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141.
Robson Xavier Faria André Luis Almeida Souza Barbara Lima Luis Armando Candido Tietbohl Caio Pinho Fernandes Raquel Rodrigues Amaral Bettina Monika Ruppelt Marcelo Guerra Santos Leandro Rocha 《Journal of bioenergetics and biomembranes》2017,49(6):473-483
Chagas disease is caused by the Trypanosoma cruzi affecting millions of people, and widespread throughout Latin America. This disease exhibits a problematic chemotherapy. Benznidazole, which is the drug currently used as standard treatment, lamentably evokes several adverse reactions. Among other options, natural products have been tested to discover a novel therapeutic drug for this disease. A lot of plants from the Brazilian flora did not contain studies about their biological effects. Restinga de Jurubatiba from Brazil is a sandbank ecosystem poorly studied in relation to plant biological activity. Thus, three plant species from Restinga de Jurubatiba were tested against in vitro antiprotozoal activity. Among six extracts obtained from leaves and stem parts and 2 essential oils derived from leave parts, only 3 extracts inhibited epimastigote proliferation. Substances present in the extracts with activity were isolated (quercetin, myricetin, and ursolic acid), and evaluated in relation to antiprotozoal activity against epimastigote Y and Dm28 Trypanosoma cruzi strains. All isolated substances were effective to reduce protozoal proliferation. Essentially, quercetin and myricetin did not cause mammalian cell toxicity. In summary, myricetin and quercetin molecule can be used as a scaffold to develop new effective drugs against Chagas’s disease. 相似文献
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Caio Coutinho de Souza Jander Matos Guimares Soraya dos Santos Pereira Luis Andr Morais Mariúba 《Experimental biology and medicine (Maywood, N.J.)》2021,246(23):2443
Bacillus subtilis is a successful host for producing recombinant proteins. Its GRAS (generally recognized as safe) status and its remarkable innate ability to absorb and incorporate exogenous DNA into its genome make this organism an ideal platform for the heterologous expression of bioactive substances. The factors that corroborate its value can be attributed to the scientific knowledge obtained from decades of study regarding its biology that has fostered the development of several genetic engineering strategies, such as the use of different plasmids, engineering of constitutive or double promoters, chemical inducers, systems of self-inducing expression with or without a secretion system that uses a signal peptide, and so on. Tools that enrich the technological arsenal of this expression platform improve the efficiency and reduce the costs of production of proteins of biotechnological importance. Therefore, this review aims to highlight the major advances involving recombinant expression systems developed in B. subtilis, thus sustaining the generation of knowledge and its application in future research. It was verified that this bacterium is a model in constant demand and studies of the expression of recombinant proteins on a large scale are increasing in number. As such, it represents a powerful bacterial host for academic research and industrial purposes. 相似文献
143.
Eduardo P. Amaral Simone C. M. Ribeiro Ver?nica R. Lanes Fabrício M. Almeida Marcelle R. M. de Andrade Caio Cesar Barbosa Bomfim érika M. Salles Karina R. Bortoluci Robson Coutinho-Silva Mario H. Hirata José M. Alvarez Elena B. Lasunskaia Maria Regina D'Império-Lima 《PLoS pathogens》2014,10(7)
The purinergic P2X7 receptor (P2X7R) is a sensor of extracellular ATP, a damage-associated molecule that is released from necrotic cells and that induces pro-inflammatory cytokine production and cell death. To investigate whether the innate immune response to damage signals could contribute to the development of pulmonary necrotic lesions in severe forms of tuberculosis, disease progression was examined in C57BL/6 and P2X7R−/− mice that were intratracheally infected with highly virulent mycobacterial strains (Mycobacterium tuberculosis strain 1471 of the Beijing genotype family and Mycobacterium bovis strain MP287/03). The low-dose infection of C57BL/6 mice with bacteria of these strains caused the rapid development of extensive granulomatous pneumonia with necrotic areas, intense bacillus dissemination and anticipated animal death. In contrast, in P2X7R−/− mice, the lung pathology presented with moderate infiltrates of mononuclear leukocytes without visible signs of necrosis; the disease attenuation was accompanied by a delay in mortality. In vitro, the hypervirulent mycobacteria grew rapidly inside macrophages and induced death by a P2X7R-dependent mechanism that facilitated the release of bacilli. Furthermore, these bacteria were resistant to the protective mechanisms elicited in macrophages following extracellular ATP stimulation. Based on this study, we propose that the rapid intracellular growth of hypervirulent mycobacteria results in massive macrophage damage. The ATP released by damaged cells engages P2X7R and accelerates the necrotic death of infected macrophages and the release of bacilli. This vicious cycle exacerbates pneumonia and lung necrosis by promoting widespread cell destruction and bacillus dissemination. These findings suggest the use of drugs that have been designed to inhibit the P2X7R as a new therapeutic approach to treat the aggressive forms of tuberculosis. 相似文献
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Caroline Galgowski Sara Pavanelo Frare Martinho Rau Michele Debiase Alberton Sérgio Althoff Alessandro Guedes Caio Mauricio Mendes de Cordova 《化学与生物多样性》2021,18(1):e2000711
Hydroalcoholic propolis extracts from the bee species Melipona quadrifasciata have been shown to possess antimicrobial activity against different mollicute strains, but a methanolic extract (ME) could contain an increased diversity of nonpolar bioactive components with a potentially higher antimicrobial activity. The ME obtained by maceration of the propolis sample was fractionated with solvents of different polarities and then, purified by silica gel column chromatography through biomonitoring of its antimicrobial activity against mollicute strains. Analysis by gas chromatography-mass spectrometry (GC/MS) enabled the identification of compounds using the NIST library. Minimum inhibitory concentrations (MICs) of the samples were determined by broth microdilution. Anti-adhesive assays were performed with Mycoplasma pneumoniae cells. The hexane (MIC=62.5 mg/L) and dichloromethane (MIC=125 mg/L) fractions presented the most promising results against M. pneumoniae. They were fractionated into 74 subfractions, and even the best ones did not show better results (MIC>250 mg/L) than their original fractions, likely due to the loss of terpene compounds that seem to act in synergy. The dichloromethane subfraction FD4 was highlighted in the anti-adhesive assay with an inhibitory activity of 21.6 %. A synergistic effect of the nonpolar compounds in M. quadrifasciata propolis may be responsible for its antibacterial activity, but several purified components can improve its anti-adhesive properties. 相似文献
146.
