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161.
Aureo Banhos Bruno L. Fontes Débora Regina Yogui Mario Henrique Alves Natália Carneiro Ardente Renata Valls Lucas Mendes Barreto Lucas Damásio Átilla Colombo Ferreguetti Andréa Siqueira Carvalho Vitor Roberto Schettino Alexandre Rosa dos Santos Helena Godoy Bergallo Ana Carolina Srbek-Araujo Emilia Patrícia Medici Ariel Canena Arnaud L.J. Desbiez 《Biotropica》2020,52(3):421-426
We report 24 records of giant armadillo roadkill on Brazilian highways in the Cerrado, Pantanal and Amazon biomes illustrating that highways are a threat to this species. However, we also documented the species using underpasses, demonstrating that these structures could help to reduce the risk of roadkill for giant armadillos. 相似文献
162.
Gonzalo E. Yevenes Gustavo Moraga-Cid Ariel Avila Leonardo Guzmán Maximiliano Figueroa Robert W. Peoples Luis G. Aguayo 《The Journal of biological chemistry》2010,285(39):30203-30213
It is now believed that the allosteric modulation produced by ethanol in glycine receptors (GlyRs) depends on alcohol binding to discrete sites within the protein structure. Thus, the differential ethanol sensitivity of diverse GlyR isoforms and mutants was explained by the presence of specific residues in putative alcohol pockets. Here, we demonstrate that ethanol sensitivity in two ligand-gated ion receptor members, the GlyR adult α1 and embryonic α2 subunits, can be modified through selective mutations that rescued or impaired Gβγ modulation. Even though both isoforms were able to physically interact with Gβγ, only the α1 GlyR was functionally modulated by Gβγ and pharmacological ethanol concentrations. Remarkably, the simultaneous switching of two transmembrane and a single extracellular residue in α2 GlyRs was enough to generate GlyRs modulated by Gβγ and low ethanol concentrations. Interestingly, although we found that these TM residues were different to those in the alcohol binding site, the extracellular residue was recently implicated in conformational changes important to generate a pre-open-activated state that precedes ion channel gating. Thus, these results support the idea that the differential ethanol sensitivity of these two GlyR isoforms rests on conformational changes in transmembrane and extracellular residues within the ion channel structure rather than in differences in alcohol binding pockets. Our results describe the molecular basis for the differential ethanol sensitivity of two ligand-gated ion receptor members based on selective Gβγ modulation and provide a new mechanistic framework for allosteric modulations of abuse drugs. 相似文献
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Lucio Aliperti Ariel A. Aptekmann Gonzalo Farfañuk Luciana L. Couso Alfonso Soler-Bistué Ignacio E. Sánchez 《Environmental microbiology》2023,25(12):3255-3268
The guanine/cytosine (GC) content of prokaryotic genomes is species-specific, taking values from 16% to 77%. This diversity of selection for GC content remains contentious. We analyse the correlations between GC content and a range of phenotypic and genotypic data in thousands of prokaryotes. GC content integrates well with these traits into r/K selection theory when phenotypic plasticity is considered. High GC-content prokaryotes are r-strategists with cheaper descendants thanks to a lower average amino acid metabolic cost, colonize unstable environments thanks to flagella and a bacillus form and are generalists in terms of resource opportunism and their defence mechanisms. Low GC content prokaryotes are K-strategists specialized for stable environments that maintain homeostasis via a high-cost outer cell membrane and endospore formation as a response to nutrient deprivation, and attain a higher nutrient-to-biomass yield. The lower proteome cost of high GC content prokaryotes is driven by the association between GC-rich codons and cheaper amino acids in the genetic code, while the correlation between GC content and genome size may be partly due to functional diversity driven by r/K selection. In all, molecular diversity in the GC content of prokaryotes may be a consequence of ecological r/K selection. 相似文献
166.
Gabriele Midolo Tomáš Herben Irena Axmanová Corrado Marcenò Ricarda Pätsch Helge Bruelheide Dirk Nikolaus Karger Svetlana Aćić Ariel Bergamini Erwin Bergmeier Idoia Biurrun Gianmaria Bonari Andraž Čarni Alessandro Chiarucci Michele De Sanctis Olga Demina Jürgen Dengler Tetiana Dziuba Giuliano Fanelli Emmanuel Garbolino Gianpietro Giusso del Galdo Friedemann Goral Behlül Güler Guillermo Hinojos-Mendoza Florian Jansen Borja Jiménez-Alfaro Attila Lengyel Jonathan Lenoir Aaron Pérez-Haase Remigiusz Pielech Vadim Prokhorov Valerijus Rašomavičius Eszter Ruprecht Solvita Rūsiņa Urban Šilc Željko Škvorc Zvjezdana Stančić Irina Tatarenko Milan Chytrý 《Global Ecology and Biogeography》2023,32(1):24-34
167.
The hallmark of obesity and one of the key contributing factors to insulin resistance, type 2 diabetes and cardiovascular disease is excess triacylglycerol (TG) storage. In hepatocytes, excessive accumulation of TG is the common denominator of a wide range of clinicopathological entities known as nonalcoholic fatty liver disease, which can eventually progress to cirrhosis and associated complications including hepatic failure, hepatocellular carcinoma and death. A tight regulation between TG synthesis, hydrolysis, secretion and fatty acid oxidation is required to prevent lipid accumulation as well as lipid depletion from hepatocytes. Therefore, understanding the pathways that regulate hepatic TG metabolism is crucial for development of therapies to ameliorate pathophysiological conditions associated with excessive hepatic TG accumulation, including dyslipidemias, viral infection and atherosclerosis. This review highlights the physiological roles of liver lipases that degrade TG in cytosolic lipid droplets, endoplasmic reticulum, late endosomes/lysosomes and along the secretory route. This article is part of a Special Issue entitled Triglyceride Metabolism and Disease. 相似文献
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Hashlamoun Kotaybah Abusara Ziad Ramírez-Torres Ariel Grillo Alfio Herzog Walter Federico Salvatore 《Biomechanics and modeling in mechanobiology》2020,19(6):2397-2412
Biomechanics and Modeling in Mechanobiology - Fluorescence recovery after photobleaching (FRAP) is a widely used technique for studying diffusion in biological tissues. Most of the existing... 相似文献
170.
Different Pathways Act Downstream of the CEP Peptide Receptor CRA2 to Regulate Lateral Root and Nodule Development 总被引:1,自引:0,他引:1