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201.
Estavillo JM Merino P Pinto M Yamulki S Gebauer G Sapek A Corré W 《Plant and Soil》2002,239(2):253-265
Soils are an important source of N2O, which can be produced both in the nitrification and the denitrification processes. Grassland soils in particular have a high potential for mineralization and subsequent nitrification and denitrification. When ploughing long term grassland soils, the resulting high supply of mineral N may provide a high potential for N2O losses. In this work, the short-term effect of ploughing a permanent grassland soil on gaseous N production was studied at different soil depths. Fertiliser and irrigation were applied in order to observe the effect of ploughing under a range of conditions. The relative proportions of N2O produced from nitrification and denitrification and the proportion of N2 gas produced from denitrification were determined using the methyl fluoride and acetylene specific inhibitors. Irrespectively to ploughing, fertiliser application increased the rates of N2O production, N2O production from nitrification, N2O production from denitrification and total denitrification (N2O + N2). Application of fertiliser also increased the denitrification N2O/N2 ratio both in the denitrification potential and in the gaseous N productions by denitrification. Ploughing promoted soil organic N mineralization which led to an increase in the rates of N2O production, N2O production from nitrification, N2O production from denitrification and total denitrification (N2O + N2). In both the ploughed and unploughed treatments the 0–10 cm soil layer was the major contributing layer to gaseous N production by all the above processes. However, the contribution of this layer decreased by ploughing, gaseous N productions from the 10 to 30 cm layer being significantly increased with respect to the unploughed treatment. Ploughing promoted both nitrification and denitrification derived N2O production, although a higher proportion of N2O lost by denitrification was observed as WFPS increased. Recently ploughed plots showed lower denitrification derived N2O percentages than those ploughed before as a result of the lower soil water content in the former plots. Similarly, a lower mean nitrification derived N2O percentage was found in the 10–30 cm layer compared with the 0–10 cm. 相似文献
202.
Zirconyl hematoxylin stains acidic mucins darkly and specifically using a solution of 100 mg hematoxylin, 5 ml ethanol, 5 ml 0.5% sodium iodate, 400 mg zirconyl chloride octahydrate, and 30 ml 25% aqueous glycerol. The stain is especially advantageous for studying goblet cells and Paget cells. 相似文献
203.
Davide?Baú Alberto?JM?Martin Catherine?Mooney Alessandro?Vullo Ian?Walsh Gianluca?PollastriEmail author 《BMC bioinformatics》2006,7(1):402
Background
We describe Distill, a suite of servers for the prediction of protein structural features: secondary structure; relative solvent accessibility; contact density; backbone structural motifs; residue contact maps at 6, 8 and 12 Angstrom; coarse protein topology. The servers are based on large-scale ensembles of recursive neural networks and trained on large, up-to-date, non-redundant subsets of the Protein Data Bank. Together with structural feature predictions, Distill includes a server for prediction of C α traces for short proteins (up to 200 amino acids). 相似文献204.
Size homoplasy and mutational processes of interrupted microsatellites in two bee species, Apis mellifera and Bombus terrestris (Apidae) 总被引:6,自引:0,他引:6
Similar microsatellite electromorphs (PCR products of the same size) can
arise from independent mutational events. Such alleles are not identical by
descent. This phenomenon, termed size homoplasy, was studied by sequencing
electromorphs of two microsatellite loci in which the stretch of basic
repeats is interrupted by different short (1-2 bp) DNA motifs. The number
and position of these interruptions were established for electromorphs from
closely and distantly related populations of honeybees and bumblebees. No
sequence difference was found when electromorphs came from the same
subspecies or from closely related subspecies, suggesting that they were
probably identical by descent. In contrast, sequence differences were often
detected in distantly related subspecies, showing that size homoplasy
frequently occurs at this level of population differentiation. Size
homoplasy is increased by limits to free length variation of alleles, a
phenomenon that seems to act on interrupted microsatellites when comparing
distantly related taxa, that is, honeybee subspecies from different
evolutionary lineages. Electromorph sequences suggest that, within the
scope of these limits, large mutation events have occurred frequently at
both interrupted loci studied. In good agreement with the molecular data,
computations based on the observed heterozygosity and number of
electromorphs and simulation studies showed that neither locus fits the
one-step stepwise mutant model (SMM). We speculate that interrupted
microsatellites in general could be characterized by a higher variance in
repeat number and consequently a lower homoplasy rate than pure ones.
