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81.
Arlette Fellous Veena Prasad Renée Ohayon Mary Ann Jordan Richard F. Ludueña 《Journal of Protein Chemistry》1994,13(4):381-391
Microtubule-associated proteins (MAPs) can promote microtubule assemblyin vitro. One of these MAPs (MAP2) consists of a short promoter domain which binds to the microtubule and promotes assembly and a long projection domain which projects out from the microtubule and may interact wth other cytoskeletal elements. We have previously shown that MAP2 and another MAP, tau, differ in their interactions with tubulin in that tau, but not MAP2, promotes extensive aggregation of tubulin into spiral clusters in the presence of vinblastine and that microtubules formed with MAP2 are more resistant than those formed with tau to the antimitotic drug maytansine [Luduena, R. F.,et al. (1984),J. Biol. Chem.
259, 12890–12898; Fellous, A.,et al. (1985),Cancer Res.
45, 5004–5010]. Here we have used chymotryptic digestion to remove the projection domain of MAP2 and examined the interaction of the digested MAP2 (ctMAP2) with tubulin in the presence of vinblastine and maytansine. We have found that ctMAP2 behaves very much like tau, but not like undigested MAP2, in the presence of vinblastine, in that ctMAP2 causes tubulin to polymerize into large clusters of spirals. In contrast, microtubule assembly in the presence of ctMAP2 is much more resistant to maytansine inhibition than is assembly in the presence of tau or undigested MAP2. Our results suggest that the projection domain of MAP2 may play a role in the interaction of tubulin with MAP2 during microtubule assembly.Abbreviations MAPs
microtubule-associated proteins
- ctMAP2
MAP2 digested with-chymotrypsin
- nMAP2
untreated MAP2
- PMSF
phenylmethylsulfonyl fluoride
- GMPCPP
guanosine-5-(,-methylene)triphosphate 相似文献
82.
Margaret E. Buckingham Arlette Cohen François Gros 《Journal of molecular biology》1976,103(3):611-626
Total poly(A)-containing RNA in different polysomal and supernatant cytoplasmic fractions was analysed after pulse-labelling in dividing myoblasts and fused myotubes. In particular, the peak of 26 S RNA (putative messenger for the large subunit of myosin) is located in a light region of the gradient coinciding with the monosome-trisome fractions prior to fusion, and is found in the heavy polysomes only after fusion. These heavy polysomes are free (i.e. not membrane bound). Treatment of the light part of the polysome gradient with EDTA shows that the 26 S RNA found here does not exist as part of a polysomal complex, but is present as a ribonucleoprotein particle cosedimenting in this region. Previous experiments had indicated that in actively dividing myoblasts 26 S RNA has a relatively short half-life but that it becomes “stable” after the cessation of mitosis just prior to fusion. RNA chase experiments performed in the present study show that the “short-lived” 26 S RNA from dividing myoblasts, which is present as a ribonucleoprotein particle, does not enter the heavy polysomes. In contrast, the more stable 26 S RNA also initially present as a ribonucleoprotein, just prior to and in the early stages of fusion, can be shown by chase experiments to enter the heavy polysomes later in fusion. Hence accumulation of 26 S RNA seems to precede its activation as a messenger. 相似文献
83.
Sergio Salardi Lucia Tosi Arlette Garnier-Suillerot Claudio Toniolo Gian Maria Bonora Fernando Marchiori 《Biopolymers》1982,21(6):1229-1243
The interaction of Cu(II) with the protamine clupeine YII (containing proline at the N-terminal) and with four peptides (H-Ala-Arg-OMe, H-Ala-Arg2-OMe, H-Pro-Arg-OMe, and H-Arg4-Tyr) has been studied by means of absorption, CD, and pH neasurements. The first two peptides mimic clupeine YI and Z N-terminals; the third, the clupeine YII N-terminal. At 1:1 molar ratio, clupeine YII yields two complexes: the first (I), at pH 6.6, through coordination via the N-terminal and the contiguous peptide nitrogen forming a five-membered chelate; the second (II), at pH 8.5, through the occupancy of the other two corners of the coordination square by amino nitrogens of the lateral chains. These complexes are strictly analogous and occur at the same pH as those formed with clupeine Z. Under the same conditions, all the peptides yield complex I in the first step, although the pH at which this complex is fully defined depends on the number of residues in the chain. It is 8.5 for dipeptides, decreases to 6.5 by the addition of a third residue to the chain, and remains constant when the number of residues is three or more. The amino nitrogens of lateral chains are unable to coordinate to the metal in a second step unless one additional peptide bond lies between the N-terminal residue and that containing the lateral chain bound to the metal. Thus, H-Ala-Arg-OMe and H-Pro-Arg-OMe form hydroxyl complexes in a second step (pH 11), by deprotonation of one of the water molecules coordinated to the metal; one of the lateral chains of H-Ala-Arg2-OMe is able to coordinate in a second step (pH 8.5), but it is only with H-Arg4-Tyr that a second complex (II) is obtained in which two amino nitrogens of lateral chains supersede the oxygens of water molecules in I, at pH 8.5. 相似文献
84.
