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101.
D. M. Gadbois W. L. Salo D. K. Ann S. W. Downing D. M. Carlson 《Preparative biochemistry & biotechnology》2013,43(1):67-76
The isolation of translatable poly(A)±mRNA from the slime glands of the Pacific hagfish, Eptatretus stouti, is not possible by the commonly used procedures because of the viscous slime that is formed when the contents of the glands are hydrated. This paper reports on a procedure developed to overcome this problem. Briefly, the tissue was powdered in liquid nitrogen, mixed with sodium lauroylsarcosine and proteinase K and lyophilized. The lyophilized powder was then mixed with 0.3 mm diameter glass beads, thoroughly ground and wetted with buffer and digested at 37°C. The RNA from the digest was recovered by ultracentrifugation through a CsCl cushion. Further purification of the RNA was accomplished by the usual methods with slight modifications. 相似文献
102.
Jari Hovinen Alex Azhayev Harri Salo Juhani Vilpo 《Nucleosides, nucleotides & nucleic acids》2013,32(6-7):1263-1264
Abstract The title compounds (1a,b) were synthesized in three steps from 2-deoxy-D-ribose, and used in the preparation of oligonucleotide conjugates, branched oligonucleotides as well as homo-N-nucleosides. 相似文献
103.
V Perhoniemi H Salo S Sundberg 《European journal of applied physiology and occupational physiology》1986,55(6):630-633
In 20 patients we studied the changes in calf arterial blood flow (AF) following spinal and epidural blocks, using venous occlusion strain gauge plethysmography (SGP). AF was calculated both in the conventional way by drawing a tangent to the initial upslope of the curve, and by a new method which measures the time to the point when 50% of venous capacity is reached (tVC50). The statistical differences within and between the spinal and epidural groups for AF and tVC50 were determined. In measuring the post-block changes in AF as compared with the control values, a statistically significant correlation (rs = -0.85, p less than 0.01) was observed between the two methods. We conclude that the new variable, tVC50, seems to be potentially useful in calculating changes in arterial blood flow at rest, and could be used, for example, in connection with surgical and pharmacological interventions, especially when the initial upslope of the SGP curve is equivocal. 相似文献
104.
Olivari S Cali T Salo KE Paganetti P Ruddock LW Molinari M 《Biochemical and biophysical research communications》2006,349(4):1278-1284
Proteins expressed in the endoplasmic reticulum (ER) are covalently modified by co-translational addition of pre-assembled core glycans (glucose(3)-mannose(9)-N-acetylglucosamine(2)) to asparagines in Asn-X-Ser/Thr motifs. N-Glycan processing is essential for protein quality control in the ER. Cleavages and re-additions of the innermost glucose residue prolong folding attempts in the calnexin cycle. Progressive loss of mannoses is a symptom of long retention in the ER and elicits preparation of terminally misfolded polypeptides for dislocation into the cytosol and proteasome-mediated degradation. The ER stress-induced protein EDEM1 regulates disposal of folding-defective glycoproteins and has been described as a mannose-binding lectin. Here we show that elevation of the intralumenal concentration of EDEM1 accelerates ER-associated degradation (ERAD) by accelerating de-mannosylation of terminally misfolded glycoproteins and by inhibiting formation of covalent aggregates upon release of terminally misfolded ERAD candidates from calnexin. Acceleration of Man(9) or Man(5)N-glycans dismantling upon overexpression was fully blocked by substitution in EDEM1 of one catalytic residue conserved amongst alpha1,2-mannosidases, thus suggesting that EDEM1 is an active mannosidase. This mutation did not affect the chaperone function of EDEM1. 相似文献
105.
