全文获取类型
收费全文 | 670篇 |
免费 | 44篇 |
国内免费 | 9篇 |
出版年
2022年 | 10篇 |
2021年 | 11篇 |
2020年 | 7篇 |
2019年 | 4篇 |
2018年 | 7篇 |
2017年 | 11篇 |
2016年 | 11篇 |
2015年 | 17篇 |
2014年 | 13篇 |
2013年 | 16篇 |
2012年 | 32篇 |
2011年 | 32篇 |
2010年 | 21篇 |
2009年 | 26篇 |
2008年 | 38篇 |
2007年 | 28篇 |
2006年 | 39篇 |
2005年 | 14篇 |
2004年 | 24篇 |
2003年 | 21篇 |
2002年 | 19篇 |
2001年 | 18篇 |
2000年 | 22篇 |
1999年 | 29篇 |
1998年 | 9篇 |
1997年 | 7篇 |
1996年 | 11篇 |
1995年 | 3篇 |
1994年 | 4篇 |
1989年 | 2篇 |
1983年 | 4篇 |
1982年 | 2篇 |
1980年 | 4篇 |
1979年 | 2篇 |
1978年 | 5篇 |
1974年 | 4篇 |
1972年 | 2篇 |
1959年 | 7篇 |
1958年 | 23篇 |
1957年 | 26篇 |
1956年 | 26篇 |
1955年 | 22篇 |
1954年 | 22篇 |
1953年 | 13篇 |
1952年 | 13篇 |
1951年 | 10篇 |
1950年 | 9篇 |
1949年 | 2篇 |
1948年 | 2篇 |
1912年 | 2篇 |
排序方式: 共有723条查询结果,搜索用时 31 毫秒
41.
42.
43.
44.
45.
46.
An enzyme-containing microfluidic biochip has been developed for the oxidative polymerization of phenols. The biochip consists of a simple T-junction with two feed reservoirs 20 mm apart and a microreaction channel 30 mm long. The channel is 15 microm deep and 200 microm wide at the center, giving a reaction volume of 90 nL. The biochip was fabricated using conventional photolithographic methods on a glass substrate etched using a HF-based solution. Fluid transport was enabled using electroosmotic flow. Soybean peroxidase was used as the phenol oxidizing catalyst, and in the presence of p-cresol and H(2)O(2), essentially complete conversion of the H(2)O(2) (the limiting substrate) occurred in the microchannel at a flow rate of ca. 290 nL/min. Thus, peroxidase was found to be intrinsically active even upon dramatic scale-down as achieved in microfluidic reactors. These results were extended to a series of phenols, thereby demonstrating that the microfluidic peroxidase reactor may have application in high-throughput screening of phenolic polymerization reactions for use in phenolic resin synthesis. Finally, rapid growth of poly(p-cresol) on the walls of the microreaction channel could be performed in the presence of higher H(2)O(2) concentrations. This finding suggests that solution-phase peroxidase catalysis can be used in the controlled deposition of polymers on the walls of microreactors. 相似文献
47.
Aravind L 《Trends in biochemical sciences》2001,26(9):524-526
A previously uncharacterized 110-125 residue-long domain was identified both in the physiologically important enzyme dopamine beta-monooxygenase and in several other secreted and transmembrane proteins such as SDR2 and CG-6. This domain was predicted to adopt an all-beta fold with seven or eight strands and might function as a module mediating a range of extracellular adhesive interactions. 相似文献
48.
The DNA-repair protein AlkB, EGL-9, and leprecan define new families of 2-oxoglutarate- and iron-dependent dioxygenases 总被引:1,自引:0,他引:1
Background
Protein fold recognition using sequence profile searches frequently allows prediction of the structure and biochemical mechanisms of proteins with an important biological function but unknown biochemical activity. Here we describe such predictions resulting from an analysis of the 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenases, a class of enzymes that are widespread in eukaryotes and bacteria and catalyze a variety of reactions typically involving the oxidation of an organic substrate using a dioxygen molecule. 相似文献49.
50.
Sharma VM Prasanna P Seshu KV Renuka B Rao CV Kumar GS Narasimhulu CP Babu PA Puranik RC Subramanyam D Venkateswarlu A Rajagopal S Kumar KB Rao CS Mamidi NV Deevi DS Ajaykumar R Rajagopalan R 《Bioorganic & medicinal chemistry letters》2002,12(17):2303-2307
In our endeavor to design and synthesize novel anticancer agents, a new series of indoloquinazoline compounds were prepared and tested initially for anticancer activity in vitro against a panel of human cancer cell lines. Most of these compounds exhibited cytotoxic activity in in vitro screens. Compounds were selected and further evaluated using a modified Hollow Fiber Assay for their preliminary in vivo activity against 12 cell lines implanted in the subcutaneous and intraperitoneal compartments in mice. The results indicate that these compounds may constitute a new class of anticancer agents. 相似文献