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Sharma  Jeetesh  Prabha  Punit  Sharma  Rohit  Gupta  Shalini  Dixit  Aparna 《Cytotechnology》2022,74(5):591-611
Cytotechnology - Myeloid leukemia is one of the major causes of deaths among elderly with very poor prognosis. Due to the adverse effects of existing chemotherapeutic agents, plant-derived...  相似文献   
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An endophytic fungus isolated from Camellia sinensis, Assam, Northeastern India was identified as Colletotrichum gloeosporioides on the basis of morphological characteristics and rDNA ITS analysis. This endophytic fungus was evaluated for growth inhibition against tea pathogens Pestalotiopsis theae and Colletotrichum camelliae. One isolate of C. gloeosporioides showed strong antagonistic activity against Pestalotiopsis theae (64 %) and moderate activity against C. camelliae (37 %). Fifty percent cell-free culture filtrate from 5-day-old cultures showed highest antagonistic activity against both the pathogens although the inhibition percent was less as compared to dual culture. In the experiment of volatile compounds none of the isolates of C. gloeosporioides strains showed visible inhibition against P. theae and C. camelliae. The activity of extracellular hydrolytic enzymes chitinase and protease was also high in this culture fluid and measured 10 and 4.3 IU/μl, respectively.  相似文献   
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Development of a broad-spectrum fluorescent heavy metal bacterial biosensor   总被引:1,自引:0,他引:1  
Bacterial biosensors can measure pollution in terms of their actual toxicity to living organisms. A recombinant bacterial biosensor has been constructed that is known to respond to toxic levels of Zn2+, Cd2+ and Hg2+. The zinc regulatory gene zntR and zntA promoter from znt operon of E. coli have been used to trigger the expression of GFP reporter protein at toxic levels of these ions. The sensor was induced with 3–800?ppm of Zn2+, 0.005–4?ppm of Cd2+ and 0.001–0.12?ppm of Hg2+ ions. Induction studies were also performed in liquid media to quantify GFP fluorescence using fluorimeter. To determine the optimum culture conditions three different incubation periods (16, 20 and 24?h) were followed. Results showed an increased and consistent fluorescence in cells incubated for 16?h. Maximum induction for Zn2+, Cd2+ and Hg2+ was observed at 20, 0.005 and 0.002?ppm, respectively. The pPROBE-zntR-zntA biosensor reported here can be employed as a primary screening technique for aquatic heavy metal pollution.  相似文献   
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Bacillus licheniformis solubilized a range of inorganic phosphates and five different Indian rock phosphates (low grade) to varying extent in broth culture and in soil. Statistical evaluation indicated that broth cultures alone were inadequate indicators of the potential for microbial solubilization of rock phosphates.  相似文献   
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