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Renal and endocrine changes in rats with inherited stress-induced arterial hypertension (ISIAH) 总被引:2,自引:2,他引:0
Amstislavsky S Welker P Frühauf JH Maslova L Ivanova L Jensen B Markel AL Bachmann S 《Histochemistry and cell biology》2006,125(6):651-659
Hypertensive inbred rats (ISIAH; inherited stress-induced arterial hypertension) present with baseline hypertension (>170 mmHg in adult rats), but attain substantially higher values upon mild emotional stress. We aimed to characterize key parameters related to hypertension in ISIAH. Kidneys, adrenals, and systemic endocrine parameters were studied in ISIAH of different ages and compared to normotensive Wistar albino Glaxo (WAG) rats. Native organs were obtained for Western and PCR analysis. Perfusion-fixed organs were prepared for histopathology and quantitative histochemistry. Plasma renin and adrenal hormones were measured. Renal morphology was unaltered in ISIAH. The hypothalamic-pituitary-adrenocortical (HPA) axis was constitutively upregulated with enlarged adrenal cortices and enhanced plasma corticosterone levels. Plasma renin activity was not different between groups, whereas aldosterone levels were in part reduced. Juxtaglomerular NO synthase type 1, cyclooxygenase type 2, and renin expression were significantly reduced, whereas tubular gene products related to sodium transport (bumetanide-sensitive Na, K, 2Cl cotransporter type 2; thiazide-sensitive Na, Cl cotransporter; epithelial Na channel-α; 11β-hydroxysteroid dehydrogenase type 2) were increased. These data suggest enhanced volume conservation by the kidney. Our data define ISIAH as an attractive model for the renal components determining salt and water homeostasis in hypertension. The specific condition of a basally stimulated HPA axis is highlighted, including the option to study effects superimposed by emotional stress. 相似文献
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A Rutkovskiy LH Mariero S Nygård KO Stensløkken G Valen J Vaage 《Biochemical and biophysical research communications》2012,421(1):70-75
Angiotensin-I converting enzyme (ACE) is a zinc dependent peptidase with a major role in regulating vasoactive peptide metabolism. ACE, a transmembrane protein, undergoes proteolysis, or shedding, by an as yet unidentified proteinase to release a catalytically active soluble form of the enzyme. Physiologically, soluble ACE in plasma is derived primarily from endothelial cells. We demonstrate that ACE shedding from confluent endothelial cells is increased in response to bacterial lipopolysaccharide, but not phorbol esters. Characterisation of lipopolysaccharide stimulated shedding showed that there is a lag phase before soluble ACE can be detected which is sensitive to inhibitors of translation, NF-κB, TNFα and TNFR-I/II. The shedding phase is less sensitive to these inhibitors, but is ablated by BB-94, a Matrix Metalloproteinase (MMP)/A Disintegrin and Metalloproteinase (ADAM) inhibitor. Tissue Inhibitor of Metalloproteinase (TIMP) profiling suggested a requirement for ADAM9 in lipopolysaccharide induced ACE shedding, which was confirmed by depletion with siRNA. Transient transfection of ADAM9 and ACE cDNAs into HEK293 cells demonstrated that ADAM9 requires both membrane anchorage and its catalytic domain to shed ACE. 相似文献
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Denisenko Yury A. Korotkova Olga G. Zorov Ivan N. Rozhkova Alexandra M. Semenova Margarita V. Elcheninov Alexandr G. Kublanov Ilya V. Sinitsyn Arkady P. 《Biochemistry. Biokhimii?a》2021,86(4):489-495
Biochemistry (Moscow) - Heterologous endo-xanthanase (EX) from the thermophilic planktomycete Thermogutta terrifontis strain was obtained using Penicillium verruculosum 537 (ΔniaD) expression... 相似文献
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Vinokurov M Ostrov V Yurinskaya M Garbuz D Murashev A Antonova O Evgen'ev M 《Cell stress & chaperones》2012,17(1):89-101
It has been previously reported that pretreatment with exogenous heat shock protein 70 (Hsp70) is able to protect cells and
animals from the deleterious effects of bacterial lipopolysaccharide (LPS) produced by Gram-negative bacteria. However, the
effects of Hsp70 pretreatment on lipoteichoic acid (LTA) challenge resulted from Gram-positive bacteria infection have not
been fully elucidated. In this study, we demonstrated that preconditioning with human recombinant Hsp70 ameliorates various
manifestations of systematic inflammation, including reactive oxygen species, TNFα, and CD11b/CD18 adhesion receptor expression
