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341.
342.
Stefan?LarssonEmail author Tommi?Linnansaari Sauli?Vatanen Ignacio?Serrano Ari?Haikonen 《Environmental Biology of Fishes》2011,92(3):361-369
In general, hatchery salmonid smolts experience higher mortality during migration than wild smolts, which is suggested to
be due to domestication effects and that hatchery fish lack experience of the natural environment. However, possible differences
in feeding during smolt migration between hatchery and wild smolts have rarely been addressed. We compared the number of feeding
smolts and stomach fullness among wild Atlantic salmon smolts, hatchery-reared smolts released as 1-year-old parr, and hatchery-reared
smolts released as 2-year-old smolts during their descent to sea in River Tornionjoki. In addition, estimations of prey selection
among the smolt groups were conducted. A high proportion of wild smolts and smolts stocked as parr actively fed during the
smolt migration. A lower proportion of smolts stocked as smolts was feeding and their stomach fullness were much reduced in
comparison with the two other groups. The study also indicated that the feeding of migrating smolts is selective rather than
opportunistic. In conclusion, this study suggests that stocked 2-year-old smolts may enter sea with an inferior foraging behaviour
and it is a possibility that this may contribute to the observed low post-smolt survival in the Baltic Sea. 相似文献
343.
Jumpponen A 《Mycorrhiza》2011,21(6):453-464
Use of the reverse-transcribed small subunit of the ribosomal RNA (rRNA) was tested for exploring seasonal dynamics of fungal
communities associated with the roots of the dominant tallgrass prairie grass, Andropogon gerardii. Ribosomal RNA was extracted, reverse-transcribed, and PCR-amplified in four sampling events in May, July, September, and
November. Analyses of cloned PCR amplicons indicated that the A. gerardii rhizospheres host phylogenetically diverse fungal communities and that these communities are seasonally dynamic. Operational
taxonomic units with Basic Local Alignment Search Tool affinities within the order Helotiales were dominant in the rhizosphere
in May. These putative saprobes were largely replaced by arbuscular mycorrhizal fungi with likely affinities within Glomerales
suggesting that the fungal communities are not only compositionally but also functionally dynamic. These data suggest replacement
of functional guilds comprised of saprobic fungi by mutualistic fungi in the course of a growing season. 相似文献
344.
Neural encoding of sensory signals involves both linear and nonlinear processes. Determining which nonlinear operations are implemented by neural systems is crucial to understanding sensory processing. Here, we ask if demodulation, the process used to decode AM radio signals, describes how Y cells in the cat LGN nonlinearly encode the visual scene. In response to visual AM signals across?a wide range of carrier frequencies, Y cells were found to transmit a demodulated signal, with the firing rate of single-units fluctuating at the envelope frequency but not the carrier frequency. A comparison of temporal frequency tuning properties between LGN Y cells and neurons in two primary cortical areas suggests that Y cells initiate a distinct pathway that carries a demodulated representation of the visual scene to cortex. The nonlinear signal processing carried out by the Y cell pathway simplifies the neural representation of complex visual features and allows high spatiotemporal frequencies to drive cortical responses. 相似文献
345.
346.
Calreticulin is a soluble calcium-binding chaperone of the endoplasmic reticulum (ER) that is also detected on the cell surface and in the cytosol. Calreticulin contains a single high affinity calcium-binding site within a globular domain and multiple low affinity sites within a C-terminal acidic region. We show that the secondary structure of calreticulin is remarkably thermostable at a given calcium concentration. Rather than corresponding to complete unfolding events, heat-induced structural transitions observed for calreticulin relate to tertiary structural changes that expose hydrophobic residues and reduce protein rigidity. The thermostability and the overall secondary structure content of calreticulin are impacted by the divalent cation environment, with the ER range of calcium concentrations enhancing stability, and calcium-depleting or high calcium environments reducing stability. Furthermore, magnesium competes with calcium for binding to calreticulin and reduces thermostability. The acidic domain of calreticulin is an important mediator of calcium-dependent changes in secondary structure content and thermostability. Together, these studies indicate interactions between the globular and acidic domains of calreticulin that are impacted by divalent cations. These interactions influence the structure and stability of calreticulin, and are likely to determine the multiple functional activities of calreticulin in different subcellular environments. 相似文献
347.
Dixit N Yamayoshi I Nazarian A Simon SI 《Journal of immunology (Baltimore, Md. : 1950)》2011,187(1):472-481
Acute inflammation triggers the innate immune response of neutrophils that efficiently traffic from the bloodstream to concentrate at high numbers at the site of tissue infection or wounding. A gatekeeper in this process is activation of β(2) integrins, which form bond clusters with ICAM-1 on the endothelial surface. These bond clusters serve dual functions of providing adhesive strength to anchor neutrophils under the shear forces of blood flow and directional guidance for cell polarization and subsequent transmigration on inflamed endothelium. We hypothesized that shear forces transmitted through high-affinity LFA-1 facilitates the cooperation with the calcium release-activated channel Orai1 in directing localized cytoskeletal activation and directed migration. By using vascular mimetic microfluidic channels, we observed neutrophil arrest on a substrate of either ICAM-1 or allosteric Abs that stabilize a high- or low-affinity conformation of LFA-1. Neutrophils captured via low-affinity LFA-1 did not exhibit intracellular calcium flux, F-actin polymerization, cell polarization, or directional migration under shear flow. In contrast, high-affinity LFA-1 provided orientation along a uropod-pseudopod axis that required calcium flux through Orai1. We demonstrate how the shear stress of blood flow can transduce distinct outside-in signals at focal sites of high-affinity LFA-1 that provide contact-mediated guidance for neutrophil emigration. 相似文献
348.
