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241.
Hannah B. Fried‐Petersen Yimen G. Araya‐Ajoy Martyn N. Futter David G. Angeler 《Global Change Biology》2020,26(3):1259-1270
Research on ecosystem stability has had a strong focus on local systems. However, environmental change often occurs slowly at broad spatial scales, which requires regional‐level assessments of long‐term stability. In this study, we assess the stability of macroinvertebrate communities across 105 lakes in the Swedish “lakescape.” Using a hierarchical mixed‐model approach, we first evaluate the environmental pressures affecting invertebrate communities in two ecoregions (north, south) using a 23 year time series (1995–2017) and then examine how a set of environmental and physical variables affect the stability of these communities. Results show that lake latitude, size, total phosphorus and alkalinity affect community composition in northern and southern lakes. We find that lake stability is affected by species richness and lake size in both ecoregions and alkalinity and total phosphorus in northern lakes. There is large heterogeneity in the patterns of community stability of individual lakes, but relationships between that stability and environmental drivers begin to emerge when the lakescape, composed of many discrete lakes, is the focal unit of study. The results of this study highlight that broad‐scale comparisons in combination with long time series are essential to understand the effects of environmental change on the stability of lake communities in space and time. 相似文献
242.
Pragya Paramita Pal Sajeli A. Begum Ameer S. Basha Hiroshi Araya Yoshinori Fujimoto 《化学与生物多样性》2023,20(3):e202200840
Inhibiting nitric oxide (NO) or its production is found to be of therapeutic benefit. To discover natural small molecule inhibitors of NO production, a bioassay- and LC/MS-guided chemical investigation was done on the metabolites of endophytic fungus isolated from fresh Piper nigrum fruits. The isolated pure strain was identified as Penicillium polonicum by 16S rDNA sequence comparison. The culture broth extract of P. polonicum (EEPP) exhibited a significant reduction of NO production (Griess method) in LPS-stimulated RAW 264.7 cells (P<0.0001). To understand the chemical constituents of bioactive EEPP, column chromatography and p-TLC studies were carried out, which yielded eight pure compounds. They were characterised as botryosphaeridione ( 1 ), 3-(3,5-di-tert-butyl-4-hydroxy)phenylpropionic acid ( 2 ), variabilone ( 3 ), 2,4-di-tert-butylphenol ( 4 ), indole-3-carboxylic acid ( 5 ), tyrosol ( 6 ), ethyl ferulate ( 7 ) and a new lignan ( 8 ) based on the spectral analysis. The structure elucidation of the new lignan, named polonilignan ( 8 ), was based on HR-MS, 1H- & 13C-NMR, H−H COSY, HSQC and HMBC spectra. Compounds 2 , 4 , 5 and 6 showed a significant decrease (P<0.0001) in the production of NO in LPS-induced RAW 264.7 cells. Tyrosol ( 6 ) and indole-3-carboxylic acid ( 5 ) controlled nitrite release with IC50 values of 22.84 and 55.01 μM, respectively. This is the first report of (i) P. polonicum as an endophytic fungus of pepper fruits, (ii) isolation of compounds 1 – 8 except 6 from P. polonicum culture broth extract and (iii) NO inhibition effect of 2 , 4 , 5 and 6 . 相似文献
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G Matsuda T Maita Y Suzuyama M Setoguchi Y Ota A Araya M Goodman J Barnabas W Prychodko 《Hoppe-Seyler's Zeitschrift für physiologische Chemie》1973,354(10-11):1517-1520
246.
P. Figueroa I. Gómez R. Carmona L. Holuigue A. Araya X. Jordana 《Molecular & general genetics : MGG》1999,262(1):139-144
The transfer of genetic information from the mitochondrion to the nucleus is thought to be still underway in higher plants.
