全文获取类型
收费全文 | 741篇 |
免费 | 50篇 |
出版年
2023年 | 1篇 |
2022年 | 9篇 |
2021年 | 15篇 |
2020年 | 10篇 |
2019年 | 9篇 |
2018年 | 14篇 |
2017年 | 16篇 |
2016年 | 24篇 |
2015年 | 34篇 |
2014年 | 44篇 |
2013年 | 42篇 |
2012年 | 76篇 |
2011年 | 47篇 |
2010年 | 35篇 |
2009年 | 39篇 |
2008年 | 53篇 |
2007年 | 52篇 |
2006年 | 47篇 |
2005年 | 44篇 |
2004年 | 35篇 |
2003年 | 23篇 |
2002年 | 25篇 |
2001年 | 10篇 |
2000年 | 5篇 |
1999年 | 8篇 |
1998年 | 11篇 |
1997年 | 5篇 |
1996年 | 8篇 |
1995年 | 3篇 |
1994年 | 6篇 |
1993年 | 6篇 |
1992年 | 7篇 |
1991年 | 4篇 |
1990年 | 5篇 |
1989年 | 3篇 |
1988年 | 4篇 |
1987年 | 2篇 |
1986年 | 1篇 |
1985年 | 2篇 |
1983年 | 3篇 |
1981年 | 1篇 |
1979年 | 1篇 |
1971年 | 1篇 |
1967年 | 1篇 |
排序方式: 共有791条查询结果,搜索用时 290 毫秒
781.
782.
Sunny Jithin S. Nisha Khairun Natarajan Anuradha Saleena Lilly M. 《Extremophiles : life under extreme conditions》2021,25(4):319-325
Extremophiles - Biocatalysts provide many advantages over the traditional chemically assisted processes prevalent in industries. Consequently, the search for novel enzymes has increased over the... 相似文献
783.
Physical ordering of six YACs from the RN region in pigs 总被引:1,自引:0,他引:1
A. Törnsten J.-T. Jeon L. J. Alexander ‡ L. Andersson & B. P. Chowdhary † 《Animal genetics》1998,29(4):319-321
Six YAC clones representing five microsatellite markers from the RN region were mapped by fluorescent in situ hybridization (FISH) on pig metaphase chromosomes and their relative order was determined by pairwise multicolour FISH. Two of the microsatellites viz., Sw120 and Sw936 flank RN as well as the remaining three microsatellites Sw1683, Sw2083 and Sw1309. The results assigned the RN locus to the distal part of the 15q25 band. The linear order of the microsatellites was compared with the available linkage mapping data. 相似文献
784.
785.
K. Byju V. Anuradha Emilda Rosmine N. C. Kumar S. M. Nair 《Journal of plant biochemistry and biotechnology.》2013,22(3):304-311
The composition of the lipophilic extract from Hydrilla verticillata, the common aquatic weed collected from Sasthamkotta Lake, the largest freshwater lake in Kerala, south of the west coast of India, was investigated. The lake is a designated wetland of international importance under the Ramsar Convention since 2002. GC-MS study of the unsaponifiable lipophilic extract of H. verticillata confirmed the presence of 3,5,11,15-tetramethyl 1- hexadecen-3-ol (C20H40O) and phytol (C20H40O) as major components, and their structures were elucidated. Phytol and 3,5,11,15-tetramethyl 1- hexadecen3-ol are the two isomers of the diterpeneol (C20H40O) found in the unsaponifible lipophilic extract of H. verticillata and are formed by the hydrolysis of the alcohol moiety of chlorophyll. On quantification, an appreciable concentration of phytol (6.39 g?Kg?1) was estimated. The feasibility to utilize H. verticillata to produce phytol is to be addressed by further studies since H. verticillata is considered as one of the world’s fast widely spread aquatic weeds on account of its numerous mechanisms of vegetative reproductions. 相似文献
786.
787.