Development of ground vegetation biomass and nutrient pools in a clear-cut disc-plowed boreal forest
Marjo Palviainen Leena Finér Ari Laurén Hannu Mannerkoski Sirpa Piirainen Michael Starr 《Plant and Soil》2007,297(1-2):43-52
Nutrient leaching from forest substrate after clear-cutting and subsequent soil preparation is strongly influenced by the
capacity of ground vegetation to sequester the released nutrients. We studied the rates and patterns of biomass and nutrient
accumulation in ground vegetation growing on ridges, in furrows and on undisturbed surfaces for 2–5 years after disc-plowing
in eastern Finland. The biomass of mosses on ridges remained significantly lower than that in furrows and on undisturbed surfaces.
Field layer biomass on ridges and in furrows was significantly lower than on undisturbed surfaces throughout the study period.
Field layer biomass increased more on ridges than in furrows. Root biomass on ridges and undisturbed surfaces was considerably
higher than in furrows. Five years after disc-plowing, total biomass and nutrient pools for ridges (biomass 4,975 kg ha−1, N 40 kg ha−1, P 5 kg ha−1, K 20 kg ha−1 and Ca 18 kg ha−1) and undisturbed surfaces (biomass 5,613 kg ha−1, N 43 kg ha−1, P 5 kg ha−1, K 22 kg ha−1 and Ca 18 kg ha−1) were similar, but considerably lower for furrows (biomass 1,807 kg ha−1, N 16 kg ha−1, P 2 kg ha−1, K 10 kg ha−1 and Ca 6 kg ha−1). Ridges covered 25% of the area, furrows 30 and 45% was undisturbed surfaces. Taking into account the proportion of each
type of surface, values for the whole prepared clear-cut area were 4,312, 34, 4, 18 and 14 kg ha−1 for biomass, N, P, K and Ca, respectively. Biomass and nutrient pools had not returned to uncut forest levels at the end
of the 5-year study period. The results indicate that mosses and field layer vegetation respond differently to soil preparation,
that the development of biomass on ridges, in furrows and on undisturbed surfaces proceeds at different rates, and that the
biomass and nutrient uptake of ground vegetation remains below pre-site preparation levels for several years. However, ridges,
which are known to be the most susceptible to leaching, revegetate rapidly.
Responsible Editor: Tibor Kalapos. 相似文献
349.
Ostrow D Phillips N Avalos A Blanton D Boggs A Keller T Levy L Rosenbloom J Baer CF 《Genetics》2007,176(3):1653-1661
Mutational bias is a potentially important agent of evolution, but it is difficult to disentangle the effects of mutation from those of natural selection. Mutation-accumulation experiments, in which mutations are allowed to accumulate at very small population size, thus minimizing the efficiency of natural selection, are the best way to separate the effects of mutation from those of selection. Body size varies greatly among species of nematode in the family rhabditidae; mutational biases are both a potential cause and a consequence of that variation. We report data on the cumulative effects of mutations that affect body size in three species of rhabditid nematode that vary fivefold in adult size. Results are very consistent with previous studies of mutations underlying fitness in the same strains: two strains of Caenorhabditis briggsae decline in body size about twice as fast as two strains of C. elegans, with a concomitant higher point estimate of the genomic mutation rate; the confamilial Oscheius myriophila is intermediate. There is an overall mutational bias, such that mutations reduce size on average, but the bias appears consistent between species. The genetic correlation between mutations that affect size and those underlying fitness is large and positive, on average. 相似文献
350.
Ojala JR Pikkarainen T Tuuttila A Sandalova T Tryggvason K 《The Journal of biological chemistry》2007,282(22):16654-16666
MARCO is a trimeric class A scavenger receptor of macrophages and dendritic cells that recognizes polyanionic particles and pathogens. The distal, scavenger receptor cysteine-rich (SRCR) domain of the extracellular part of this receptor has been implicated in ligand binding. To provide a structural basis for understanding the ligand-binding mechanisms of MARCO, we have determined the crystal structure of the mouse MARCO SRCR domain. The recombinant SRCR domain purified as monomeric and dimeric forms, and their structures were determined at 1.78 and 1.77 A resolution, respectively. The monomer has a compact globular fold with a twisted five-stranded antiparallel beta-sheet and a long loop covering a single alpha-helix, whereas the dimer is formed via beta-strand swapping of two monomers, thus containing a large eight-stranded beta-sheet. Calculation of the surface electrostatic potential revealed that the beta-sheet region with several arginines forms a basic cluster. Unexpectedly, an acidic cluster was found in the long loop region. In the monomer, the acidic cluster is involved in metal ion binding. Studies with cells expressing various SRCR domain mutants showed that all of the arginines of the basic cluster are involved in ligand binding, suggesting a cooperative binding mechanism. Ligand binding is also dependent on the acidic cluster and Ca2+ ions whose depletion appears to affect ligand binding at least by modulating the electrostatic potential or relative domain orientation. We propose that the SRCR domain dimerization can contribute to the recognition of large ligands by providing a means for the MARCO receptor oligomerization. 相似文献