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81.
Marta Majchrzak Anna Krzyzanowska Anna B. Kubiak Arkadiusz Wojtasik Tomasz Wolkowicz Jolanta Szych Pawel Parniewski 《Molecular biology reports》2014,41(11):7121-7132
Salmonella enterica subsp. enterica comprises a number of serovars, many of which pose an epidemiological threat to humans and are a worldwide cause of morbidity and mortality. Most reported food infection outbreaks involve the serovars Salmonella Enteritidis and Salmonella Typhimurium. Rapid identification to determine the primary sources of the bacterial contamination is important to the improvement of public health. In recent years, many DNA-based techniques have been applied to genotype Salmonella. Herein, we report the use of a manual TRS-PCR approach for the differentiation of the Salmonella enterica subspecies enterica serovars in a single-tube assay. One hundred seventy Salmonella strains were examined in this work. These consisted of serovars S. Enteritidis, S. Typhimurium, S. Infantis, S. Virchow, S. Hadar, S. Newport and S. Anatum. Five of the TRS-primers, N6(GTG)4, N6(CAC)4, N6(CGG)4, N6(CCG)4 and N6(CTG)4, perfectly distinguished the S. Enteritidis and S. Typhimurium serovars, and the N6(GTG)4 primer additionally grouped the other five frequently isolated serovars. In our opinion, the TRS-PCR methodology could be recommended for a quick and simple DNA-based test for inter-serovar discrimination of Salmonella strains. 相似文献
82.
Inhibitor of Transfer Ribonucleic Acid Methylases in the Differentiating Slime Mold Dictyostelium discoideum 下载免费PDF全文
Eight hours after the onset of morphogenesis, an inhibitor of transfer ribonucleic acid methylases appears in differentiating Dictyostelium discoideum. The inhibitor is also present in spores. Fifty per cent of the inhibiting activity is lost upon heating at 100 C for 5 min; it is nondialyzable and sensitive to trypsin. 相似文献
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84.
Rab11-FIP3 is critical for the structural integrity of the endosomal recycling compartment 总被引:2,自引:0,他引:2
Horgan CP Oleksy A Zhdanov AV Lall PY White IJ Khan AR Futter CE McCaffrey JG McCaffrey MW 《Traffic (Copenhagen, Denmark)》2007,8(4):414-430
Rab11-FIP3 is an endosomal recycling compartment (ERC) protein that is implicated in the process of membrane delivery from the ERC to sites of membrane insertion during cell division. Here we report that Rab11-FIP3 is critical for the structural integrity of the ERC during interphase. We demonstrate that knockdown of Rab11-FIP3 and expression of a mutant of Rab11-FIP3 that is Rab11-binding deficient cause loss of all ERC-marker protein staining from the pericentrosomal region of A431 cells. Furthermore, we find that fluorophore-labelled transferrin cannot access the pericentrosomal region of cells in which Rab11-FIP3 function has been perturbed. We find that this Rab11-FIP3 function appears to be specific because expression of the equivalent Rab11-binding deficient mutant of Rab-coupling protein does not perturb ERC morphology. In addition, we find that other organelles such as sorting and late endosomes are unaffected by loss of Rab11-FIP3 function. Finally, we demonstrate the presence of an extensive coiled-coil region between residues 463 and 692 of Rab11-FIP3, which exists as a dimer in solution and is critical to support its function on the ERC. Together, these data indicate that Rab11-FIP3 is necessary for the structural integrity of the pericentrosomal ERC. 相似文献
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The past several years have witnessed the emergence of a new world of nucleic-acid-based architectures with highly predictable and programmable self-assembly properties. For almost two decades, DNA has been the primary material for nucleic acid nanoconstruction. More recently, the dramatic increase in RNA structural information led to the development of RNA architectonics, the scientific study of the principles of RNA architecture with the aim of constructing RNA nanostructures of any arbitrary size and shape. The remarkable modularity and the distinct but complementary nature of RNA and DNA nanomaterials are revealed by the various self-assembly strategies that aim to achieve control of the arrangement of matter at a nanoscale level. 相似文献
88.
Rafał Wcisło Witold Dzwinel David A. Yuen Arkadiusz Z. Dudek 《Journal of molecular modeling》2009,15(12):1517-1539
The dynamics of a growing tumor involving mechanical remodeling of healthy tissue and vasculature is neglected in most of
the existing tumor models. This is due to the lack of efficient computational framework allowing for simulation of mechanical
interactions. Meanwhile, just these interactions trigger critical changes in tumor growth dynamics and are responsible for
its volumetric and directional progression. We describe here a novel 3-D model of tumor growth, which combines particle dynamics
with cellular automata concept. The particles represent both tissue cells and fragments of the vascular network. They interact
with their closest neighbors via semi-harmonic central forces simulating mechanical resistance of the cell walls. The particle dynamics is governed by both
the Newtonian laws of motion and the cellular automata rules. These rules can represent cell life-cycle and other biological
interactions involving smaller spatio-temporal scales. We show that our complex automata, particle based model can reproduce
realistic 3-D dynamics of the entire system consisting of the tumor, normal tissue cells, blood vessels and blood flow. It
can explain phenomena such as the inward cell motion in avascular tumor, stabilization of tumor growth by the external pressure,
tumor vascularization due to the process of angiogenesis, trapping of healthy cells by invading tumor, and influence of external
(boundary) conditions on the direction of tumor progression. We conclude that the particle model can serve as a general framework
for designing advanced multiscale models of tumor dynamics and it is very competitive to the modeling approaches presented
before. 相似文献
89.
90.
A comparison of the in vitro antimicrobial activity of liposomes containing meropenem and gentamicin
Drulis-Kawa Z Gubernator J Dorotkiewicz-Jach A Doroszkiewicz W Kozubek A 《Cellular & molecular biology letters》2006,11(3):360-375
The antimicrobial activity of eight cationic, two neutral and three anionic liposome compositions containing meropenem and
gentamicin was tested in vitro in broth and serum medium. The cationic formulations showed better antibacterial efficacy against both Gram-positive and
Gram-negative bacteria than the anionic and neutral ones, regardless of the encapsulated drug. The most effective formulations
were the cationic PC/DOPE/DOTAP 3:4:3 and PC/Chol/DOTAP 3:4:3, as the MICs with meropenem were 2 to 4 times lower than those
of the free drug. 相似文献