Vivian V. Costa Flavio A. Amaral Fernanda M. Coelho Celso M. Queiroz-Junior Bruna G. Malagoli Jose Hugo S. Gomes Fernando Lopes Kátia D. Silveira Daniela Sachs Caio T. Fagundes Lívia D. Tavares Vanessa Pinho Tarcilia A. Silva Mauro M. Teixeira Fern?o C. Braga Danielle G. Souza 《PloS one》2015,10(3)
Rheumatoid Arthritis (RA) is a chronic disease characterized by persistent inflammation and pain. Alternative therapies to reduce these symptoms are needed. Marine algae are valuable sources of diverse bioactive compounds. Lithothamnion muelleri (Hapalidiaceae) is a marine algae with anti-inflammatory, antitumor, and immunomodulatory properties. Here, we investigated the potential anti-inflammatory and analgesic activities of L. muelleri in a murine model of antigen-induced arthritis (AIA) in mice. Our results demonstrate that treatment with L. muelleri prevented inflammation and hypernociception in arthritic mice. Mechanistically, the crude extract and the polysaccharide-rich fractions of L. muelleri may act impairing the production of the chemokines CXCL1 and CXCL2, and consequently inhibit neutrophil influx to the knee joint by dampening the adhesion step of leukocyte recruitment in the knee microvessels. Altogether our results suggest that treatment with L.muelleri has a potential therapeutic application in arthritis treatment. 相似文献
147.
Caio R. Pimentel Ryan Andrades Carlos E. L. Ferreira Otto B. F. Gadig Euan S. Harvey Jean-Christophe Joyeux Tommaso Giarrizzo 《Journal of fish biology》2020,96(2):539-542
Here we present records of sharks obtained using baited remote underwater stereo-video systems (stereo-BRUVS) at two Brazilian oceanic islands. Fourteen of the 60 deployments recorded 19 sharks in Trindade Island. In Saint Peter and Saint Paul Archipelago (SPSPA), two pelagic and two demersal deployments recorded two and one shark, respectively, including the locally extinct Galapagos shark Carcharhinus galapagensis. Stereo-BRUVS should be considered as adjuncts to other non-invasive methods to monitor shark populations. 相似文献
148.
Spatial distance and climate determine modularity in a cross‐biomes plant–hummingbird interaction network in Brazil
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Andréa Cardoso Araujo Ana M. Martín González Brody Sandel Pietro K. Maruyama Erich Fischer Jeferson Vizentin‐Bugoni Francielle Paulina de Araújo Aline Góes Coelho Rogério Rodrigues Faria Glauco Kohler Flor Maria Guedes Las‐Casas Ariadna Valentina Lopes Adriana O. Machado Caio Graco Machado Isabel Cristina Machado Jimmy A. McGuire Alan Cerqueira Moura Genilda M. Oliveira Paulo Eugênio Oliveira Márcia Alexandra Rocca Licléia da Cruz Rodrigues Marcos Rodrigues Ana Maria Rui Ivan Sazima Marlies Sazima Isabela Galarda Varassin Zhiheng Wang Bo Dalsgaard Jens‐Christian Svenning 《Journal of Biogeography》2018,45(8):1846-1858
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Martin Renan Paulo Varela Patricia Gomes Caio Peres Marins Maryana Mara Filippelli-Silva Rafael Yarak Samira Soares Juliana L. M. Sanudo Adriana Idkowiak-Baldys Jolanta Chen Siming Hwang Cheng Zhuang Yong Lyga John Pesquero João Bosco Bagatin Edileia 《Molecular biology reports》2022,49(3):1669-1678
Molecular Biology Reports - Skin aging involves genetic, environmental and hormonal factors. Facial wrinkles also depend on muscular activity. Gene expression investigation may be useful... 相似文献