Gatika Agrawal Anuradha Ramesh Pargaonkar Aishwarya Jennifer Sally Maddaly Ravi 《Biotechnology progress》2021,37(3):e3126
Cell cultures are indispensable for both basic and applied research. Advancements in cell culture and analysis increase their utility for basic research and translational applications. A marked development in this direction is advent of three-dimensional (3D) cultures. The extent of advancement in 3D cell culture methods over the past decade has warranted referring to a single cell type being cultured as an aggregate or spheroid using simple scaffolds as “traditional.” In recent years, the development of “next-generation” devices has enabled cultured cells to mimic their natural environments much better than the traditional 3D culture systems. Automated platforms like chip-based devices, magnetic- and acoustics-based assembly devices, di-electrophoresis (DEP), micro pocket cultures (MPoC), and 3D bio-printing provide a dynamic environment compared to the rather static conditions of the traditional simple scaffold-based 3D cultures. Chip-based technologies, which are centered on principles of microfluidics, are revolutionizing the ways in which cell culture and analysis can be compacted into table-top instruments. A parallel evolution in analytical devices enabled efficient assessment of various complex physiological and pathological endpoints. This is augmented by concurrent development of software enabling rapid large-scale automated data acquisition and analysis like image cytometry, elastography, optical coherence tomography, surface-enhanced Raman scattering (SERS), and biosensors. The techniques and devices utilized for the purpose of 3D cell culture and subsequent analysis depend primarily on the requirement of the study. We present here an in-depth account of the devices for obtaining and analyzing 3D cell cultures. 相似文献
788.
Jadhav Uma Mundhe Swapnil Kumar Yashwant Jogaiah Satisha Upadhyay Anuradha Gupta Vidya S. Kadoo Narendra Y. 《Journal of Plant Growth Regulation》2021,40(1):293-304
Journal of Plant Growth Regulation - Compact clusters and small berry size are the major problems associated with the commercialization of table grapes. The application of gibberellic acid 3 (GA3)... 相似文献
789.
V. Thirunavukkarasu A. T. Anitha Nandhini C. V. Anuradha 《Experimental diabetes research》2004,5(4):237-244
Nonenzymatic glycation of proteins, leading to chemical
modification and cross-linking are of importance in the
pathology of diabetic complications.We studied the effect of
α-lipoic acid (LA) on the content and characteristics of the
protein collagen from skin of high-fructose fed rats. The
rats were divided into 4 groups of 6 each. Two groups of
rats were fed with a high fructose diet (60 g/100 g diet) and
administered either LA (35 mg/kg b.w., i.p) (FRU+LA) or
0.2 ml vehicle (saline) (FRU) for 45 days. The other 2 groups
were fed with control diet containing starch (60 g/100 g
diet) and administered either saline (CON) or lipoic acid
(CON+LA). The rats were maintained for 45 days and then
sacrificed. Plasma glucose, insulin, fructosamine, protein
glycation, and blood glycated hemoglobin (HbA1C) were
measured. Collagen was isolated from skin and the physicochemical
properties of collagen were studied. Fructose administration
caused accumulation of collagen in skin. Extensive
cross-linking was evidenced by enhanced glycation
and AGE-linked fluorescence. Increased peroxidation and
changes in physicochemical properties such as shrinkage
temperature, aldehyde content, solubililty pattern, susceptibility
to denaturing agents were observed in fructose-fed
rats. SDS gel pattern of collagen from these rats showed
elevated β component of type I collagen. These changes
were alleviated by the simultaneous administration of LA.
Administration of LA to fructose-fed rats had a positive
influence on both quantitative and qualitative properties of collagen. The results suggest a mechanism for the ability
of LA to delay diabetic complications. 相似文献
790.
M Van Poucke A T?rnsten M Mattheeuws A Van Zeveren L J Peelman B P Chowdhary 《Cytogenetics and cell genetics》1999,85(3-4):279-284
Zoo-FISH and somatic cell hybrid panels have earlier demonstrated extended synteny conservation between human chromosome 3 (HSA3) and pig chromosome 13 (SSC13). In the present study, eight human genes viz., ADCY5, CASR, COL7A1, COL8A1, ITIH1, RHO, SIAT1 and XPC, spread along the length of HSA3, were chosen for expanding the comparative map between the two chromosomes. Using human and rat cDNAs, or human- and porcine-specific PCR products as probes, 8 porcine lambda clones were isolated. After subcloning and partial sequence determination, identity of the clones with regards to the specific genes was established. The eight type 1 markers thus obtained were biotin labeled and FISH mapped to pig metaphase spreads. All lambda clones localized to SSC13. In combination with the hitherto published mapping data of coding sequences on SSC13, a preliminary comparative status depicting the relative organization of this chromosome with respect to HSA3 was developed. The comparative map thus obtained bears significance in searching for candidate genes of economically important traits mapped to SSC13. 相似文献