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991.
MicroRNA-451 Regulates LKB1/AMPK Signaling and Allows Adaptation to Metabolic Stress in Glioma Cells
992.
Corrente M Franchini D Decaro N Greco G D'Abramo M Greco MF Latronico F Crovace A Martella V 《The new microbiologica》2010,33(4):337-341
Brucella spp. is a worldwide zoonotic pathogen. Infection by Brucella canis in dogs is endemic in the Southern USA and in Central and South America, but it appears sporadically in other parts of the world, including Europe. Tissue samples from a dog with chronic prostatitis, discospondylitis and locomotor problems were subjected to clinical and laboratory examinations. B. canis was detected by PCR in biological fluids and tissues of the animal, while antibodies to B. canis were found in the serum, providing additional strong evidence for the circulation of B. canis in Italy. 相似文献
993.
Carmen Aspíroz Yolanda Gilaberte Antonio Rezusta Teun Boekhout Ma Carmen Rubio 《Revista iberoamericana de micología》2010,27(1):20-21
BackgroundMalassezia pachydermatis is a yeast of importance in both veterinary and human medicine.AimsTo know if M. pachydermatis grow on micological media with high concentrations of gentamycin.MethodsTwenty M. pachydermatis strains were streaked on Sabouraud Dextrose Agar plates with different concentrations of gentamycin.ResultsAll isolates were inhibited when high concentrations of gentamycin were added.ConclusionsThe use of plates with high concentrations of gentamycin can lead to some important misdiagnoses: firstly, false-negative cultures, and secondly, an erroneous classification of M. pachydermatis as a lipid-dependent species. Morever, all of this could be useful in two therapeutic fields: i) in animals, topical gentamycin could be an efficacious treatment for a disease such as external otitis in dogs; ii) in humans, we hypothesize that gentamycin could be regarded as a possible therapy (“antibiotic-lock”) for catheter-associated Malassezia spp. infections. 相似文献
994.
Immunostaining for nucleophosmin in bone marrow trephine biopsy specimens in acute myeloid leukemias
995.
Bouke C. de Jong Ifedayo Adetifa Brigitte Walther Philip C. Hill Martin Antonio Martin Ota & Richard A. Adegbola 《FEMS immunology and medical microbiology》2010,58(1):102-105
Mycobacterium africanum (MAF) is a common cause of human pulmonary tuberculosis in West Africa. We previously described phenotypic differences between MAF and Mycobacterium tuberculosis (MTB) among 290 patients. In the present analysis, we compared 692 tuberculosis patients infected with the two most common lineages within the (MTB) complex found in the Gambia, namely MAF West African type 2 (39% prevalence) and Euro-American MTB (55% prevalence). We identified additional phenotypic differences between infections with these two organisms. MAF patients were more likely to be older and HIV infected. In addition, they had worse disease on chest X-ray, despite complaining of cough for an equal duration, and were more likely severely malnourished. In this cohort, the prevalence of MAF did not change significantly over a 7-year period. 相似文献
996.
Laura Tomassoli Antonio Tiberini Massimiliano Meneghini 《Journal of Phytopathology》2010,158(4):314-316
Since 2006, winter melon plants (Cucumis melo L. var inodorus) showing symptoms of pin‐point yellow spots were noticed in Sicily (Italy). Leaf samples were tested by enzyme‐linked immunosorbent assay to the most important viruses‐infecting cucurbits. Zucchini yellow fleck virus (ZYFV, genus Potyvirus) was the only virus detected. Surveys in 2007 and 2008 revealed an increasing number of sites in Sicily with ZYFV‐infected winter melon plants. To confirm the identity of the virus as ZYFV, two isolates from different locations were sequenced and shown to be approximately 85% identical to the published sequences of isolates previously identified in Italy and France. This is the first report of ZYFV occurring on melon in Italy. 相似文献
997.
A. Trabocchi G. Menchi E. Danieli D. Potenza N. Cini A. Bottoncetti S. Raspanti A. Pupi Antonio Guarna 《Amino acids》2010,38(1):329-337
3-Aza-6,8-dioxabicyclo[3.2.1]octane-based amino acids as reverse turn inducers have been introduced into cyclic peptidomimetics
containing the RGD or DGR retro-sequence, in order to achieve a stereochemical scanning of the binding capability of the resulting
molecules towards αvβ3 and αvβ5 integrins, resulting in retro-inverso DGR peptides as micromolar ligands. A comparative analysis between the conformational
preferences of 4 and of its isomer 3, having the opposite RGD sequence, was reported with respect to the binding activity, giving insight into the factors affecting
the preferential binding of 4 to the αvβ5 integrin. 相似文献
998.
