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51.
Edziri Hayet Mastouri Maha Ammar Samia Matieu Mata Patrich Gros Hiar Raida Mahjoub Mohamed Ali Ali Si Mohamed Laurent Gutmann Zine Mighri Aouni Mahjoub 《World journal of microbiology & biotechnology》2008,24(12):2933-2940
Antimicrobial, antioxidant, and antiviral activities of flower extracts of Retama raetam Forssk. Webb (Fabaceae) were screened both from standard and isolated Gram-positive and Gram-negative bacteria by solid medium
technique. Oxacillin, Amoxicillin, Ticarcillin, Cefotaxim, and Amphotericin were used as the control agents. The antiviral
activity was evaluated against human cytomegalovirus (HCMV) strain AD-169 (ATCC Ref. VR 538) and coxsackie B virus type 3
(CoxB-3) using a cytopathic effect (CPE) reduction assay. The antioxidant activity was evaluated using two tests: 1,1-diphenyl-2-picrylhydrazyl
(DPPH) free-radical scavenging and the ammonium thiocyanate methods. All extracts were characterized quantitatively for the
presence of polyphenols, flavonoids, and tannins. Of the extracts tested, butanol and ethyl acetate extracts showed important
antibacterial activity against Gram-positive and Gram-negative bacteria but only moderate antifungal activity. Methanol extract
exhibited moderate antiviral activity against HCMV with IC50 of 250 μg/ml. Ethyl acetate, chloroform, and methanol fractions were found to cause significant free-radical-scavenging effects
in both assays. These results may suggest that R. raetam flowers could be used as a natural preservative ingredient in the food and/or pharmaceutical industries. 相似文献
52.
Amira Bidani Oumèma Nouri-Ellouz Lilia Lakhoua Darasinh Sihachakr Catherine Cheniclet Ali Mahjoub Noureddine Drira Radhia Gargouri-Bouzid 《Plant Cell, Tissue and Organ Culture》2007,91(3):179-189
In this study three somatic hybrid lines originating from protoplast fusion between Solanum tuberosum cv. BF15 and Solanum berthaultii were subjected to a detailed molecular analysis using the I-SSR-PCR technique based on 5′-anchored microsatellite primers.
The data obtained revealed a polymorphism between the different lines, suggesting that they correspond to symmetric hybrids.
The analysis of chloroplast genome of these hybrids showed that they are resulting from a recombination between parental plastomes.
When transferred to a greenhouse, these hybrid lines displayed an improved vigour compared to the cultivated potato BF15 parent.
Indeed, an important growth rate and high tuber yield and weight were obtained for these hybrids compared to the parent. Some
of these hybrids showed also an improved ion homeostasis control and they seem to display a better tolerance to salt stress
compared to the potato BF15 parent. 相似文献
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The Sabin3 mutation in the viral RNA plays an important role in directing attenuation phenotype of Sabin vaccine strain of
poliovirus type 1 (PV1). We previously described that Sabin3-like mutation introduced in Coxsackievirus B3 (CVB3) genome led
to a defective mutant. However, this mutation do not led to destruction of secondary structure motif C within the stem-loop
V of CVB3 RNA because of the presence of one nucleotide difference (C → U) in the region encompassing the Sabin3 mutation
at nucleotides 471 of PV1 and 475 of CVB3 RNA. In order to reproduce the same sequence of PV1 sabin3 vaccine strain, we introduce
in this study an additional mutation (U475 → C) to CVB3 Sabin3-like mutant. Our results demonstrated that Sabin3-like+C mutant displayed a decreased translation initiation
defects when translated in cell-free system. This translation initiation defect was correlated with reduced yields of infectious
virus particles in HeLa cells in comparison with Sabin3-like mutant and wild-type CVB3 viruses. Inoculation of Swiss mice
with mutant viruses resulted in no inflammatory heart disease when compared to heart of mice infected with wild-type. Theses
findings indicate that the double mutant could be exploited for the development of a live attenuated vaccine against CVB3. 相似文献
55.
M. Hamdi S. Hamza N. Mtimet N. Hmida C. Cornelius S. Zgouli A. Mahjoub Ph. Thonart 《Bioprocess and biosystems engineering》2000,22(1):23-27
Summary Three Lactococcus strains (Lactococcus ssp. lactis var. diacetylactis, Lactococcus ssp. lactis cremoris and Lactococcus ssp. lactis var. lactis) isolated from the Tunisian lben were grown at constant pH on CSL medium in stirred fermentors for lactic starters production. The agitation required to homogenate alkali used to pH control should be low because it affects the Lactococcus growth. Scale up from 20-liter fermentor to 400-liter fermentor was carried out at constant impeller tip speed below 150 cm sу. The CSL supplementation and fed-batch with glucose increased the yield in the upper 1010 cfu/ml. The consumed glucose during fermentation was converted into lactic acid and cell. Before fed-batch, the maximum specific growth rate of Lactococcus ssp. lactis var. diacetylactis was around 1 hу and the number of cells increased 20 to 40 times according to inoculum size. After fed-batch, the glucose consumption rate remains constant but specific growth rate decreased and number of cell trebled only. 相似文献
56.
