全文获取类型
收费全文 | 622篇 |
免费 | 71篇 |
出版年
2022年 | 3篇 |
2021年 | 10篇 |
2020年 | 6篇 |
2019年 | 12篇 |
2018年 | 9篇 |
2017年 | 8篇 |
2016年 | 19篇 |
2015年 | 33篇 |
2014年 | 32篇 |
2013年 | 41篇 |
2012年 | 41篇 |
2011年 | 35篇 |
2010年 | 22篇 |
2009年 | 28篇 |
2008年 | 18篇 |
2007年 | 38篇 |
2006年 | 42篇 |
2005年 | 31篇 |
2004年 | 25篇 |
2003年 | 24篇 |
2002年 | 20篇 |
2001年 | 25篇 |
2000年 | 19篇 |
1999年 | 21篇 |
1998年 | 5篇 |
1997年 | 6篇 |
1996年 | 12篇 |
1995年 | 7篇 |
1994年 | 4篇 |
1993年 | 5篇 |
1992年 | 5篇 |
1991年 | 14篇 |
1990年 | 8篇 |
1989年 | 3篇 |
1988年 | 8篇 |
1987年 | 4篇 |
1986年 | 12篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1982年 | 6篇 |
1981年 | 3篇 |
1980年 | 2篇 |
1978年 | 2篇 |
1977年 | 3篇 |
1975年 | 3篇 |
1974年 | 3篇 |
1973年 | 1篇 |
1972年 | 1篇 |
1971年 | 3篇 |
1966年 | 4篇 |
排序方式: 共有693条查询结果,搜索用时 31 毫秒
51.
52.
53.
Studies of several bird species have shown that coloured leg bands may affect a male's success in mate attraction and/or mating competition. From a colour band experiment in the field, we have previously reported that male bluethroats, Luscinia s. svecica, with blue and orange bands (BO males) guarded their mates less intensely at the peak of female fertility, and spent more time advertising for additional mates, than males banded with non-BO colours. These responses indicated that BO males experienced less threat to their paternity than did non-BO males, possibly mediated through an increased attractiveness. Here we present paternity analyses of the broods from the field study and test whether there were differences between the two male groups in within-pair or extrapair paternity. There were no significant differences between the two groups of males in paternity, suggesting effective male protection of paternity. However, extrapair paternity was infrequent in the 2 years of the field experiment; hence, the power in detecting effects on paternity does not allow a definitive conclusion on this issue. We also conducted an aviary experiment in which females were given the choice between a BO male and a non-BO male, to test whether females had preferences for particular colour bands. Females did not associate more with BO males, as would have been expected if these males were more attractive in social mate choice. Our results suggest that the effects of colour bands on social mate choice and paternity are, at best, weak. Copyright 2000 The Association for the Study of Animal Behaviour. 相似文献
54.
Johnsen AR Hausner M Schnell A Wuertz S 《Applied and environmental microbiology》2000,66(8):3487-3491
Three strains of Sphingomonas were grown as biofilms and tested for binding of five fluorescently labeled lectins (Con A-type IV-TRITC or -Cy5, Pha-E-TRITC, PNA-TRITC, UEA 1-TRITC, and WGA-Texas red). Only ConA and WGA were significantly bound by the biofilms. Binding of the five lectins to artificial biofilms made of the commercially available Sphingomonas extracellular polysaccharides was similar to binding to living biofilms. Staining of the living and artificial biofilms by ConA might be explained as binding of the lectin to the terminal mannosyl and terminal glucosyl residues in the polysaccharides secreted by Sphingomonas as well as to the terminal mannosyl residue in glycosphingolipids. Staining of the biofilms by WGA could only be explained as binding to the Sphingomonas glycosphingolipid membrane, binding to the cell wall, or nonspecific binding. Glycoconjugation of ConA and WGA with the target sugars glucose and N-acetylglucosamine, respectively, was used as a method for evaluation of the specificity of the lectins towards Sphingomonas biofilms and Sphingomonas polysaccharides. Our results show that the binding of lectins to biofilms does not necessarily prove the presence of specific target sugars in the extracellular polymeric substances (EPS) in biofilms. The lectins may bind to non-EPS targets or adhere nonspecifically to components of the biofilm matrix. 相似文献
55.
