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81.
82.
Richard T. Ruettinger Bok-Hoi Kim Armand J. Fulco 《Biochimica et Biophysica Acta (BBA)/General Subjects》1984,801(3):372-380
The soluble, cytochrome P-450-dependent fatty acid monooxygenase of Bacillus megaterium ATCC 14581 is induced by phenobarbital and at least twelve other barbiturates (Kim, B.-H. and Fulco, A.J. 91983) Biochem. Biophys. Res. Commun. 116, 843–850). We have since found that the inducer potency of phenobarbital and of six other of these barbiturates was enhanced by adding them to growth medium prior to sterilization by autoclaving. A similar ‘activation’ was effected simply by autoclaving these barbiturates in distilled water at pH 8.0. When the hydrolytic products resulting from such treatment of phenobarbital were identified and screened for inducer activity, the major product, 2-phenulbutyrylurea, was found to be 3–5-times more potent than phenobarbital itself. The racemic mixture, (±-)-2-phenylbutyryluera was somewhat more active as an inducer than was either of the enantiomers (±) or (?) tested singly. Of the other hydrolytic products of phenobarbital, only 2-phenylbutyramide had significant inducer activity (about the same as phenobarbital). Among other ureides tested, tow monosubstituted acetylureas (phenylacetylurea and dodecanoylurea) were inactive as inducers, but six of seven disubstituted acetylureas were better inducers than 2-phenylbutyrylurea. 相似文献
83.
84.
Volume 4 number 1 p.8 Table I: In the column headed HCl (pH)50(3.60) should read 50(1.30) 相似文献
85.
Donald A. Kautter Timothy Lilly Jr. Armand J. LeBlanc Richard K. Lynt 《Applied microbiology》1974,28(4):722
The incidence of Clostridium botulinum in fresh crabmeat of blue crab was six out of 986 samples; in pasteurized crabmeat one sample out of 1,000 contained the organism. 相似文献
86.
Distribution of microaerophilic bacteria through the oxic-anoxic transition zone of lagoon sediments
In marine sediments, where soluble gases diffuse only very slightly, many organisms struggle for molecular oxygen. Microaerophilic bacteria, able to grow at reduced pO2 between 0.2 and 12%, have an advantage. Distribution of aerobes, microaerophiles and anaerobes was compared with the oxygen gradient in seawater and sediment samples collected in a northern Mediterranean lagoon. In the near bottom seawater and in the 0–10 mm upperlayer of sediment, the microaerophilic counts were less than 1% of aerobe densities. In the 10–15 mm zone, these two groups were equivalent in density (1 × 105 cells ml–1). As expected, the microaerophiles took advantage of their low oxygen tension requirements in the subsurface sediments, between the well aerated zone (0–5 mm depth) and the low redox potential zone. Then, beyond a depth of 20 mm, the anaerobes prevailed in this sandy clay. 相似文献
87.
88.
Transcriptome response of the foundation plant Spartina alterniflora to the Deepwater Horizon oil spill 下载免费PDF全文
89.
The gene coding for the green fluorescent protein (GFP) from jellyfish was introduced into conifer tissues by microprojectile bombardment and its transient expression was detected. Two versions of the GFP gene, wild-type GFP and modified GFP with a cryptic intron removed, were directly compared for their expression in black spruce pollen. While the wild-type GFP gene resulted in a low level of expression, the modified GFP gene gave a dramatic increase in amount of expression (>100 times). The expression of GFP was detected in all the tissues tested : pollen, embryonal masses, suspension culture, and somatic embryos. Also, the GFP gene was introduced and expressed in three different conifer species (black spruce, white spruce, and white pine). The successful expression of the GFP gene in various tissues and different species suggests that it will be a useful reporter/marker gene for conifers.Abbreviations GUS
-glucuronidase
- NOS
nopaline synthase
- NPTII
neomycin phosphotransferase
- CaMV
cauliflower mosaic virus
Communicated by S. Gleddie 相似文献
90.
Séguin Armand Laible G?tz Leyva Antonio Dixon Richard A. Lamb Christopher J. 《Plant molecular biology》1997,35(3):281-291