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41.
42.
Chris M. van der Loos Anton E. Becker Joost J. van den Oord 《The Histochemical journal》1993,25(1):1-13
Summary Many methodologies exist to perform an immunoenzyme double staining. Hence, the practical problem arises as to which of these methods is optimal for one's own experimental design. A process of selection is described which is derived from our own practical experience. First, a general strategy is outlined for the handling of tissue sections to be used for multiple staining methods. Secondly, the selection of an appropriate immunoenzyme double-staining concept is made using a flow chart. Thereafter we give criteria for the definitive selection of an immunoenzyme double-staining protocol based on the characteristics of the tissue or cell type under study. Particular attention is given to the selection of appropriate detection systems, applying enzymes or gold particles, and good contrasting colour combinations. The problems of visualizing co-localization using immunoenzyme double staining are dealt with, and suggestions are made to adapt the method, if necessary, in order to optimize it.This paper (in modified form) is part of the thesis of C. M. van der Loos: Free University Press 1992, Amsterdam, The Netherlands (ISBN 90-5383-081-2). 相似文献
43.
Martine de Boer Maaike te Lintel Hekkert Jiang Chang Bibi S. van Thiel Leonie Martens Maxime M. Bos Marion G. J. de Kleijnen Yanto Ridwan Yanti Octavia Elza D. van Deel Lau A. Blonden Renata M. C. Brandt Sander Barnhoorn Paula K. Bautista-Niño Ilona Krabbendam-Peters Rianne Wolswinkel Banafsheh Arshi Mohsen Ghanbari Christian Kupatt Leon J. de Windt A. H. Jan Danser Ingrid van der Pluijm Carol Ann Remme Monika Stoll Joris Pothof Anton J. M. Roks Maryam Kavousi Jeroen Essers Jolanda van der Velden Jan H. J. Hoeijmakers Dirk J. Duncker 《Aging cell》2023,22(3):e13768
44.
Shau-Ting Chiu Lori H. Anton Frank W. Ewers Raymond Hammerschmidt Kurt S. Pregitzer 《American journal of botany》1992,79(2):149-154
Fertilized stands of Pseudotsuga menziesii were found to have glaucous needles. We investigated the morphological and quantitative characteristics of the epicuticular waxes of needles of fertilized and control trees. Glaucousness was caused by ornate tubular epicuticular wax. Dipping needles in chloroform, which dissolves waxes, eliminated the glaucous appearance. Based on cryostage scanning electron microscopic observations, the epicuticular waxes in the nonstomatal region were much more ornate on the needles of the fertilized trees (experimental needles) than in unfertilized trees (control needles). The stomatal region in both experimental and control needles showed similarly ornate waxes. Quantities of waxes were similar in experimental and control needles. The glaucousness was not the result of greater quantities of wax; rather, fertilization altered wax morphology in the nonstomatal regions. 相似文献
45.
Anton Hofreiter 《Nordic Journal of Botany》2004,24(2):117-141
The subgenus Sphaerine of Bomarea (Alstroemeriaceae) recently contains 33 validly published names. Extended field studies in Peru and extensive investigation of herbarium material revealed the urgent necessity for a revision. As a result, the number of species is reduced to 12, among them two species are newly described. 9 species are members of an other subgenus. A key to determine the species is given in English and Spanish. The typical growth form and its variability, habitat preferences and distribution are discussed separately for each species. 相似文献
46.
47.
Anton Stefan Reiter und Gerhard Loupal 《Journal of Ornithology》1995,136(2):221-223
In July 1992, in the Austrian part of Hanság, a seventy day old young bustard was found dead in a grassland. On the left intertarsal joint a walnut sized open pock was located. Other pocks reaching pea-size were found on both legs. The diagnosis pox was established by light- and electron-microscopic examination of the lesions. A further chick of another hen, fledged in the same year, observed from a distance showed abnormal thickening of the intertarsal joint area. The consequences of pox for such a small group of Great Bustards (total for 1988–1993 15–20 birds) should be watched carefully. 相似文献
48.