induction observed in different myeloid cells after LTA addition. Therefore, exogenous Hsp70 may provide a mechanism for controlling
excessive inflammatory responses after macrophage activation. Furthermore, in a rat model of LTA-induced sepsis, we demonstrated
that prophylactic administration of exogenous human Hsp70 significantly exacerbated numerous homeostatic and hemodynamic disturbances
induced by LTA challenge and partially normalized the coagulation system and multiple biochemical blood parameters, including
albumin and bilirubin concentrations, which were severely disturbed after LTA injections. Importantly, prophylactic intravenous
injection of Hsp70 before LTA challenge significantly reduced mortality rates. Thus, exogenous mammalian Hsp70 may serve as
a powerful cellular defense agent against the deleterious effects of bacterial pathogens, such as LTA and LPS. Taken together,
our findings reveal novel functions of this protein and establish exogenous Hsp70 as a promising pharmacological agent for
the prophylactic treatment of various types of sepsis. 相似文献
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Chuanfei Wang Wei Zhang Xiangyi Meng Jonas Bergqvist Xianjie Liu Zewdneh Genene Xiaofeng Xu Arkady Yartsev Olle Inganäs Wei Ma Ergang Wang Mats Fahlman 《Liver Transplantation》2017,7(21)
A new strategy for designing ternary solar cells is reported in this paper. A low‐bandgap polymer named PTB7‐Th and a high‐bandgap polymer named PBDTTS‐FTAZ sharing the same bulk ionization potential and interface positive integer charge transfer energy while featuring complementary absorption spectra are selected. They are used to fabricate efficient ternary solar cells, where the hole can be transported freely between the two donor polymers and collected by the electrode as in one broadband low bandgap polymer. Furthermore, the fullerene acceptor is chosen so that the energy of the positive integer charge transfer state of the two donor polymers is equal to the energy of negative integer charge transfer state of the fullerene, enabling enhanced dissociation of all polymer donor and fullerene acceptor excitons and suppressed bimolecular and trap assistant recombination. The two donor polymers feature good miscibility and energy transfer from high‐bandgap polymer of PBDTTS‐FTAZ to low‐bandgap polymer of PTB7‐Th, which contribute to enhanced performance of the ternary solar cell. 相似文献
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Robert E Lintner Pankaj K Mishra Poonam Srivastava Betsy M Martinez-Vaz Arkady B Khodursky Robert M Blumenthal 《BMC microbiology》2008,8(1):60
Background
Bacterial genome sequences are being determined rapidly, but few species are physiologically well characterized. Predicting regulation from genome sequences usually involves extrapolation from better-studied bacteria, using the hypothesis that a conserved regulator, conserved target gene, and predicted regulator-binding site in the target promoter imply conserved regulation between the two species. However many compared organisms are ecologically and physiologically diverse, and the limits of extrapolation have not been well tested. In E. coli K-12 the leucine-responsive regulatory protein (Lrp) affects expression of ~400 genes. Proteus mirabilis and Vibrio cholerae have highly-conserved lrp orthologs (98% and 92% identity to E. coli lrp). The functional equivalence of Lrp from these related species was assessed. 相似文献69.
Volker F. Wendisch Daniel P. Zimmer Arkady Khodursky Brian Peter Nicholas Cozzarelli Sydney Kustu 《Analytical biochemistry》2001,290(2):205
Bacterial messenger RNA (mRNA) is not coherently polyadenylated, whereas mRNA of Eukarya can be separated from stable RNAs by virtue of polyadenylated 3′-termini. We have developed a method to isolate Escherichia coli mRNA by polyadenylating it in crude cell extracts with E. coli poly(A) polymerase I and purifying it by oligo(dT) chromatography. Differences in lacZRNA levels were similar with purified mRNA and total RNA in dot blot hydridizations for cultures grown with or without gratuitous induction of the lactose operon. More broadly, changes in gene expression upon induction were similar when cDNAs primed from mRNA or total RNA with random hexanucleotides were hydridized to DNA microarrays for the E. coli genome. Comparable signal intensities were obtained with only 1% as much oligo(dT)-purified mRNA as total RNA, and hence in vitro poly(A) tailing appears to be selective for mRNA. These and additional studies of genome-wide expression with DNA microarrays provide evidence that in vitro poly(A) tailing works universally for E. coli mRNAs. 相似文献
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