全文获取类型
收费全文 | 464篇 |
免费 | 38篇 |
专业分类
502篇 |
出版年
2023年 | 3篇 |
2022年 | 2篇 |
2021年 | 11篇 |
2020年 | 8篇 |
2019年 | 9篇 |
2018年 | 5篇 |
2017年 | 8篇 |
2016年 | 11篇 |
2015年 | 19篇 |
2014年 | 24篇 |
2013年 | 16篇 |
2012年 | 33篇 |
2011年 | 23篇 |
2010年 | 17篇 |
2009年 | 15篇 |
2008年 | 30篇 |
2007年 | 19篇 |
2006年 | 16篇 |
2005年 | 16篇 |
2004年 | 22篇 |
2003年 | 10篇 |
2002年 | 11篇 |
2001年 | 17篇 |
2000年 | 14篇 |
1999年 | 15篇 |
1998年 | 12篇 |
1997年 | 4篇 |
1996年 | 5篇 |
1995年 | 8篇 |
1994年 | 5篇 |
1993年 | 7篇 |
1992年 | 8篇 |
1991年 | 6篇 |
1990年 | 5篇 |
1989年 | 6篇 |
1988年 | 5篇 |
1987年 | 3篇 |
1986年 | 4篇 |
1985年 | 4篇 |
1984年 | 9篇 |
1983年 | 5篇 |
1982年 | 5篇 |
1981年 | 5篇 |
1975年 | 3篇 |
1973年 | 1篇 |
1972年 | 1篇 |
1971年 | 3篇 |
1970年 | 3篇 |
1969年 | 4篇 |
1968年 | 2篇 |
排序方式: 共有502条查询结果,搜索用时 0 毫秒
41.
L. Gómez‐Aparicio I. M. Pérez‐Ramos I. Mendoza L. Matías J. L. Quero J. Castro R. Zamora T. Marañón 《Oikos》2008,117(11):1683-1699
Understanding seedling performance across resource gradients is crucial for defining the regeneration niche of plant species under current environmental conditions and for predicting potential changes under a global change scenario. A 2‐year field experiment was conducted to determine how seedling survival and growth of two evergreen and two deciduous Quercus species vary along gradients of light and soil properties in two Mediterranean forests with contrasting soils and climatic conditions. Half the seedlings were subjected to an irrigation treatment during the first year to quantify the effects on performance of an alteration in the summer drought intensity. Linear and non‐linear models were parameterized and compared to identify major resources controlling seedling performance. We found both site‐specific and general patterns of regeneration. Strong site‐specificity was found in the identity of the best predictors of seedling survival: survival decreased linearly with increasing light (i.e. increasing desiccation risk) in the drier site, whereas it decreased logistically with increasing spring soil water content (i.e. increasing waterlogging risk) in the wetter site. We found strong empirical support for multiple resource limitation at the drier site, the response to light being modulated by the availability of soil resources (water and P). Evidence for regeneration niche partitioning among Quercus species was only found at the wetter site. However, at both sites Quercus species shared the same response to summer drought alleviation through water addition: increased first‐year survival but not final survival (i.e. after two years). This suggests that extremely dry summers (i.e. the second summer in the experiment) can cancel out the positive effects of previous wetter summers. Therefore, an increase in the intensity and frequency of summer drought with climate change might cause a double negative impact on Quercus regeneration, due to a general reduction in survival probability and the annulment of the positive effects of (infrequent) ‘wet’ years. Overall, results presented in this study are a major step towards the development of a mechanistic model of Mediterranean forest dynamics that incorporates the idiosyncrasies and generalities of tree regeneration in these systems, and that allow simulation and prediction of the ecological consequences of resource level alterations due to global change. 相似文献
42.
Aparicio Enric Rocaspana Rafel Palau-Ibars Antoni de Sostoa Adolfo Alcaraz Carles 《Ichthyological Research》2023,70(1):101-109
Ichthyological Research - The Mediterranean brown trout (Salmo trutta complex) is native to basins draining to the Western Mediterranean Sea, and natural populations are currently declining in... 相似文献
43.
