全文获取类型
收费全文 | 172篇 |
免费 | 5篇 |
出版年
2023年 | 3篇 |
2022年 | 1篇 |
2021年 | 5篇 |
2020年 | 2篇 |
2019年 | 2篇 |
2018年 | 2篇 |
2017年 | 4篇 |
2016年 | 6篇 |
2015年 | 7篇 |
2014年 | 8篇 |
2013年 | 5篇 |
2012年 | 22篇 |
2011年 | 10篇 |
2010年 | 3篇 |
2009年 | 2篇 |
2008年 | 8篇 |
2007年 | 13篇 |
2006年 | 10篇 |
2005年 | 7篇 |
2003年 | 5篇 |
2002年 | 5篇 |
2001年 | 1篇 |
2000年 | 1篇 |
1997年 | 1篇 |
1996年 | 3篇 |
1995年 | 2篇 |
1992年 | 1篇 |
1991年 | 4篇 |
1990年 | 3篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1983年 | 2篇 |
1982年 | 3篇 |
1981年 | 3篇 |
1980年 | 2篇 |
1978年 | 1篇 |
1977年 | 3篇 |
1976年 | 1篇 |
1975年 | 3篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1972年 | 1篇 |
1970年 | 1篇 |
1966年 | 1篇 |
1961年 | 1篇 |
排序方式: 共有177条查询结果,搜索用时 20 毫秒
81.
Arati Das 《Mycopathologia》1973,49(2-3):205-207
82.
83.
A strain of Aspergillus terreus 4 was found to show extracellular amylolytic activity and the amylase was identified as glucoamylase enzyme. The optimum temperature for the enzyme activity was 60% and it was stable at this temperature for 1 h. The enzyme was optimally active at pH 5.0 and stable between pH 3.0-8.0. Km values of glucoamylase for soluble starch, amylose and amylopectin were 5.9 mg/ml, 4.8 mg/ml and 2.6 mg/ml respectively. 相似文献
84.
85.
86.
87.
Ruppert SM Li W Zhang G Carlson AL Limaye A Durum SK Khaled AR 《Biochimica et biophysica acta》2012,1823(10):1877-1893
Bim is a BH3-only member of the Bcl-2 family that enables the death of T-cells. Partial rescue of cytokine-deprived T-cells occurs when Bim and the receptor for the T-cell growth factor, interleukin-7, are deleted, implicating Bim as a possible target of interleukin-7-mediated signaling. Alternative splicing yields three major isoforms: BimEL, BimL and BimS. To study the effect of Bim deficiency and define the function of the major isoforms, Bim-containing and Bim-deficient T-cells, dependent on interleukin-7 for growth, were used. Loss of total Bim in interleukin-7-deprived T-cells resulted in delayed apoptosis. However, loss of Bim also impeded the later degradative phase of autophagy. p62, an autophagy-adaptor protein which is normally degraded, accumulated in Bim deficient cells. To explain this, BimL was found to support acidification of lysosomes that later may associate with autophagic vesicles. Key findings showed that inhibition of lysosomal acidification accelerated death upon interleukin-7 withdrawal only in Bim-containing T-cells. intereukin-7 dependent T-cells lacking Bim were less sensitive to inhibition of lysosomal acidification. BimL co-immunoprecipitated with dynein and Lamp1-containing vesicles, indicating BimL could be an adaptor for dynein to facilitate loading of lysosomes. In Bim deficient T-cells, lysosome-tracking probes revealed vesicles of less acidic pH. Over-expression of BimL restored acidic vesicles in Bim deficient T-cells, while other isoforms, BimEL and BimS, promoted intrinsic cell death. These results reveal a novel role for BimL in lysosomal positioning that may be required for the formation of degradative autolysosomes. 相似文献
88.
Avdhoot D. Velankar Gianluca Quintini Arati Prabhu Alexander Weber Gundula Hunaeus Britta Voland Monika Wuest Christian Orjeda Dipak Harel Shaji Varghese Vikas Gore Meenal Patil Deepak Gayke Matthias Herdemann Isabelle Heit Andrea Zaliani 《Bioorganic & medicinal chemistry》2010,18(12):4547-4559
Interleukin-2 inducible T-cell kinase (ITK) is one of five kinases that belong to the Tec kinase family that plays an important role in T-cell and mast cell signaling. Various reports point to a role of ITK in the treatment of allergic asthma. For example, it was shown that mice lacking ITK have reduced airway hyperresponsiveness, inflammation and tracheal responses in an allergic asthma model. In this article, we disclose novel ITK inhibitors based on (4 or 5-aryl)pyrazolyl-indole scaffold that were also found to be selective for ITK over other kinases like IRK, CDK2, GSK3ß and PKA. 相似文献
89.
Vasiyani Hitesh Shinde Anjali Roy Milton Mane Minal Singh Kritarth Singh Jyoti Gohel Dhruv Currim Fatema Vaidya Khushali Chhabria Mahesh Singh Rajesh 《Apoptosis : an international journal on programmed cell death》2021,26(5-6):293-306
Apoptosis - Immune adaptor protein like STING/MITA regulate innate immune response and plays a critical role in inflammation in the tumor microenvironment and regulation of metastasis including... 相似文献
90.
SubbaRao V. Madhunapantula Arati Sharma Raghavendra Gowda Gavin P. Robertson 《Pigment cell & melanoma research》2013,26(6):886-899
Deregulated expression or activity of kinases can lead to melanomas, but often the particular kinase isoform causing the effect is not well established, making identification and validation of different isoforms regulating disease development especially important. To accomplish this objective, an siRNA screen was undertaken that which identified glycogen synthase kinase 3α (GSK3α) as an important melanoma growth regulator. Melanocytes and melanoma cell lines representing various stages of melanoma tumor progression expressed both GSK3α and GSK3β, but analysis of tumors in patients with melanoma showed elevated expression of GSK3α in 72% of samples, which was not observed for GSK3β. Furthermore, 80% of tumors in patients with melanoma expressed elevated levels of catalytically active phosphorylated GSK3α (pGSK3αY279), but not phosphorylated GSK3β (pGSK3βY216). siRNA‐mediated reduction in GSK3α protein levels reduced melanoma cell survival and proliferation, sensitized cells to apoptosis‐inducing agents and decreased xenografted tumor development by up to 56%. Mechanistically, inhibiting GSK3α expression using siRNA or the pharmacological agent AR‐A014418 arrested melanoma cells in the G0/G1 phase of the cell cycle and induced apoptotic death to retard tumorigenesis. Therefore, GSK3α is a key therapeutic target in melanoma. 相似文献