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111.
Enrico Garaci Antonio Mastino Francesca Pica Cartesio Favalli 《Cancer immunology, immunotherapy : CII》1990,32(3):154-160
Summary A combination treatment with thymosin 1 (200 µg/kg) for 4 days, followed by a single injection of murine interferon / (3 × 104 international units/mouse), starting 2 days after cyclophosphamide treatment (200 mg/kg, single injection) demonstrated a dramatic and rapid disappearance of tumor burden in mice bearing Lewis lung carcinoma (3LL) tumor. The effectiveness of this new chemoimmunotherapy protocol was evident even on the long-term survival in a high percentage of animals, and was statistically significant when compared to treatment with the single agents in conjunction with chemotherapy or to chemotherapy itself. The same combination immunotherapy treatment strongly stimulated natural killer activity and cytotoxicity against autologus 3LL tumor cells in 3LL-tumor-bearing mice treated with cyclophosphamide, whereas treatments with each agent singly did not alter or only slightly modified the cytotoxic activity towards Yac-1 or 3LL target cells. Selective depletion with antibodies showed that killer cells stimulated by combination chemoimmunotherapy treatment bear phenotypic characteristics of asialo-GM1-positive cells. A histological study has shown a high number of infiltrating lymphoid cells in the tumors obtained from mice treated with combination chemoimmunotherapy. 相似文献
112.
Antonio Montagnoli R. Kasten Dumroese Mattia Terzaghi Jeremiah R. Pinto Nicoletta Fulgaro Gabriella Stefania Scippa 《Plant biosystems》2018,152(3):515-523
We found that different spectra, provided by light-emitting diodes or a fluorescent lamp, caused different photomorphological responses depending on tree seedling type (coniferous or broad-leaved), species, seedling development stage, and seedling fraction (shoot or root). For two conifers (Picea abies and Pinus sylvestris) soon after germination (≤40 days), more seedling growth was related to a lower ratio of red-to-far-red (R:FR) light. As growth continued to 120 days, spectra with a greater complement of blue light yielded more growth. Roots showed more plasticity to light spectra than shoots. In general for the evergreen broad-leaved Quercus ilex, spectra with additional R:FR than for conifers yielded more growth in the first 57 days. Subsequently as seedlings grew, shoot growth appeared to be influenced less by light source than roots, with root length showing the greatest responses. Our results suggest that manipulating light spectra to foster desired seedling traits may be another tool for use in the production of high-quality seedlings as defined through the Target Plant Concept. Such seedlings are needed for restoration of the two billion hectares of degraded forestland, especially on harsh sites such as those found in the Mediterranean region, and to sequester carbon to mitigate climate change. 相似文献
113.
Isabel Marques Antonio José Díaz‐Pérez José Ángel López‐Rodríguez Victoria Mirones Ana Sus Félix Llamas Alicia Alonso Ernesto Pérez‐Collazos Juan Viruel Elvira Sahuquillo Maria Del Carmen Sancho Benjamin Komac José Antonio Manso José Gabriel Segarra‐Moragues David Draper Luis Villar Pilar Catalán 《Botanical journal of the Linnean Society. Linnean Society of London》2013,173(4):676-706
The Iberian mountain spiny fescues are a reticulate group of five diploid grass taxa consisting of three parental species and two putative hybrids: F. × souliei (F. eskia × F. quadriflora) and F. × picoeuropeana (F. eskia × F. gautieri). Phenotypic and molecular studies were conducted with the aim of determining the taxonomic boundaries and genetic relationships of the five taxa and disentangling the origins of the two hybrids. Statistical analyses of 31 selected phenotypic traits were conducted on individuals from 159 populations and on nine type specimens. Molecular analyses of random amplified polymorphic DNA (RAPD) markers were performed on 29 populations. The phenotypic analyses detected significant differences between the five taxa and demonstrated the overall intermediacy of the F. × picoeuropeana and F. × souliei between their respective parents. The RAPD analysis corroborated the genetic differentiation of F. eskia, F. gautieri and F. quadriflora and the intermediate nature of the two hybrids; however, they also detected genetic variation within F. × picoeuropeana. These results suggest distinct origins for F. × picoeuropeana in the Cantabrian and Pyrenean mountains, with the sporadic Pyrenean populations having potentially resulted from recent hybridizations and the stabilized Cantabrian ones from older events followed by potential displacements of the parents. © 2013 The Linnean Society of London, Botanical Journal of the Linnean Society, 2013, 173 , 676–706. 相似文献
114.
Descriptive analysis of high birth prevalence rate geographical clusters of congenital anomalies in South America 下载免费PDF全文
115.
