排序方式: 共有233条查询结果,搜索用时 156 毫秒
121.
Etchells J Peter Moore Lucy Jiang Wen Zhi Prescott Helen Capper Richard Saunders Nigel J Bhatt Anuj M Dickinson Hugh G 《BMC plant biology》2012,12(1):1-12
Studies reported unintended pleiotropic effects for a number of pesticidal proteins ectopically expressed in transgenic crops, but the nature and significance of such effects in planta remain poorly understood. Here we assessed the effects of corn cystatin II (CCII), a potent inhibitor of C1A cysteine (Cys) proteases considered for insect and pathogen control, on the leaf proteome and pathogen resistance status of potato lines constitutively expressing this protein. The leaf proteome of lines accumulating CCII at different levels was resolved by 2-dimensional gel electrophoresis and compared with the leaf proteome of a control (parental) line. Out of ca. 700 proteins monitored on 2-D gels, 23 were significantly up- or downregulated in CCII-expressing leaves, including 14 proteins detected de novo or up-regulated by more than five-fold compared to the control. Most up-regulated proteins were abiotic or biotic stress-responsive proteins, including different secretory peroxidases, wound inducible protease inhibitors and pathogenesis-related proteins. Accordingly, infection of leaf tissues by the fungal necrotroph Botryris cinerea was prevented in CCII-expressing plants, despite a null impact of CCII on growth of this pathogen and the absence of extracellular Cys protease targets for the inhibitor. These data point to the onset of pleiotropic effects altering the leaf proteome in transgenic plants expressing recombinant protease inhibitors. They also show the potential of these proteins as ectopic modulators of stress responses in planta, useful to engineer biotic or abiotic stress tolerance in crop plants of economic significance. 相似文献
122.
Communicated by Ramaswamy H. Sarma 相似文献
123.
124.
Langel FD Jain NA Rossman JS Kingeter LM Kashyap AK Schaefer BC 《The Journal of biological chemistry》2008,283(47):32419-32431
Bcl10 and MALT1 are essential mediators of NF-kappaB activation in response to the triggering of a diverse array of transmembrane receptors, including antigen receptors. Additionally, both proteins are translocation targets in MALT lymphoma. Thus, a detailed understanding of the interaction between these mediators is of considerable biological importance. Previous studies have indicated that a 13-amino acid region downstream of the Bcl10 caspase recruitment domain (CARD) is responsible for interacting with the immunoglobulin-like domains of MALT1. We now provide evidence that the death domain of MALT1 and the CARD of Bcl10 also contribute to Bcl10-MALT1 interactions. Although a direct interaction between the MALT1 death domain and Bcl10 cannot be detected via immunoprecipitation, FRET data strongly suggest that the death domain of MALT1 contributes significantly to the association between Bcl10 and MALT1 in T cells in vivo. Furthermore, analysis of point mutants of conserved residues of Bcl10 shows that the Bcl10 CARD is essential for interaction with the MALT1 N terminus. Mutations that disrupt proper folding of the Bcl10 CARD strongly impair Bcl10-MALT1 interactions. Molecular modeling and functional analyses of Bcl10 point mutants suggest that residues Asp(80) and Glu(84) of helix 5 of the Bcl10 CARD directly contact MALT1. Together, these data demonstrate that the association between Bcl10 and MALT1 involves a complex interaction between multiple protein domains. Moreover, the Bcl10-MALT1 interaction is the second reported example of interactions between a CARD and a non-CARD protein region, which suggests that many signaling cascades may utilize CARD interactions with non-CARD domains. 相似文献
125.
Yeast genomics: past,present, and future promise 总被引:1,自引:0,他引:1
126.