Benedetta Gualeni Marcella Facchini Fabio De Leonardis Ruggero Tenni Giuseppe Cetta Manuela Viola Alberto Passi Andrea Superti-Furga Antonella Forlino Antonio Rossi 《Matrix biology》2010,29(6):453-460
Mutations in the sulfate transporter gene, SCL26A2, lead to cartilage proteoglycan undersulfation resulting in chondrodysplasia in humans; the phenotype is mirrored in the diastrophic dysplasia (dtd) mouse. It remains unclear whether bone shortening and deformities are caused solely by changes in the cartilage matrix, or whether chondroitin sulfate proteoglycan undersulfation affects also signalling pathways involved in cell proliferation and differentiation. Therefore we studied macromolecular sulfation in the different zones of the dtd mouse growth plate and these data were related to growth plate histomorphometry and proliferation analysis.A 2-fold increase of non-sulfated disaccharide in dtd animals compared to wild-type littermates in the resting, proliferative and hypertrophic zones was detected indicating proteoglycan undersulfation; among the three zones the highest level of undersulfation was in the resting zone. The relative height of the hypertrophic zone and the average number of cells per column in the proliferative and hypertrophic zones were significantly reduced compared to wild-types; however the total height of the growth plate was within normal values. The chondrocyte proliferation rate, measured by bromodeoxyuridine labelling, was also significantly reduced in mutant mice. Immunohistochemistry combined with expression data of the dtd growth plate demonstrated that the sulfation defect alters the distribution pattern, but not expression, of Indian hedgehog, a long range morphogen required for chondrocyte proliferation and differentiation.These data suggest that in dtd mice proteoglycan undersulfation causes reduced chondrocyte proliferation in the proliferative zone via the Indian hedgehog pathway, therefore contributing to reduced long bone growth. 相似文献
999.
Shailesh Sharma Gabriele Cavallaro Antonio Rosato 《Journal of biological inorganic chemistry》2010,15(4):559-571
Multiheme c-type cytochromes (MHCs) are metalloproteins that can play various biochemical roles, including enzymatic activity and electron
transfer. As electron transfer proteins, the presence of multiple heme cofactors in the vicinity allows electrons to rapidly
travel relatively long distances. MHCs are often characterized by relatively low structural complexity, with the heme cofactors
being largely responsible for maintaining the structure in place, owing to the protein–heme covalent linkages. In this work,
we analyzed an extensive ensemble of 594 complete prokaryotic proteomes, amounting to more than 1.9 million sequences, to
characterize their content in MHCs. We identified 1,659 MHCs in 258 organisms. The presence of MHCs was found to correlate
quite well with the capability of an organism to synthesize or take up heme. For two organisms, the presence of MHCs in the
proteome could be taken as a hint to the presence of divergent heme uptake pathways. The most common numbers of heme-binding
motifs in a sequence were four (25%) and two (23%), followed by five (13%) and ten (9.8%). The average protein-to-heme ratio
was relatively similar for all MHCs, except diheme proteins, regardless of the number of motifs at around 60 ± 30. The latter
ratio could in favorable cases be a useful indicator for functional assignments of novel MHCs. Finally, we showed that the
amount of structural information currently available for MHCs is limited with respect to the diversity of this broad class
of metalloproteins. Experimental efforts in the structural investigation of MHCs are thus warranted. 相似文献
1000.
Daniel Gómez Patricia Lucas‐Elío Francisco Solano Antonio Sanchez‐Amat 《Molecular microbiology》2010,75(2):462-473
The melanogenic marine bacterium Marinomonas mediterranea synthesizes a novel antimicrobial protein (LodA) with lysine‐epsilon oxidase activity (EC 1.4.3.20). Homologues to LodA have been detected in several Gram‐negative bacteria, where they are involved in biofilm development. Adjacent to lodA is located a second gene, lodB, of unknown function. This genomic organization is maintained in all the microorganisms containing homologues to these genes. In this work we show that lodA and lodB constitute an operon. Western blot analysis and enzymatic determinations revealed that LodA is secreted to the external medium when the culture reaches the stationary phase. LodB, on the other hand, has only been detected inside cells, but it is not secreted. The expression of the lysine‐epsilon oxidase (LOD) activity in M. mediterranea requires functional copies of both genes since mutants lacking either lodA or lodB do not show any LOD activity. The active form of LodA containing the quinonic cofactor is intracellularly generated in a process that takes place only in the presence of LodB, suggesting that the latter is involved in this process. Moreover, in the absence of one of the proteins, the stability of the partner protein is compromised leading to a marked decrease in its cellular levels. 相似文献