Elevation as a barrier: genetic structure for an Atlantic rain forest tree (Bathysa australis) in the Serra do Mar mountain range,SE Brazil 下载免费PDF全文
Talita Soares Reis Maísa Ciampi‐Guillardi Miklos Maximiliano Bajay Anete Pereira de Souza Flavio Antonio Maës dos Santos 《Ecology and evolution》2015,5(9):1919-1931
Distance and discrete geographic barriers play a role in isolating populations, as seed and pollen dispersal become limited. Nearby populations without any geographic barrier between them may also suffer from ecological isolation driven by habitat heterogeneity, which may promote divergence by local adaptation and drift. Likewise, elevation gradients may influence the genetic structure and diversity of populations, particularly those marginally distributed. Bathysa australis (Rubiaceae) is a widespread tree along the elevation gradient of the Serra do Mar, SE Brazil. This self‐compatible species is pollinated by bees and wasps and has autochoric seeds, suggesting restricted gene dispersal. We investigated the distribution of genetic diversity in six B. australis populations at two extreme sites along an elevation gradient: a lowland site (80–216 m) and an upland site (1010–1100 m.a.s.l.). Nine microsatellite loci were used to test for genetic structure and to verify differences in genetic diversity between sites. We found a marked genetic structure on a scale as small as 6 km (FST = 0.21), and two distinct clusters were identified, each corresponding to a site. Although B. australis is continuously distributed along the elevation gradient, we have not observed a gene flow between the extreme populations. This might be related to B. australis biological features and creates a potential scenario for adaptation to the different conditions imposed by the elevation gradient. We failed to find an isolation‐by‐distance pattern; although on the fine scale, all populations showed spatial autocorrelation until ~10‐20 m. Elevation difference was a relevant factor though, but we need further sampling effort to check its correlation with genetic distance. The lowland populations had a higher allelic richness and showed higher rare allele counts than the upland ones. The upland site may be more selective, eliminating rare alleles, as we did not find any evidence for bottleneck. 相似文献
116.
Ocadiz-Delgado Rodolfo Cruz-Colin Jose-Luis Alvarez-Rios Elizabeth Torres-Carrillo Antonio Hernandez-Mendoza Karina Conde-Pérezprina Juan-Cristobal Dominguez-Gomez Guadalupe-Isabel Garcia-Villa Enrique Lambert Paul F. Gariglio Patricio 《Journal of physiology and biochemistry》2021,77(4):547-555
Journal of Physiology and Biochemistry - The high-risk human papillomavirus (HR-HPV) E7 oncoprotein appears to be a major determinant for cell immortalization and transformation altering critical... 相似文献
117.
Cell-mediated lymphocytotoxicity was generated in four strain combinations differing only by the cell-surface expression of the class II E molecule controlled by the H-2 complex. The four combinations were: B10.D2(R107) anti-B10.A(3R), B10.A(4R) anti-B10.A(2R), B10.GD anti-B10.D2(R101), and B10.S(7R) anti-B10.S(9R). In all four of these combinations, the stimulator expresses E molecules on the cell surface, while the responder does not. The cytolytic T lymphocytes generated in the B10.D2(R107) anti-B10.A(3R) and B10.A(4R) anti-B10.A(2R) combinations reacted not only with the stimulator but also with strains that do not express cell-surface E molecules, in particular, strains carrying the H-2
f
and H-2
q
haplotypes. The cross-reactivity with E-negative strains could be blocked by monoclonal antibodies specific for the Af or Aq molecules but not by antibodies recognizing determinants on E or class I (K) molecules. The anti-H-2f cross-reactivity could be inhibited by H-2
q
cold targets and, reciprocally, the anti-H-2q reactivity could be blocked by H-2
f
cold targets. These findings are interpreted as indicating that the cytolytic T lymphocytes stimulated by E molecules can recognize and lyse cells lacking E molecules but expressing A molecules. The observed E-A cross-reactivity supports the notion of structural and functional relatedness between the A and E molecules and suggests a common evolutionary origin of the A- and E-encoding loci. 相似文献
118.
Polyamine metabolism and its regulation 总被引:20,自引:1,他引:20
Antonio F. Tiburcio Teresa Altabella Antoni Borrell Carles Masgrau 《Physiologia plantarum》1997,100(3):664-674
119.
The taxonomy of marine species of the genus Diphyllobothrium, particularly those parasitic in cetaceans, is rather confused. During parasitological investigations of long-finned pilot
whales Globicephala melas from waters off the Faroe Islands, five diphyllobothriid species were detected: Diphyllobothrium sp. (possibly D. polyrugosum), D. stemmacephalum, Diphyllobothrium sp. A, Diphyllobothrium sp. B and Diphyllobothriidae sp. D. stemmacephalum is reported for the first time from G. melas. The stituation regarding the taxonomy of Diphylobothrium species from cetaceans is briefly reviewed. It is concluded that the recent development of genetic techniques may be of great
value in relation to clarifying the taxonomy of this group. 相似文献
120.
E Ricciardi M Jakimovska P Maniglio M Schimberni A Frega B Kobal M Moscarini 《World journal of surgical oncology》2012,10(1):177
ABSTRACT: BACKGROUND: Intraoperative injury of the obturator nerve has rarely been reported in patients with gynecological malignancies undergoing extensive radical surgeries. Irreversible damage of this nerve causes thigh paresthesia and claudication. Intraoperative repair may be done by end-to-end anastomosis or grafting when achieving tension-free anastomosis is not possible. CASE PRESENTATION: A 28-year-old woman with stage IB cervical cancer underwent fertility--sparing surgery, including conization and bilateral pelvic lymphadenectomy. The left obturator nerve was damaged intraoperatively during pelvic dissection. CONCLUSION: Immediate laparoscopic repair was successful and there was no functional deficit in the left thigh for six months postoperatively. 相似文献