Anuj Dalal Kapeesha Nehra Anjli Hooda Devender Singh Sumit Kumar Rajender Singh Malik 《Luminescence》2022,37(8):1309-1320
Solid ternary europium complexes consisting of fluorinated β-diketone (thenoyltrifluoroacetone, TTFA) and heteroaromatic bidentate auxiliary ligands were synthesized. The luminescence features of the complexes were estimated using various spectral measurements and clearly proved that the Eu3+ ion is efficiently sensitized by ligands by an antenna effect. Photoluminescence excitation spectra have shown that Eu(III) complexes are excited effectively in the ultraviolet (UV) region and the corresponding emission spectra consist of characteristic peaks attributed to the 5D0→7FJ transitions of the europium ion with the strongest emission peak at 611 nm (5D0→7F2). From photoluminescence (PL) data, decay time, Judd–Ofelt parameters, transition rates, and quantum efficiency of the complexes were also determined. The Commission Internationale de l'éclairage (CIE) colour coordinates indicated the bright red emission of ternary europium complexes. Correlated colour temperature values indicated the utilization of these complexes in display devices. Judd–Ofelt and photophysical parameters were also estimated theoretically using LUMPAC software. Various frontier molecular orbitals and their respective energy were determined. These red emissive europium complexes could be utilized for fabricating solid-state lighting systems. 相似文献
127.
128.
BHPMF – a hierarchical Bayesian approach to gap‐filling and trait prediction for macroecology and functional biogeography
下载免费PDF全文
![点击此处可从《Global Ecology and Biogeography》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Franziska Schrodt Jens Kattge Hanhuai Shan Farideh Fazayeli Julia Joswig Arindam Banerjee Markus Reichstein Gerhard Bönisch Sandra Díaz John Dickie Andy Gillison Anuj Karpatne Sandra Lavorel Paul Leadley Christian B. Wirth Ian J. Wright S. Joseph Wright Peter B. Reich 《Global Ecology and Biogeography》2015,24(12):1510-1521
129.
Anuj K. Chandel Bruna C. M. Gonçalves Janice L. Strap 《Critical reviews in biotechnology》2015,35(3):281-293
Lignocellulosic biomass (LB) is a promising sugar feedstock for biofuels and other high-value chemical commodities. The recalcitrance of LB, however, impedes carbohydrate accessibility and its conversion into commercially significant products. Two important factors for the overall economization of biofuel production is LB pretreatment to liberate fermentable sugars followed by conversion into ethanol. Sustainable biofuel production must overcome issues such as minimizing water and energy usage, reducing chemical usage and process intensification. Amongst available pretreatment methods, microorganism-mediated pretreatments are the safest, green, and sustainable. Native biodelignifying agents such as Phanerochaete chrysosporium, Pycnoporous cinnabarinus, Ceriporiopsis subvermispora and Cyathus stercoreus can remove lignin, making the remaining substrates amenable for saccharification. The development of a robust, integrated bioprocessing (IBP) approach for economic ethanol production would incorporate all essential steps including pretreatment, cellulase production, enzyme hydrolysis and fermentation of the released sugars into ethanol. IBP represents an inexpensive, environmentally friendly, low energy and low capital approach for second-generation ethanol production. This paper reviews the advancements in microbial-assisted pretreatment for the delignification of lignocellulosic substrates, system metabolic engineering for biorefineries and highlights the possibilities of process integration for sustainable and economic ethanol production. 相似文献
130.
Kei-Hoi Cheung Anuj Kumar Michael Snyder Perry Miller 《Functional & integrative genomics》2000,1(1):70-75
Large-scale genome projects require the analysis of large amounts of raw data. This analysis often involves the application
of a chain of biology-based programs. Many of these programs are difficult to operate because they are non-integrated, command-line
driven, and platform-dependent. The problem is compounded when the number of data files involved is large, making navigation
and status-tracking difficult. To demonstrate how this problem can be addressed, we have created a platform-independent Web
front end that integrates a set of programs used in a genomic project analyzing gene function by transposon mutagenesis in Saccharomyces cerevisiae. In particular, these programs help define a large number of transposon insertion events within the yeast genome, identifying
both the precise site of transposon insertion as well as potential open reading frames disrupted by this insertion event.
Our Web interface facilitates this analysis by performing the following tasks. Firstly, it allows each of the analysis programs
to be launched against multiple directories of data files. Secondly, it allows the user to view, download, and upload files
generated by the programs. Thirdly, it indicates which sets of data directories have been processed by each program. Although
designed specifically to aid in this project, our interface exemplifies a general approach by which independent software programs
may be integrated into an efficient protocol for large-scale genomic data processing.
Electronic Publication 相似文献