The purpose of this work was to determine the conformation adopted in solution by adozelesin, carzelesin, and their derivatives obtained under basic or acidic conditions. Circular dichroism in the 270–330 nm wavelengths region was studied. In solution adozelesin can adopt two different conformations: a left-handed one which is thermodynamically favoured at low temperature and is mainly present at room temperature, and a right-handed one which is observed either at high temperature or at room temperature in the presence of DNA. This was ascertained by the presence of circular dichroism signals of the couplet type. The active form of carzelesin, i.e., U-76074, also exhibited a left-handed conformation in solution. Carzelesin and the derivatives obtained under acidic conditions that lack the cyclopropyl ring, and cyclopropylpyrroloindole, obtained under basic conditions, cannot adopt such a conformation. Chirality 8:585–589, 1996. © 1997 Wiley-Liss, Inc. 相似文献
85.
Alteration of the Leptin Network in Late Morbid Obesity Induced in Mice by Brain Infection with Canine Distemper Virus
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86.
Jean-Richard Neeser Arlette Chambaz Kim Yen Hoang Harriet Link-Amster 《FEMS microbiology letters》1988,49(2):301-307
Abstract Numerous structural families of naturally occurring glycopeptides and oligosaccharides have been evaluated as potential inhibitors of hemagglutinations mediated by CFA/I- and CFA/II-positive enterotoxigenic Escherichia coli strains. Among the preparations tested were glycopeptides with short O-linked (mucin-type) chains, various mixtures containing N-linked glycans (either oligomannoside-, hybrid- or complex-type), three fractions of human milk oligosaccharides, and glycopeptides derived from either pooled new-born meconiums or pooled human red blood cell membranes. In almost all cases, the same inhibitory preparations were active toward all E. coli strains. This emphasizes the close analogy between the carbohydrate specificities of the colonization factors concerned. Such inhibitors always contained lactosamine units in their oligosaccharide backbones, but this structural requirement alone was not sufficient for activity. The glycopeptide mixture derived from human erythrocyte membranes (known to contain blood group-related carbohydrate antigens carried by a lactosaminoglycan backbone) behaved as a potent hemagglutination inhibitor, especially towards CFA/II-expressing strains. This last result clearly indicates the structural family in which complex carbohydrates should be selected to establish precisely the specificity of these CFA/II adhesins. 相似文献
87.
Perry AC Wang X Feldman BB Ruth T Signorile J 《Journal of strength and conditioning research / National Strength & Conditioning Association》2004,18(1):136-143
The present study examined the impact of physical characteristics of adolescent competitive tennis players (13-18 years) on field tests of tennis performance. Results (n = 33) showed that boys were taller (p = 0.001), possessed greater wingspan (p = 0.030), had greater maximum oxygen consumption (p = 0.001), and performed better on isokinetic strength measurements. Multiple regression analyses controlling for age and sex showed that height (p = 0.025), maximum minute ventilation (p = 0.005), and isokinetic strength measurements significantly and positively affected ball velocity. Knee extension average power was the only variable to positively and significantly affect ball placement (p = 0.040); however, several isokinetic strength measurements negatively affected ball placement. For the nondominant side, down-the-line strokes showed reduced accuracy (p = 0.001) and ball velocity (p = 0.001) compared with cross-court strokes. Given our results, resistance training may be beneficial for the performance of on-court tennis play. 相似文献
88.
S. Rakotomanga A. Baillet F. Pellerin D. Baylocq-Ferrier 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1991,570(2):277-284
A reversed-phase liquid chromatographic assay was developed for the specific evaluation of metabolic by-products in the urine of galactosemic patients and based on the simultaneous determination of gluconolactone, galactonolactone and galactitol. The procedure involved a lyophilization step and the formation of phenylisocyanate derivatives, followed by injection directly into the chromatograph. Analytical results showed good selectivity, linearity, precision and accuracy. The method enabled the detection of levels as low as 0.05–0.1 ng, and compared favourably with other published techniques for the estimation of aldonic acids in biological fluids. 相似文献
89.
90.
Cristina Bocanegra Sara Gallego Jacobo Mendioroz Milagros Moreno Elena Sulleiro Fernando Salvador Nicolau Sikaleta Arlette Nindia Daniel Tchipita Morais Joromba Sebastiao Kavaya Adrián Sánchez Montalvá Teresa López Israel Molina 《PLoS neglected tropical diseases》2015,9(10)