Milagros Bürgi Gabriela Inés Aparicio Aquiles Dorella Ricardo Kratje Camila Scorticati Marcos Oggero 《Biotechnology journal》2021,16(5):2000455
Neurological disorders affect millions of people causing behavior-cognitive disabilities. Nowadays they have no effective treatment. Human erythropoietin (hEPO) has been clinically used because of its neurotrophic and cytoprotective properties. However, the erythropoietic activity (EA) should be considered as a side effect. Some analogs like non-sialylated EPO, carbamylated EPO, or EPO peptides have been developed showing different weaknesses: erythropoiesis preservation, low stability, potential immunogenicity, or fast clearance. Herein, we used a novel strategy that blocks the EA but preserves hEPO neurobiological actions. N-glycoengineering was accomplished to add a new glycosylation site within the hEPO sequence responsible for its EA. hEPO-derivatives were produced by CHO.K1 cells, affinity-purified and functionally analyzed studying their in vitro and in vivo EA, their in vitro neuronal plasticity in hippocampal neurons and their neuroprotective action by rescuing hippocampal neurons from apoptosis. Muteins Mut 45_47 (K45 > N45 + N47 > T47), Mut 104 (S104 > N104), and Mut 151_153 (G151 > N151 + K153 > T153) lost their EA but preserved their neuroprotection activity and enhanced neuroplasticity more efficiently than hEPO. Interestingly, Mut 45_47 resulted in a promising candidate to explore as neurotherapeutic considering not only its biopotency but also its pharmacokinetic potential due to the hyperglycosylation. 相似文献
44.
Bellón T Heredia AB Llano M Minguela A Rodriguez A López-Botet M Aparicio P 《Journal of immunology (Baltimore, Md. : 1950)》1999,162(7):3996-4002
Some T lymphocytes express the CD94 Ag, which is known to form heterodimers with members of the NKG2 family. We have studied the expression pattern and function of CD94 heterodimers in different alphabeta or gammadelta T cell clones. Most of the CD94+NKG2A- T cells have a low to intermediate expression of CD94 Ag. The cross-linking of the CD94/NKG2 heterodimer in one of these CD8 alphabeta CD94+NKG2A- T cell clones (K14B06) was able to: 1) increase the intracellular concentration of Ca2+, 2) induce the up-regulation of CD25 Ag expression and the secretion of IFN-gamma, and 3) trigger redirected cytotoxicity in a TCR-independent manner. This activatory property was not shared by any other costimulatory molecule expressed by the K14B06 T cell clone, including CD8, CD28, CD45, CD69, or CD2 Ags. The immunoprecipitation of CD94 heterodimer showed a 39-kDa band with a similar m.w. to the activatory heterodimer found on some NK clones. A novel form of the NKG2 family (NKG2H) was identified in K14B06. NKG2H protein represents an alternative spliced form of the NKG2E gene, displaying a charged residue in the transmembrane portion and a cytoplasmic tail that lacks immunoreceptor tyrosine-based inhibitory motifs. The expression of NKG2H in the cell membrane through its association to CD94 and DAP-12 molecules supports that it could form part of the activatory CD94/Kp39 heterodimer present on K14B06 cells. 相似文献
45.
The aim of this work is the characterization of the glycoconjugates of the spermatids during the spermiogenesis of the testis of an urodele amphibian, Pleurodeles waltl, by means of lectins in combination with several chemical and enzymatic procedures, in order to establish the distribution of N- and O-linked oligosaccharides in these cells. The acrosome was the most relevant lectin-labeled structure. The O-linked oligosaccharides contained DBA- and SBA-positive GalNAc, AAA-positive Fuc and PNA-positive Gal1,3GalNAc. Sialic acid was scarcely observed, the Neu5Ac2,-3Gal1,4GlcNAc sequence was found in N-linked oligosaccharides. Additionally, N-linked oligosaccharides containing HPA-positive GalNAc and AAA-positive Fuc were found. Moreover, with some lectins the acrosome showed a variable composition of the oligosaccharides in the different steps of the sperm maturation. Some residues were found only in the early steps in maturating acrosome, while others were in the later steps, showing that acrosomal glycoconjugates are modified during acrosome development in spermiogenesis. The changes observed during acrosome maturation suggest the existence of a predetermined pattern of storage of the acrosome components and a progressive compression of them. 相似文献
46.
Cuadrado Y Fernández M Recio E Aparicio JF Martín JF 《Applied microbiology and biotechnology》2004,64(2):228-236
The first two genes of the threonine pathway, ask and asd, were cloned and sequenced from the aminoethoxyvinylglycine-producing Streptomyces sp. NRRL 5331. The two genes are organized in a bicistronic operon. ask, encoding the apartokinase (ASK), is located upstream from asd. The presence of a ribosome-binding site within the ask sequence suggests that this open reading frame encodes two overlapping proteins. The formation of both subunits of the aspartokinase from a single gene was studied using antibodies raised against the C-terminal end of the aspartokinase subunits. Disruption of asd results in a significant decrease of aminoethoxyvinylglycine production, thus supporting the involvement of the ask–asd operon in the biosynthesis of this metabolite. This is the first report in which a gene cluster for the first two steps of aminoethoxyvinylglycine biosynthesis is characterized.Abbreviations
ASD
Aspartate semialdehyde dehydrogenase
-
ASK
Aspartokinase
-
AVG
Aminoethoxyvinylglycine 相似文献
47.