The mitochondrial genome of Arabidopsis thaliana contains only one rps14 pseudogene. In this paper we show that the functional gene encoding mitochondrial ribosomal protein S14 has been translocated
to the nucleus. This gene transfer is a recent evolutionary event, which occurred within Cruciferae, probably after the divergence
of Arabidopsis and Brassica napus. A 5′ extension of the rps14 reading frame encodes a presequence which, in vitro, targets the polypeptide to isolated mitochondria and is cleaved off
during or after import. No intron was found at the junction of the targeting presequence with the mitochondrially derived
sequence, which are directly connected. By contrast, a 90-bp intron, which is removed by splicing to give a mature poly(A)+mRNA of 0.9 kb, is located in the 3′ non-coding region. To our knowledge, this is the first report of an intron in such a
position in a functional transferred gene in higher plants, and suggests that exon shuffling may have been involved in the
acquisition of elements necessary for expression in the nucleus. Putative roles of this intron in polyadenylation and enhancement
of gene expression are discussed.
Received: 11 January 1999 / Accepted: 27 April 1999 相似文献
247.
Hiroyasu Katayama Masato Furuhashi Araya Umetsu Fumihito Hikage Megumi Watanabe Hiroshi Ohguro Yosuke Ida 《Current issues in molecular biology》2021,43(3):1715
In the current study, to elucidate the pathological characteristics of myopic scleral stroma, three-dimensional (3D) cultures of human scleral stroma fibroblasts (HSSFs) with several axial lengths (AL, 22.80–30.63 mm) that were obtained from patients (n = 7) were examined. Among the three groups of ALs, <25 mm (n = 2), 25–30 mm (n = 2), and >30 mm (n = 3), the physical properties of the 3D HSSFs spheroids with respect to size and stiffness, the expressions of extracellular matrix (ECM) molecules, including collagen (COL) 1, 4, and 6 and fibronectin (FN) by qPCR and immunohistochemistry (IHC), and the mRNA expression of ECM metabolism modulators including hypoxia-inducible factor 1A (HIF 1A), HIF 2A, lysyl oxidase (LOX), tissue inhibitor of metalloproteinase (TIMP) 1–4, and matrix metalloproteinase (MMP) 2, 9, and 14 as well as several endoplasmic reticulum (ER) stress-related factors were compared. In the largest AL group (>30 mm), the 3D HSSFs spheroids were (1) significantly down-sized and less stiff compared to the other groups, and (2) significant changes were detected in the expression of some ECMs (qPCR; the up-regulation of COL1 and COL4, and the down-regulation of FN, IHC; the up-regulation of COL1 and FN, and down-regulation of COL4). The mRNA expressions of ECM modulators and ER stress-related genes were also altered with increasing AL length (up-regulation of HIF2A, MMP2, XBP1, and MMP14, down-regulation of LOX, TIMP 2 and 3, GRP78, GRP94, IRE1, and ATF6). In addition, a substantial down-regulation of some ER stress-related genes (ATF4, sXPB1 and CHOP) was observed in the 25–30 mm AL group. The findings presented herein suggest that small and stiffer 3D HSSFs spheroids in the largest AL group may accurately replicate the pathological significance of scleral thinning and weakening in myopic eyes. In addition, the modulation of several related factors among the different AL groups may also provide significant insights into our understanding of the molecular mechanisms responsible for causing myopic changes in the sclera. 相似文献
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249.
Juan Francisco Araya 《ZooKeys》2013,(257):89-101
A new species of the genus Aeneator Finlay, 1926 is described from off the coast of Caldera (27°S), northern Chile. Aeneator martae sp. n. has a small, broad, stout, angulated shell with more prominent axial ribs and a more obviously keeled periphery than all previously named Chilean species. Comparisons are provided with all other South American named species of Aeneator. 相似文献
250.
Laboratory observation of siblicide with hatching asynchrony in an insect with parental provisioning
We report laboratory observation of siblicide with hatching asynchrony in the passalid beetle Cylindrocaulus patalis. Parents construct a breeding gallery in rotten logs and show complex care of offspring, including provisioning. Clutch sizes
are extremely small, and only a single offspring is raised in a colony in each breeding season. We introduced field-collected
colonies into an artificial gallery and recorded the behaviour of colony members. Eggs hatched at 2- to 10-day intervals in
each colony. Fatal fighting invariably occurred among the larvae soon after hatching of the junior larva, and a single larva,
usually the senior one, survived. 相似文献