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741.
Morten Tryland Kjell Handeland Anna-Marie Bratberg Inge-Tom Solbakk Antti Oksanen 《Acta veterinaria Scandinavica》2006,48(1):10-4
To assist in evaluating serological test results from dead animals, 10 silver foxes (Vulpes vulpes) and 10 blue foxes (Alopex lagopus), 6 of each species previously vaccinated against and all challenged with Microsporum canis, were blood sampled and euthanased. Fox carcasses were stored at +10°C, and autopsy was performed on Days 0, 2, 4, 7, and
11 post mortem during which samples from blood and/or body fluid from the thoracic cavity were collected. Antibodies against M. canis were measured in an enzyme-linked immunosorbent assay (ELISA) as absorbance values (optical density; OD). To assess the degradation
of antibodies, the ratio between post mortem and ante mortem absorbance was calculated. The mean absorbance from samples collected during autopsy was generally lower than from samples
from live animals. In blood samples, this difference increased significantly with time (P = 0.04), while in body fluid samples the difference decreased (not significant; P = 0.18). We suggest that a positive serological result from testing blood or body fluid of a dead animal may be regarded
as valuable, although specific prevalences obtained by screening populations based on this type of material may represent
an under-estimation of the true antibody prevalence. Negative serological test results based on material from carcasses may
be less conclusive, taken into account the general degradation processes in decaying carcasses, also involving immunoglobulin
proteins. 相似文献
742.
Antti Kauppila Jukka Puolakka Olavi Ylikorkala 《Prostaglandins & other lipid mediators》1979,18(4):655-661
The prostaglandin biosynthesis inhibitors ketoprofen and indomethacin were compared in the treatment of primary dysmenorrhea in a double-blind, cross-over trial involving 23 patients. Each drug was used for 2–4 days during 3 consecutive menstruations in randomized order. Good or moderate overall relief was obtained in 60 of the 68 ketoprofen-treated menstruations (88 %) and in 60 of the indomethacin-treated cases (90 %). A dysmenorrhea score, based on subjective estimations of 8 symptoms, similarly decreased from a mean (±S.E.M.) basal level of 9.6 ± 0.6 to 3.6 ± 0.3 during ketoprofen treatment and to 4.0 ± 0.3 during indomethacin. Both drugs relieved pelvic and lower back pains and eliminated vomiting and diarrhea in 82–97 % of the cycles whereas headache, fatigue and nervousness were less frequently alleviated (40–67 %). Eighteen of the 23 women (78 %) had been unable to work during the first day of menstruation, the rate of working days lost was reduced to 4 % with ketoprofen and 9 with indomethacin. Mild side-effects occurred during 12 ketoprofen and 14 indomethacin therapies. Ketoprofen thus seems to be as effective and tolerable as indomethacin in the treatment of primary dysmenorrhea. 相似文献
743.
The 13C-n.m.r. spectra have been recorded and assigned for the xylo- and cello-oligosaccharides, the former up to xylopentaose, and the latter up to cello-tetraose. A spectrum of a low-d.p. cellulose in dimethyl sulfoxide-d6 was also assigned. In every instance, the spectra of the higher oligosaccharides closely parallel those of the corresponding disaccharides. Variations in line intensities permitted assignment of peaks to both terminal groups and internal residues. A particularly important difference was observed between the chemical shifts at the internal C-4 atoms for the two series of oligomers. This difference has been interpreted as evidence for significant differences in average linkage-orientation or solvation, which is related to the absence of C-6 in the xylo-oligosaccharides. 相似文献
744.
Deane F. Mosher Olli Saksela Jorma Keski-Oja Antti Vaheri 《Journal of cellular biochemistry》1977,6(4):551-557
Fibronectin was present in media and cell layers of cultures of adherent cells from human skin, kidney, lung, chest wall, liver, and heart. Cell-surface fibronectin, visualized by immunofluorescence, was in dense fibrillar (cultures from lung), discrete fibrillar (e.g., cultures from skin), or punctate (some cultures from kidney) structures. The subunit sizes of cell-surface fibronectin and fibronectin soluble in medium appeared identical in sodium dodecyl sulfate-polyacrylamide gels. To explain the polymorphism of cell-surface fibronectin, there must be chemical differences among the fibronectins synthesized by different cell strains or factors in the cell layer which influence fibronectin binding and aggregation. 相似文献
745.
746.
Genetic analysis of disorder incidence in farmed animals is challenged by two factors. Disorders in different cohorts and environments could be caused by different factors, leading to changes in heritability and to less than unity genetic correlations across cohorts. Moreover, due to computational limitations, liability scale heritabilities at very low incidence may differ from those estimated at higher incidence. We tested whether these two dilemmas occur in skeletal deformations of farmed salmonids using multigeneration data from the Finnish rainbow trout breeding programme and previous salmonid studies. The results showed that heritability was close to zero in cohorts in which management practices maintained incidence at a low level. When there was a management failure and incidence was unusually high, heritability was elevated. This may be due to computational limitations at very low incidence and/or because deformations are induced by different factors in different cohorts. Most genetic correlations between deformations recorded in different generations were weakly to strongly positive. However, also negative correlations between generations were present, showing that high liability at one time can be genetically connected to low liability at another time. The results emphasise that genetic architecture of binary traits can be influenced by trait expression. 相似文献
747.
Abstract. Changes in size and spatial arrangement of plant populations established on an initially bare peat surface were described over a period of 5 yr by following plant individuals on a 1-cm grid in an area of 10 m x 25 m. The spatial pattern of populations and association between species was analyzed statistically. The study site was very slowly colonized by 14 perennial plant species. The early successional stage was dominated by Carex rostrata, with a clumped spatial distribution, and the homogeneously distributed Eriophorum vaginatum and Pinus sylvestris. Both the growth in size of populations and changes in their spatial distribution were interpreted as a result of species dispersal ability, tolerance to severity of the substrate and pattern of reproduction. 相似文献
748.
749.
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