Hence, interrupted microsatellites should be most appropriate for
investigating population differentiation and evolutionary relationship
between relatively distant populations.
相似文献
205.
Plant Cyclic Nucleotide Signalling: Facts and Fiction 总被引:1,自引:0,他引:1
206.
207.
Background
Biological data resources have become heterogeneous and derive from multiple sources. This introduces challenges in the management and utilization of this data in software development. Although efforts are underway to create a standard format for the transmission and storage of biological data, this objective has yet to be fully realized. 相似文献208.
Evolution of the secondary structures and compensatory mutations of the ribosomal RNAs of Drosophila melanogaster 总被引:10,自引:0,他引:10
This paper examines the effects of DNA sequence evolution on RNA secondary
structures and compensatory mutations. Models of the secondary structures
of Drosophila melanogaster 18S ribosomal RNA (rRNA) and of the complex
between 2S, 5.8S, and 28S rRNAs have been drawn on the basis of comparative
and energetic criteria. The overall AU richness of the D. melanogaster
rRNAs allows the resolution of some ambiguities in the structures of both
large rRNAs. Comparison of the sequence of expansion segment V2 in D.
melanogaster 18S rRNA with the same region in three other Drosophila
species and the tsetse fly (Glossina morsitans morsitans) allows us to
distinguish between two models for the secondary structure of this region.
The secondary structures of the expansion segments of D. melanogaster 28S
rRNA conform to a general pattern for all eukaryotes, despite having highly
divergent sequences between D. melanogaster and vertebrates. The 70 novel
compensatory mutations identified in the 28S rRNA show a strong (70%) bias
toward A-U base pairs, suggesting that a process of biased mutation and/or
biased fixation of A and T point mutations or AT-rich slippage-generated
motifs has occurred during the evolution of D. melanogaster rDNA. This
process has not occurred throughout the D. melanogaster genome. The
processes by which compensatory pairs of mutations are generated and spread
are discussed, and a model is suggested by which a second mutation is more
likely to occur in a unit with a first mutation as such a unit begins to
spread through the family and concomitantly through the population.
Alternatively, mechanisms of proofreading in stem-loop structures at the
DNA level, or between RNA and DNA, might be involved. The apparent
tolerance of noncompensatory mutations in some stems which are otherwise
strongly supported by comparative criteria within D. melanogaster 28S rRNA
must be borne in mind when compensatory mutations are used as a criterion
in secondary-structure modeling. Noncompensatory mutation may extend to the
production of unstable structures where a stem is stabilized by RNA-
protein or additional RNA-RNA interactions in the mature ribosome. Of
motifs suggested to be involved in rRNA processing, one (CGAAAG) is
strongly overrepresented in the 28S rRNA sequence. The data are discussed
both in the context of the forces involved with the evolution of multigene
families and in the context of molecular coevolution in the rDNA family in
particular.
相似文献
209.
Díaz López E A Morales Ruíz EJ Vargas Ramírez P Hernández Herrera RC Reséndiz Melgar I Brena Hernández JM Loeza Corte 《Phyton》2015,84(2):319-330
The effect of six phosphorus levels (0, 40, 80, 120, 160 and 200 kg/ha) on the duration of cumulative leaf area, biomass and agronomic yield was determined in the maize cultivars: Amarillo Almoloya, Cacahuacintle and Condor in 2010 and 2011. Such cultivars were sown in the Cerrillo Piedras Blancas Mexico. A completely randomized complete block design with factorial arrangement was utilized. High phosphorus levels (120, 160 and 200 kg/ha) positively affected the duration of cumulative leaf area; greatest values were obtained in Cacahuacintle. A greater duration of accumulated leaf area contributes to determine high values of biomass accumulation and grain yield in this cultivar. Leaf area duration appeared to be a useful tool for evaluating different genotypes in a given environment. 相似文献
210.