investigated as a refugium for aquatic invertebrates. Experimental rehydration of these dewatered sediments
was conducted to promote development of desiccation-tolerant life stages. At perennial sites, spate flows reduced invertebrate
abundance and diversity, whilst at temporary sites, flow reactivation facilitated rapid colonisation of the surface channel
by a limited number of invertebrate taxa. Following streambed drying, 38 taxa were recorded from the dewatered and rehydrated
sediments, with Oligochaeta being the most abundant taxon and Chironomidae (Diptera) the most diverse. Experimental rehydration
of dewatered sediments revealed the presence of additional taxa, including Stenophylax sp. (Trichoptera: Limnephilidae) and Nemoura sp. (Plecoptera: Nemouridae). The influence of flow permanence on invertebrate community composition was apparent despite
the aseasonal high-magnitude flood events. Flow permanence was also critical in determining the community response to the
spate flows. Following streambed drying at temporary sites, the surficial sediments overlying the karstic bedrock functioned
as an effective refugium for several taxa. The development of aquatic insects following experimental rehydration indicated
that these taxa survived in dewatered sediments as desiccation-resistant eggs.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Handling editor: Sonja Stendera 相似文献
95.
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97.
Thomas A. Frankovich Anna R. Armitage Ania H. Wachnicka Evelyn E. Gaiser James W. Fourqurean 《Journal of phycology》2009,45(5):1010-1020
A field experiment was employed in Florida Bay investigating the response of seagrass epiphyte communities to nitrogen (N) and phosphorus (P) additions. While most of the variability in epiphyte community structure was related to uncontrolled temporal and spatial environmental heterogeneity, P additions increased the relative abundance of the red algae–cyanobacterial complex and green algae, with a concomitant decrease in diatoms. When N was added along with P, the observed changes to the diatoms and the red algae–cyanobacterial complex were in the same direction as P‐only treatments, but the responses were decreased in magnitude. Within the diatom community, species relative abundances, species richness, and diversity responded weakly to nutrient addition. P additions produced changes in diatom community structure that were limited to summer and were stronger in eastern Florida Bay than in the western bay. These changes were consistent with well‐established temporal and spatial patterns of P limitation. Despite the significant change in community structure resulting from P addition, diatom communities from the same site and time, regardless of nutrient treatment, remained more similar to one another than to the diatom communities subject to identical nutrient treatments from different sites and times. Overall, epiphyte communities exhibited responses to P addition that were most evident at the division level. 相似文献
98.
Mark AJ Roberts Elias August Abdullah Hamadeh Philip K Maini Patrick E McSharry Judith P Armitage Antonis Papachristodoulou 《BMC systems biology》2009,3(1):105-14
Background
Developing methods for understanding the connectivity of signalling pathways is a major challenge in biological research. For this purpose, mathematical models are routinely developed based on experimental observations, which also allow the prediction of the system behaviour under different experimental conditions. Often, however, the same experimental data can be represented by several competing network models. 相似文献99.
Cullen-McEwen LA Armitage JA Nyengaard JR Moritz KM Bertram JF 《American journal of physiology. Renal physiology》2011,300(6):F1448-F1453
Low glomerular (nephron) endowment has been associated with an increased risk of cardiovascular and renal disease in adulthood. Nephron endowment in humans is determined by 36 wk of gestation, while in rats and mice nephrogenesis ends several days after birth. Specific genes and environmental perturbations have been shown to regulate nephron endowment. Until now, design-based method for estimating nephron number in developing kidneys was unavailable. This was due in part to the difficulty associated with unambiguously identifying developing glomeruli in histological sections. Here, we describe a method that uses lectin histochemistry to identify developing glomeruli and the physical disector/fractionator principle to provide unbiased estimates of total glomerular number (N(glom)). We have characterized N(glom) throughout development in kidneys from 76 rats and model this development with a 5-parameter logistic equation to predict N(glom) from embryonic day 17.25 to adulthood (r(2) = 0.98). This approach represents the first design-based method with which to estimate N(glom) in the developing kidney. 相似文献
100.
El Ghazi I Martin BL Armitage IM 《Experimental biology and medicine (Maywood, N.J.)》2006,231(9):1500-1506
Metallothionein (MT)-3, originally called growth inhibitory factor (GIF), was initially identified through its ability to inhibit the growth of neuronal cells in the presence of brain extract. MT-3 is the brain specific isoform of the MT family whose specific biological activity associates it with neurological disorders. Indeed, studies report that MT-3 is decreased by ~30% in brains of patients with Alzheimer disease (AD). Furthermore, many lines of evidence suggest that MT-3 engages in specific protein interactions. To address this, we conducted immunoaffinity chromatography experiments using an immobilized anti-mouse MT-3 antibody. We identified five associated proteins from the pool of sixteen recovered using mass spectrometry and tandem mass spectrometry after in-gel trypsin digestion of bands from the affinity chromatography. The proteins identified were: heat shock protein 84 (HSP84), heat shock protein 70 (HSP70), dihydropyrimidinase-like protein-2 (DRP-2), creatine kinase (CK) and beta-actin. Coimmunoprecipitation experiments, also conducted on whole mouse brain extract using the anti-mouse MT-3 antibody along with commercially available antibodies against HSP84 and CK, confirmed that these three proteins were in a single protein complex. Immunohistochemical experiments were then conducted on the perfused mouse brain that confirmed the in situ colocalization of CK and MT-3 in the hippocampus region. These data provide new insights into the involvement of MT-3 in a multiprotein complex, which will be used to understand the biological activity of MT-3 and its role in neurological disease. 相似文献