A NIMA-related kinase, Fa2p, localizes to a novel site in the proximal cilia of Chlamydomonas and mouse kidney cells
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Polycystic kidney disease and related syndromes involve dysregulation of cell proliferation in conjunction with ciliary defects. The relationship between cilia and cell cycle is enigmatic, but it may involve regulation by the NIMA-family of kinases (Neks). We previously showed that the Nek Fa2p is important for ciliary function and cell cycle in Chlamydomonas. We now show that Fa2p localizes to an important regulatory site at the proximal end of cilia in both Chlamydomonas and a mouse kidney cell line. Fa2p also is associated with the proximal end of centrioles. Its localization is dynamic during the cell cycle, following a similar pattern in both cell types. The cell cycle function of Fa2p is kinase independent, whereas its ciliary function is kinase dependent. Mice with mutations in Nek1 or Nek8 have cystic kidneys; therefore, our discovery that a member of this phylogenetic group of Nek proteins is localized to the same sites in Chlamydomonas and kidney epithelial cells suggests that Neks play conserved roles in the coordination of cilia and cell cycle progression. 相似文献
57.
Malek Besbes Amel Omri Imed Cheraif Mejda Daami Hichem Ben Jannet Maha Mastouri Mahjoub Aouni Boulbaba Selmi 《化学与生物多样性》2012,9(4):829-839
The essential oils isolated from three organs, i.e., fruits, stems and leaves, and flowers, of the endemic North African plant Scabiosa arenaria Forssk . were screened for their chemical composition, as well as their possible antibacterial, anticandidal, and antifungal properties. According to the GC‐FID and GC/MS analyses, 61 (99.26% of the total oil composition), 79 (98.43%), and 51 compounds (99.9%) were identified in the three oils, respectively. While α‐thujone (34.39%), camphor (17.48%), and β‐thujone (15.29%) constituted the major compounds of the fruit oil, chrysanthenone (23.43%), together with camphor (12.98%) and α‐thujone (10.7%), were the main constituents of the stem and leaf oil. In the case of the flower oil, also chrysanthenone (38.52%), camphor (11.75%), and α‐thujone (9.5%) were identified as the major compounds. Furthermore, the isolated oils were tested against 16 Gram‐positive and Gram‐negative bacteria, four Candida species, and nine phytopathogenic fungal strains. It was found that the oils exhibited interesting antibacterial and anticandidal activities, comparable to those of thymol, which was used as positive control, but no activity against the phytopathogenic fungal strains was observed. 相似文献
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59.
Sdiri-Loulizi K Ambert-Balay K Gharbi-Khelifi H Hassine M Chouchane S Sakly N Neji-Guédiche M Pothier P Aouni M 《Canadian journal of microbiology》2011,57(10):810-819
Rotaviruses are the most common cause of severe viral gastroenteritis in early childhood worldwide. Thus, the objectives of our study were to determine the molecular epidemiology and the clinical features of rotavirus gastroenteritis in Tunisia. Between January 2003 and April 2007, a prospective study was conducted on 788 stool samples collected from children under 12 years of age who were suffering from acute gastroenteritis. Rotavirus was detected by multiplex RT-PCR in 27% (n = 213) of samples, among them 79.3% (n = 169) cases were monoinfections. The frequency of rotavirus infections was significantly higher among inpatients (29%) than among outpatients (13%) (P < 0.001). The seasonal distribution of rotavirus diarrhea showed a winter peak, with an unusual peak from June to September. The mean duration of hospitalization was 6.5 ± 8.1 days and the mean age was 15.8 ± 22.8 months for rotavirus monoinfections. Fever, vomiting, abdominal pain, and dehydration were observed in 88, 98, 13, and 80 cases, respectively, in children with rotavirus monoinfections. G3P[8] (45.6%) and G1P[8] (23.9%) were the most common genotypes found in our study. The determination of rotavirus infection prevalence and the characterization of the rotavirus strains circulating will help us to better understand the molecular biology and epidemiology of the disease in our country. 相似文献
60.
Khodadadi Emad Mahjoub Soleiman Arabi Mehdi Sheikh Najafzadehvarzi Hossein Nasirian Vahid 《Molecular biology reports》2021,48(3):2105-2116
Molecular Biology Reports - Targeted drug delivery vehicles make it possible to deliver anti-cancer drugs to the cells or tissues of interest. Aptamers are peptide or oligonucleotide molecules that... 相似文献