Philipp Rausch Natalie Steck Abdulhadi Suwandi Janice A. Seidel Sven Künzel Kirandeep Bhullar Marijana Basic Andre Bleich Jill M. Johnsen Bruce A. Vallance John F. Baines Guntram A. Grassl 《PLoS pathogens》2015,11(7)
Glycans play important roles in host-microbe interactions. Tissue-specific expression patterns of the blood group glycosyltransferase β-1,4-N-acetylgalactosaminyltransferase 2 (B4galnt2) are variable in wild mouse populations, and loss of B4galnt2 expression is associated with altered intestinal microbiota. We hypothesized that variation in B4galnt2 expression alters susceptibility to intestinal pathogens. To test this, we challenged mice genetically engineered to express different B4galnt2 tissue-specific patterns with a Salmonella Typhimurium infection model. We found B4galnt2 intestinal expression was strongly associated with bacterial community composition and increased Salmonella susceptibility as evidenced by increased intestinal inflammatory cytokines and infiltrating immune cells. Fecal transfer experiments demonstrated a crucial role of the B4galnt2-dependent microbiota in conferring susceptibility to intestinal inflammation, while epithelial B4galnt2 expression facilitated epithelial invasion of S. Typhimurium. These data support a critical role for B4galnt2 in gastrointestinal infections. We speculate that B4galnt2-specific differences in host susceptibility to intestinal pathogens underlie the strong signatures of balancing selection observed at the B4galnt2 locus in wild mouse populations. 相似文献
56.
57.
Tore Skrøppa Mari Mette Tollefsrud Christoph Sperisen Øystein Johnsen 《Tree Genetics & Genomes》2010,6(1):93-99
Seedlings of open-pollinated Picea abies families from Norwegian and Central European parent trees standing at three sites in Norway were tested for timing of bud
set at the end of the first growth season together with seedlings from control provenances producing seeds at their geographical
origin. The parental origins were confirmed with a maternally inherited mitochondrial marker that distinguishes trees of the
Northern European range from those of the Central European range. The seedlings from the families of Central European mother
trees producing seeds in Norway had on average a bud set more similar to the families of local Norwegian origin producing
seeds at the same site than the provenance of the same Central European origin. It is argued that the rapid change in this
adaptive trait from one generation to the next can be explained by recent research results demonstrating that day length and
temperature conditions during embryo formation and maturation can influence the phenotypic performance of seedlings in Norway
spruce. This effect may influence the fitness of naturally regenerated plants produced in plantations of Central European
trees in Norway. 相似文献
58.
Summary Modified B
S translocation males were developed at 26.0° C where univalentbearing gametes are recovered with less than half the frequency than at 18.0° C. Upon eclosion the males were stored for definite time periods at either temperature before mating individually to single y free-X females. the transfer cultures of the females show a higher frequency of recovery of univalent-bearing progeny regardless of the temperature or storage treatment of the male. In addition, postmeiotic temperature treatment does not appear to fundamentally alter the overall frequency of recovery of univalent-bearing gametes which is presumably determined by the developmental temperature of the male. A similar trend is observed for matings of y females to single X.YSYL/O males in which the males were developed and stored at 26.0° C; namely, a higher frequency of recovery of attached-XY gametes in the transfer cultures. 相似文献
59.
60.
Avian Clock gene polymorphism: evidence for a latitudinal cline in allele frequencies 总被引:2,自引:0,他引:2
Johnsen A Fidler AE Kuhn S Carter KL Hoffmann A Barr IR Biard C Charmantier A Eens M Korsten P Siitari H Tomiuk J Kempenaers B 《Molecular ecology》2007,16(22):4867-4880
In comparison with most animal behaviours, circadian rhythms have a well-characterized molecular genetic basis. Detailed studies of circadian clock genes in 'model' organisms provide a foundation for interpreting the functional and evolutionary significance of polymorphic circadian clock genes found within free-living animal populations. Here, we describe allelic variation in a region of the avian Clock orthologue which encodes a functionally significant polyglutamine repeat (ClkpolyQcds), within free-living populations of two passerine birds, the migratory bluethroat (Luscinia svecica) and the predominantly nonmigratory blue tit (Cyanistes caeruleus). Multiple ClkpolyQcds alleles were found within populations of both species (bluethroat: 12 populations, 7 alleles; blue tit: 14 populations, 9 alleles). Some populations of both species were differentiated at the ClkpolyQcds locus as measured by F(ST) and R(ST) values. Among the blue tit, but not bluethroat populations, we found evidence of latitudinal clines in (i) mean ClkpolyQcds repeat length, and (ii) the proportions of three ClkpolyQcds genotype groupings. Parallel analyses of microsatellite allele frequencies, which are considered to reflect selectively neutral processes, indicate that interpopulation allele frequency variation at the ClkpolyQcds and microsatellite loci does not reflect the same underlying demographic processes. The possibility that the observed interpopulation ClkpolyQcds allele frequency variation is, at least in part, maintained by selection for microevolutionary adaptation to photoperiodic parameters correlated with latitude warrants further study. 相似文献