Isolation of Com1, a New Gene Required to Complete Meiotic Double-Strand Break-Induced Recombination in Saccharomyces Cerevisiae 总被引:1,自引:1,他引:0 下载免费PDF全文
We have designed a screen to isolate mutants defective during a specific part of meiotic prophase I of the yeast Saccharomyces cerevisiae. Genes required for the repair of meiotic double-strand breaks or for the separation of recombined chromosomes are targets of this mutant hunt. The specificity is achieved by selecting for mutants that produce viable spores when recombination and reductional segregation are prevented by mutations in SPO11 and SPO13 genes, but fail to yield viable spores during a normal Rec(+) meiosis. We have identified and characterized a mutation com1-1, which blocks processing of meiotic double-strand breaks and which interferes with synaptonemal complex formation, homologous pairing and, as a consequence, spore viability after induction of meiotic recombination. The COM1/SAE2 gene was cloned by complementation, and the deletion mutant has a phenotype similar to com1-1. com1/sae2 mutants closely resemble the phenotype of rad50S, as assayed by phase-contrast microscopy for spore formation, physical and genetic analysis of recombination, fluorescence in situ hybridization to quantify homologous pairing and immunofluorescence and electron microscopy to determine the capability to synapse axial elements. 相似文献
49.
Andrew M. Jackson Anton B. Alexandrov S. Prescott Keith James 《Cancer immunology, immunotherapy : CII》1995,40(2):119-124
Intravesical immunotherapy for bladder cancer is the most effective form of tumour immunotherapy. Following repeated instillations
of bacillus Calmette-Guérin (BCG) organisms into the bladder large 0quantities of several cytokines are detected in the urine.
These cytokines include interleukins IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, tumour necrosis factor α (TNFα), interferon γ (IFNγ)
and also soluble intercellular adhesion molecule ICAM-1. In the work reported here we simultaneously quantified urinary levels
of TNFα, TNFβ, TNF receptor I and TNF receptor II by enzyme-linked immunosorbent assay (ELISA) techniques and compared this
with bioactive levels of TNF. This was undertaken with a limited number of patients throughout a course of six instillations
of immuno therapy. Sequential instillations of BCG induced secretion of TNFα and TNFβ into urine. These cytokines were not
always secreted simultaneously, perhaps suggesting differential regulation of their synthesis. Maximal concentrations of TNFα
were 675 pg/ml and TNFβ 47 pg/ml. High levels of both species of soluble TNF receptor were readily identified in urine. Maximal
levels of sTNF-RI were 6200 pg/ml (range from 0) and for sTNF-RII 7800 pg/ml (range from 0). Contrasting with earlier published
observations concerning cytokine levels, the concentration of soluble receptor did not increase with repeated instillation.
In apparent contrast with the ELISA data, very low levels of bioactive TNF were identified by the L929 bioassay (maximum concentration
1 U/ml) despite the elevated concen t ration of immunoreactive TNF. The large concentrations of soluble TNF receptor in patients’
urine samples could account for the apparently low bioactivity as determined by the L929 cytotoxicity assay. The precise nature
of the role of TNF in BCG immunotherapy remains undetermined; however, it is thought that proinflammatory cytokines are in
part responsible for the clinical efficacy of this therapeutic approach. Whether other cytokines are antogonised by soluble
binding proteins remains to be determined. Furthermore, whether TNF is bioactive in the bladder wall and only neutralised
in the urine also requires investigation.
Received: 24 August 1994 / Accepted: 17 October 1994 相似文献
50.
Christoph Winterhalter Peter Heinrich Anton Candussio Günther Wich Wolfgang Liebl 《Molecular microbiology》1995,15(3):431-444
A segment of Thermotoga maritima strain MSB8 chromosomal DNA was isolated which encodes an endo-1,4-β-D-xylanase, and the nucleotide sequence of the xylanase gene, designated xynA, was determined. With a half-life of about 40 min at 90°C at the optimal pH of 6.2, purified recombinant XynA is one of the most thermostable xylanases known. XynA is a 1059-amino-acid (?120 kDa) modular enzyme composed of an N-terminal signal peptide and five domains, in the order A1-A2-B-C1-C2. By comparison with other xylanases of family 10 of glycosyl hydrolases, the central ?340-amino-acid part (domain B) of XynA represents the catalytic domain. The N terminal ?150-amino-acid repeated domains (A1-A2) have no significant similarity to the C-terminal ?170-amino-acid repeated domains (C1-C2). Cellulose-binding studies with truncated XynA derivatives and hybrid proteins indicated that the C-terminal repeated domains mediate the binding of XynA to microcrystalline cellulose and that C2 alone can also promote cellulose binding. C1 and C2 did not share amino acid sequence similarity with any other known cellulose-binding domain (CBD) and thus are CBDS of a novel type. Structurally related protein segments which are probably also CBDs were found in other multi-domain xylanolytic enzymes. Deletion of the N-terminal repeated domains or of all the non-catalytic domains resulted In substantially reduced tbermostability while a truncated xylanase derivative lacking the C-terminal tandem repeat was as thermostable as the full-length enzyme. It is argued that the multidomain organization of some enzymes may be one of the strategies adopted by thermophiles to protect their proteins against thermal denaturation. 相似文献