Effect of certain inhibitors of nucleic acid and protein synthesis on the growth and development of amine oxidase and ascorbate oxidase in germinating peas

5-Fluorodeoxyuridine(FUDR), actinomycin D(Act-D), puromycinaminonucleoside( puromycin) and 5-fluorouracil(5-FU) were usedto analyze the development of amine oxidase and ascorbate oxidasein, and the growth of pea seedlings. Both ascorbate oxidasedevelopment and growth were inhibited by administration of FUDR,and this effect was alleviated by the addition of thymidine.The development of amine oxidase was less sensitive to FUDR.Act-D and puromycin significantly inhibited both developmentof the enzyme and growth of the seedlings. On the other hand,5-FU inhibited growth of the seedlings and ascorbate oxiadsedevelopment, but had no remarkable effect on the synthesis ofamine oxidase. (Received September 30, 1974; )     

Non-enzymatic oligomerization of racemic adenosine 5'-phosphorimidazolide on Na(+)-montmorillonite.

In this study, we have investigated non-enzymatic oligomerization of an activated racemic mononucleotide in the presence of Na(+)-montmorillonite. Oligomers up to the decamer in length were formed by oligomerization reactions of activated D- and L-mononucleotides. Similarly, oligomerization of an activated racemic mononucleotide results in the formation of oligomers up to the octamer. These results suggest that montmorillonite catalysis is quite efficient for the oligomerization of racemic monomers, though it is somewhat less efficient than that of D- and L-monomers.