Parkkari T Salo OM Huttunen KM Savinainen JR Laitinen JT Poso A Nevalainen T Järvinen T 《Bioorganic & medicinal chemistry》2006,14(8):2850-2858
Results from a factor analysis and activity studies of commercially available endocannabinoid-type compounds set the starting point for the current study where dimethylheptyl (DMH) analogues of two endocannabinoids, 2-arachidonoyl glycerol (2-AG) and 2-arachidonyl glyceryl ether (2-AGE), were synthesized and their ability to activate the CB1 receptors was determined by the [35S]GTPgammaS binding assay using rat cerebellar membranes. The main goal of the study was to examine how the DMH end tail affects the activity properties of 2-AG (1) and its stable ether (2) and urea analogues (5). The importance of the chain length was also explored by synthesizing 2-AG and 2-AGE derivatives (3 and 4) possessing the chain length C21 instead of C22. Replacement of the pentyl end chain with the DMH resulted in distinct potency decrease as compared to the reference compounds. The modification did not have such a strong impact on the efficacy values. In fact, the efficacy of the derivatives of 2-AGE (2 and 4) was comparable or even improved. Introducing a more stable and hydrophilic urea bond led to a dramatic decrease in biological activity. 相似文献
106.
107.
Steffi Heidenreich Pamela Weber Heike Stephanowitz Konstantin M. Petricek Till Schütte Moritz Oster Antti M. Salo Miriam Knauer Isabel Goehring Na Yang Nicole Witte Anne Schumann Manuela Sommerfeld Matthias Muenzner Johanna Myllyharju Eberhard Krause Michael Schupp 《The Journal of biological chemistry》2020,295(50):17158
108.
Kelly Devereaux Claudia Dall’Armi Abel Alcazar-Roman Yuta Ogasawara Xiang Zhou Fan Wang Akitsugu Yamamoto Pietro De Camilli Gilbert Di Paolo 《PloS one》2013,8(10)
Synthesis of phosphatidylinositol-3-phosphate (PI3P) by Vps34, a class III phosphatidylinositol 3-kinase (PI3K), is critical for the initial steps of autophagosome (AP) biogenesis. Although Vps34 is the sole source of PI3P in budding yeast, mammalian cells can produce PI3P through alternate pathways, including direct synthesis by the class II PI3Ks; however, the physiological relevance of these alternate pathways in the context of autophagy is unknown. Here we generated Vps34 knockout mouse embryonic fibroblasts (MEFs) and using a higher affinity 4x-FYVE finger PI3P-binding probe found a Vps34-independent pool of PI3P accounting for ~35% of the total amount of this lipid species by biochemical analysis. Importantly, WIPI-1, an autophagy-relevant PI3P probe, still formed some puncta upon starvation-induced autophagy in Vps34 knockout MEFs. Additional characterization of autophagy by electron microscopy as well as protein degradation assays showed that while Vps34 is important for starvation-induced autophagy there is a significant component of functional autophagy occurring in the absence of Vps34. Given these findings, class II PI3Ks (α and β isoforms) were examined as potential positive regulators of autophagy. Depletion of class II PI3Ks reduced recruitment of WIPI-1 and LC3 to AP nucleation sites and caused an accumulation of the autophagy substrate, p62, which was exacerbated upon the concomitant ablation of Vps34. Our studies indicate that while Vps34 is the main PI3P source during autophagy, class II PI3Ks also significantly contribute to PI3P generation and regulate AP biogenesis. 相似文献
109.
Peter Mašán Maria Alejandra Perotti Marta Inés Saloña-Bordas Henk Ronald Braig 《Experimental & applied acarology》2013,61(4):415-429
Proctolaelaps euserratus Karg, 1994 (Acari, Mesostigmata, Melicharidae), exclusivelly known from the Galápagos Islands till now, is newly reported from decaying matter of animal and human decomposition in various countries of Europe (Slovakia, Spain, United Kingdom). In consequence of high levels of necrophilia, the species is considered to be ecologically unusual among the other melicharids, which are primary associated with other than necrophilic habitats, such as galleries of subcorticolous beetles, bumble bee nests, flowers, etc. Proctolaelaps euserratus is reviewed, morphologically re-described (with first diagnostic characters for males), and considered as a new potential marker for later stages of decomposition, namely butyric fermentation and dry decomposition as classified in modern concepts of forensic acarology. 相似文献
110.
Pauline F. D. Scheelbeek Marivic V. F. Balagon Florenda M. Orcullo Armi A. Maghanoy Junie Abellana Paul R. Saunderson 《PLoS neglected tropical diseases》2013,7(9)