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941.
942.
Three new coumarins, sesebrin(7-methoxy-5-[3-methylbut-2-enyloxy]-8-[3-methyl-2,3-epoxybutyl] coumarin), sesebrinol(7-methoxy-5-[3-methylbut-2-enyloxy]-8-[3-methyl-2,3-dihydroxybutyl] coumarin) and sibiricol (7-methoxy-5-hydroxy-8-[3-methylbut-2-enyl] coumarin) have been isolated from the roots of Seseli sibiricum, in addition to sitosterol and 7 known coumarins, isobergapten, bergapten, phellopterin, sibiricin, coumurrayin, osthenol and meranzin hydrate. 相似文献
943.
The mouse embryonic submandibular epithelium begins as a single bud from the floor of the mouth which, under the influence of its surrounding mesenchyme, grows and forms lobules that subsequently branch repetitively. The lobular morphology of the 13-day epithelium is maintained by its basal lamina which is a continuous layer on the interlobular clefts but is interrupted on the distal aspects of the lobules. The structural integrity of this lamina is dependent upon its glycosaminoglycan (GAG) which, by histochemistry, is more abundant on the interlobular clefts than on the distal lobules. We have investigated the basis for these regional differences in the lamina by examining the synthesis and degradation of total GAG as well as the accumulation and loss of laminar GAG during the morphogenesis of the 13-day gland. Autoradiography and histochemistry show that laminar GAG is rapidly turning over. Although it is relatively stable in the interlobular clefts, GAG is rapidly degraded on the distal lobules. This difference can account for the regional variation in basal laminar GAG accumulation. The results of incorporation kinetics and precursor pool specific activities of total epithelial GAG show that the rate of GAG synthesis is greater than its rate of degradation in the base of the lobules, which includes the interlobular clefts. In contrast, during morphogenesis, the rate of GAG degradation becomes greater than its rate of replacement in the distal lobules. The epithelial stalk appears to be in the steady state regarding GAG metabolism. We propose (a) that the rapid laminar GAG degradation on the distal lobules produces the interruptions in the lamina, allowing epithelial growth and expansion, and (b) that the metabolic stability of laminar GAG on the interlobular clefts maintains the integrity of this lamina which serves as a cellular constraint. The results are consistent with a model for epithelial morphogenesis in which the mesenchyme remodels the lamina, which in turn, dictates epithelial morphology. Regulation of basal lamina turnover may be a general mechanism for controlling the behavior of epithelial cell populations. 相似文献
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S K Banerjee E Holler G P Hess J A Rupley 《The Journal of biological chemistry》1975,250(11):4355-4367
Temperature jump and stopped flow methods were used to study at pH 7.0 the temperature dependence of elementary steps of the reactions of lysozyme with the beta(1 yields 4)-linked trimer, tetramer, and hexamer of N-acetylglucosamine. The steady state rate of cleavage of the hexasaccharide was determined as a function of temperature (5 degrees-40 degrees) and pH(2 to 8) in H-2O solution and as a function of pD(2.5 to 9.5) at 40 degrees in D-2O solution. The apparent enthalpies of the two ionizations of apparent pK 3.8 and 6.7 observed in measurements of k are 0 to 2 kcal/mol. The energy of activation determined for the pH optimum is 21.5 kcal/mol. The solvent deuterium isotope effect measured for k at the pH (pD) optimum is 1.5 And reflects isotope effects on pre-equilibrium steps and on the rate-determining step. Transfer from H-2O to D-2O solution produces 0.2 to 0.4 kcal/mol more negative free energies of saccharide binding and no changes in the enthalpies of binding. Pre-steady state, steady state, and equilibrium measurements indicate a pathway for the reaction of lysozyme with hexasaccharide. The results define for this mechanism the complete free energy profile and an essentially complete enthalpy profile. Three of the five observable ES complexes are present at nearly equal concentrations. The free energies of the transition states are within a range of 3 kcal. The enthalpies of productive enzyme-substrate complexes are about 5 kcal/mol greater than the enthalpies of nonproductive complexes. Changes in tryptophan fluorescence were observed for each elementary step, and changes in pK of Glu-35 for the isomerizations of nonproductive and productive complexes. The signal changes during formation of nonproductive complexes are the same for the oligosaccharides (ClcNAc)3 to (GlcNAc)6. The changes for productive complexes are similar but not identical with saccharides (GlcNAc)4 to (GlcNAc)6. Correlations of the present data with previous crystallographic and solution measurements indicate the structures of productive and nonproductive ES complexes and suggest that full interaction of the substrate with the enzyme active site is established in the rate-determining step. 相似文献
950.
Myosin from the hearts of thyrotoxic animals (myosin-T) exhibits elevated Ca2+-ATPase activity. To clarify the physiological significance of this increased activity, we have investigated the steady state kinetics of the interaction of actin and MgATP with the double-headed heavy meromyosin subfragment of cardiac myosin from thyrotoxic rabbits (HMM-T). The enhanced Ca2+-ATPase activity of myosin-T was completely retained in HMM-T. The Vmax for actin-activated MgATP hydrolysis by HMM-T (1.08 +/- 0.10 mumol of Pi/mg/min). Under physiological ionic conditions, the Vmax was 0.14 +/- 0.02 mumol of Pi/mg/min as compared with the normal value of 0.08 +/- 0.01 mumol of Pi/mg/min. Furthermore, the salt dependence of Vmax and Kapp for the actin-activated ATPase of HMM-T differed markedly from normal and resembled that usually associated with the single-headed (S1) cleavage product of myosin. These results suggest that the changes in enzymatic properties of myosin-T are responsible for the increased speed of contraction observed in the hearts of thyrotoxic animals. Also, the alteration in the interaction of HMM-T with actin suggests that a loss of cooperativity between the myosin heads may occur. 相似文献