The evolution of secondary sexual characters is the subject of controversial debate between those defending their role as
‘viability indicators’ and those arguing that ornaments are purely ‘attractive traits’ selected by females. Recent theoretical
studies suggest that these hypotheses are not mutually exclusive, as both viability and attractiveness can contribute to improve
the reproductive success of progeny and could thus simultaneously underlie female choices. If that is the case, strategies
of cheaper advertisement, allowing the expression of larger ornaments for the same cost, could proliferate even in species
in which honest signalling of viability prevails. Under this scenario, different males could invest a different amount of
resources per ornament unit of expression, thus using different signalling rules. We studied the relationship between tail
feather length (a trait that is the subject of a female mate preference) and feather mass (a measure of investment in feather
production) in a barn swallow Hirundo rustica population. Different males used different and consistent signalling rules when developing ornamental feathers. That is,
to produce a feather of a given length, each male used a constant amount of resources across different years, but this amount
varied between males. Although the amount of material invested in feathers (feather mass) is a condition-dependent trait,
the organization of this material in ornamental feathers (i.e. the signalling rules) was not. Neither survival nor risk of
feather breakage was related to the signalling rules. Thus, these results suggest that both ‘viability’ and ‘runaway’ mechanisms
are independent determinants of the evolution of ornamental sexual feathers in the barn swallow. A preference for long tails
will ensure that females either obtain a sire with high viability, or one transferring the capability to produce longer and
more attractive tails at a lower cost of production to its offspring. 相似文献
48.
A Plonquet S Bastuji-Garin F Tahmasebi C Brisacier K Ledudal JP Farcet E Paillaud 《Immunity & ageing : I & A》2011,8(1):1-7
Background
Nosocomial infections are extremely common in the elderly and may be related to ageing of the immune system. The Immune Risk Phenotype (IRP), which predicts shorter survival in elderly patients, has not been evaluated as a possible risk factor for nosocomial infection. Our aim was to assess the prevalence of nosocomial infections in elderly in-patients and to investigate potential relationships between nosocomial infections and the immunophenotype, including IRP parameters.Results
We included 252 consecutive in-patients aged 70 years or over (mean age, 85 ± 6.2 years), between 2006 and 2008. Among them, 97 experienced nosocomial infections, yielding a prevalence rate of 38.5% (95% confidence interval, 32.5-44.5). The main infection sites were the respiratory tract (21%) and urinary tract (17.1%) When we compared immunological parameters including cell counts determined by flow cytometry in the groups with and without nosocomial infections, we found that the group with nosocomial infections had significantly lower values for the CD4/CD8 ratio and naive CD8 and CD4 T-cell counts and higher counts of memory CD8 T-cells with a significant increase in CD28-negative CD8-T cells. Neither cytomegalovirus status (positive in 193/246 patients) nor presence of the IRP was associated with nosocomial infections. However, nosocomial pneumonia was significantly more common among IRP-positive patients than IRP-negative patients (17/60 versus 28/180; p = 0.036).Conclusion
Immunological parameters that are easy to determine in everyday practice and known to be associated with immune system ageing and shorter survival in the elderly are also associated with an elevated risk of nosocomial pneumonia in the relatively short term. 相似文献49.
Effects of pulsed discharges from a hydropower station on summer diel feeding activity and diet of brown trout (Salmo trutta Linnaeus, 1758) in an Iberian stream 下载免费PDF全文
R. Rocaspana E. Aparicio D. Vinyoles A. Palau 《Zeitschrift fur angewandte Ichthyologie》2016,32(1):190-197
The influence of pulsed discharges associated with hydroelectric power generation (i.e. hydropeaking) on feeding activity and diet composition of adult brown trout (Salmo trutta) was studied during the summer by comparing two sites: upstream (control site) and downstream from a power plant (hydropeaking site). Twenty fish were captured from each study site by electrofishing at 4‐hour intervals for two consecutive days and stomach contents were collected with pulsed gastric lavage. Hydropeaking events affected brown trout feeding behaviour as well as prey availability. Feeding intensity, measured by the stomach Fullness Index, showed pronounced variations with maximum values after flow pulses, which were linked to variations in prey availability because of increased drift rates of invertebrates. In contrast, brown trout living at the control site showed smoother variations in feeding activity not linked to invertebrate drift. Overall, brown trout at the hydropeaking site had higher food consumption rates and a more generalist and heterogeneous diet than trout from the control site, indicating an opportunistic feeding behaviour during flow pulses. Therefore, the hydrological disturbance caused by hydropeaking did not appear to cause direct negative impacts on feeding of adult brown trout. However, reduced trout density and imbalanced size structure in the hydropeaking site were detected, requiring further research to clarify the spatial influence of hydropeaking on other factors that could negatively affect brown trout populations. 相似文献
50.