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991.
Tae-Young Hwang Takashi Sayama Masakazu Takahashi Yoshitake Takada Yumi Nakamoto Hideyuki Funatsuki Hiroshi Hisano Shigemi Sasamoto Shusei Sato Satoshi Tabata Izumi Kono Masako Hoshi Masayoshi Hanawa Chizuru Yano Zhengjun Xia Kyuya Harada Keisuke Kitamura Masao Ishimoto 《DNA research》2009,16(4):213-225
A well-saturated molecular linkage map is a prerequisite for modern plant breeding. Several genetic maps have been developed for soybean with various types of molecular markers. Simple sequence repeats (SSRs) are single-locus markers with high allelic variation and are widely applicable to different genotypes. We have now mapped 1810 SSR or sequence-tagged site markers in one or more of three recombinant inbred populations of soybean (the US cultivar ‘Jack’ × the Japanese cultivar ‘Fukuyutaka’, the Chinese cultivar ‘Peking’ × the Japanese cultivar ‘Akita’, and the Japanese cultivar ‘Misuzudaizu’ × the Chinese breeding line ‘Moshidou Gong 503’) and have aligned these markers with the 20 consensus linkage groups (LGs). The total length of the integrated linkage map was 2442.9 cM, and the average number of molecular markers was 90.5 (range of 70–114) for the 20 LGs. We examined allelic diversity for 1238 of the SSR markers among 23 soybean cultivars or lines and a wild accession. The number of alleles per locus ranged from 2 to 7, with an average of 2.8. Our high-density linkage map should facilitate ongoing and future genomic research such as analysis of quantitative trait loci and positional cloning in addition to marker-assisted selection in soybean breeding.Key words: EST-derived SSR marker, integrated linkage map, microsatellite marker, polymorphism information content 相似文献
992.
The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes 总被引:47,自引:0,他引:47
Zi-Xuan Wang Masahiro Yano Utako Yamanouchi > Masao Iwamoto Lisa Monna Hiroshi Hayasaka Yuichi Katayose Takuji Sasaki 《The Plant journal : for cell and molecular biology》1999,19(1):55-64
Rice blast, caused by the fungal pathogen Magnaporthe grisea, is one of the most serious diseases of rice. Here we describe the isolation and characterization of Pib, one of the rice blast resistance genes. The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness. 相似文献
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Hideo Ochiai Hitoshi Shibata Tetsu Matsuo Kenji Hashinokuchi Masao Yukawa 《Bioscience, biotechnology, and biochemistry》2013,77(4):721-722
Highly branched α-glucan molecules exhibit low digestibility for α-amylase and glucoamylase, and abundant in α-(1→3)-, α-(1→6)-glucosidic linkages and α-(1→6)-linked branch points where another glucosyl chain is initiated through an α-(1→3)-linkage. From a culture supernatant of Paenibacillus sp. PP710, we purified α-glucosidase (AGL) and α-amylase (AMY), which were involved in the production of highly branched α-glucan from maltodextrin. AGL catalyzed the transglucosylation reaction of a glucosyl residue to a nonreducing-end glucosyl residue by α-1,6-, α-1,4-, and α-1,3-linkages. AMY catalyzed the hydrolysis of the α-1,4-linkage and the intermolecular or intramolecular transfer of maltooligosaccharide like cyclodextrin glucanotransferase (CGTase). It also catalyzed the transfer of an α-1,4-glucosyl chain to a C3- or C4-hydroxyl group in the α-1,4- or α-1,6-linked nonreducing-end residue or the α-1,6-linked residue located in the other chains. Hence AMY was regarded as a novel enzyme. We think that the mechanism of formation of highly branched α-glucan from maltodextrin is as follows: α-1,6- and α-1,3-linked residues are generated by the transglucosylation of AGL at the nonreducing ends of glucosyl chains. Then AMY catalyzes the transfer of α-1,4-chains to C3- or C4-hydroxyl groups in the α-1,4- or α-1,6-linked residues generated by AGL. Thus the concerted reactions of both AGL and AMY are necessary to produce the highly branched α-glucan from maltodextrin. 相似文献
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997.
Hidehito Urata Masayuki Urata Masao Akagi Hidekazu Hiroaki Seiich Uesugi 《Nucleosides, nucleotides & nucleic acids》2013,32(6-7):1259-1269
Abstract We investigated the structure of a decadeoxynucleotide duplex which was modified with Dichloroethylenediamineplatinum(II) (PtCl2(en)) at the central GG site by using some spectroscopic techniques. The results suggest that the DNA structural changes induced by binding of PtCl2(en) are quite similar to those of cisplatin. 相似文献
998.
Sera Toshihiro Kobayashi Hiroaki Hoshino Masato Uesugi Kentaro Matsumoto Takeshi Tanaka Masao 《Biomechanics and modeling in mechanobiology》2019,18(2):375-385
Biomechanics and Modeling in Mechanobiology - In this study, based on the measurements of intracortical vascular canal structure, we investigated the disuse effect on local O2 supply in the... 相似文献
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